Homopyrimidine oligonucleotides modified by a pyrenylmethyl group at the terminal position: Enhanced fluorescence upon binding to double helical DNA

Chemistry Letters

Volumn , Issue 11, 1997, Pages 1173-1174

  Yamana, Kazushige ^a   Kumamoto, Satoshi ^a   Nakano, Hidehiko ^a  

^a UNIVERSITY OF HYOGO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0031314317     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.1997.1173     Document Type: Article

References (24)

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17. MALDI-TOFF Mass data [found(calcd)]: oligomer 1, 3726.2 (3726.5); oligomer 2, 3422.3(3422.3); oligomer 3, 3454.3(3454.3).
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24. It is not precluded that the stacking interactions between the attached pyrene and the nucleic acid bases of DNA, in part, contribute to the stability and fluorescence of the triple helix. The fluorescence enhancement appears to be correlated to the relative stability of the triplex helix. The most stabilized triplex (oligomer 3, ΔTm = 7.9°C) exhibited less fluorescence enhancement (Ft/Fs = 2.0) when compared with oligomer 2 (ΔTm = 2.6°C, Ft/Fs = 4.0).