The "Comet" Assay for Detection of Potential Genotoxicity of Polluted Water

Folia Biologica

Volumn 45, Issue 3-4, 1997, Pages 153-156

  Kosz Vnenchak, Magdalena ^a   Rokosz, Katarzyna ^a  

^a JAGIELLONIAN UNIVERSITY   (Poland)

Author keywords

Comet assay; Hep G2 cells; Industrial plants; Water pollutants

Indexed keywords

AGAR GEL ELECTROPHORESIS; ARTICLE; CELL CULTURE; DNA DAMAGE; GENETICS; HUMAN; IN VITRO STUDY; INDUSTRIAL WASTE; LIVER CELL CARCINOMA; LIVER TUMOR; METHODOLOGY; MUTAGEN TESTING; WATER POLLUTANT;

CARCINOMA, HEPATOCELLULAR; DNA DAMAGE; ELECTROPHORESIS, AGAR GEL; HUMANS; INDUSTRIAL WASTE; LIVER NEOPLASMS; MUTAGENICITY TESTS; TUMOR CELLS, CULTURED; WATER POLLUTANTS, CHEMICAL;

EID: 0031306357     PISSN: 00155497     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. CARRANO A. W., NATARAJAN A. T. 1988. Consideration for population monitorinng using cytogenetic techniques. Mutat. Res. 204: 379-406.
2. DARROUDI F., NATARAJAN A. T. 1993. Metabolic activation of chemicals to mutagenic carcinogens by human hepatoma microsomal extracts in Chinese hamster ovary cells (in vitro). Mutagenesis 8: 11-15.
3. ECKL P. 1988. Short-term genotoxicity screening of water samples with primary cultures of rat hepatocytes - preliminary results. Ber. Nat. Med. Ver. (Salzburg) 9: 21-28.
4. ELLIS D., JONE C., LARSON R., SCHAEFFER D. 1982. Organic constituents of mutagenic secondary effluents from waste treatment plants. Arch. Environ. Contam. Toxicol. 11: 373-382.
5. HELMA C., SOMMER R., SCHULTE-HERMANN R., KNASMÜLLER S. 1994. Enhance clastogenicity of contaminated groundwater following UV irradiation detected by the Tradescantia micronucleus assay. Mutat. Res. 323: 93-98.
6. LAUDE M., ERIKSSON S., NYGREN J., AHNSTROM G. 1996. The comet assay: mechanisms and technical consideration. Mutat. Res. 363: 89-96.
7. KOPPEN G., VERSCHAEVE L., 1996. The alkaline comet test on plant cells: a new genotoxicity test for DNA strand breaks in Vicia faba root cells. Mutat. Res. 360: 193-200.
8. KOSZ-VNENCHAK M., ROKOSZ K., SZUSTER A., KUBIAK R. 1996. Use of micronucleus test for screening the potential genotoxicity of polluted water. Centr. Europ. J. Occup. Environm. Med. 2: 329-334.
9. KUBIAK R. 1995. Environmental biomonitoring - general overview. (In: Molecular Biology for Man and Environment. M. Gumińska, J. Mie(combining ogonek)dzobrodzki eds. Uniwersytet Jagielloński, Kraków): 11-20.
10. KÖTELES G. J. 1996. The human lymphocyte micronucleus assay. A review on its applicabilities in occupational and environmental medicine. Centr. Europ. J. Occup. Environm. Med. 2: 12-30.
11. NATARAJAN A. T., TATES A. D., VAN BULL P. P. W., MEIJERS M., DE VOGEL N. 1976. Cytogenetic effect of mutagen/carcinogen after activation in a microsomal system in vitro. I. Induction of chromosome aberration and sister-chromatid exchanges by diethylnitrosamine(DEN) and dimethylnitrosamine(DMN) in CHO cells in the presence of rat liver microsomes. Mutat. Res. 37: 83-90.
12. NATARAJAN A. T., DARROUDI F. 1991. Use of human hepatoma cells for in vitro metabolic activation of chemical mutagens/carcinogens. Mutagenesis 6: 399-403.
13. OLIVE P. L., BANÁTH J. P. 1993. Induction and rejoining of radiation-induced DNA single-strand breaks: "tail moment" as a function of position in the cell cycle. Mutat. Res. DNA Repair 294: 275-283.
14. OLIVE P. L., BANÁTH J. P. 1993. Detection of DNA double-strand breaks through the cell cycle after exposure to X-rays, bleomycin, etopside and 125 IdUrd. Int. J. Radiat. Biol. 64: 349-358.
15. OSTLING O., JOHANSON K. J. 1984.Microelectrophoresis study of radiation-induced DNA damages in individual cells. Biochem. Biophys. Res. Commun. 123: 291-298.
16. RALPH S., PETRAS M., PANDRANGI R., VRZOC M. 1996. Alkaline single-cell gel (comet) assay and genotoxicity monitoring using two species of tadpoles. Environ. Mol. Mutagen. 28: 112-120.
17. SALAGOVIC J., GILLES J., VERSCHAEVE L., KALINA I. 1996. The comet assay for the detection of genotoxic damage in the earthworms: a promising tool for assessing the biological hazards of polluted sites. Folia biol. (Praha) 42: 17-21.
18. SAYATO Y., NAKAMURO K., UENO H., GOTO R. 1990. Mutagenicity of adsorbates to a copper-phthalocyanine derivative recovered from municipal river water. Mutat. Res. 242: 313-317.
19. SORSA M., HAYASHI M., NORPPA H. VAINIO H. 1990. Human biomonitoring in exposure to environmental genotoxicants. (In: Antimutagenesis and Anticancerogenesis Mechanism. Y. Kuroda, D. M. Shankel, M. D. Waters eds. II Plenum Press, New York, London): 79-86.
20. TAFAZOLI M., KIRSCH-VOLDERS M. 1996. In vitro mutagenicity and genotoxicity study of 1,2-dichloroethylene, 1,1,2-trichloroethylene, 1,3-dichloropropane, 1,2,3-trichloropropane and 1,1,3-trichloropropene, using the micronucleus test and the alkaline single cell gel electrophoresis technique (comet assay) in human lymphocytes. Mutat. Res. 371: 185-202.
21. TURCHI G., NARDONE A., PALITTI F. 1992. Application of an epithelial liver cell line, metabolically competent, for mutation studies of promutagens. Mutat. Res. 271: 127-135.