In vitro mutagenicity and genotoxicity study of 1,2-dichloroethylene, 1,1,2-trichloroethane, 1,3-dichloropropane, 1,2,3-trichloropropane and 1,1,3-trichloropropene, using the micronucleus test and the alkaline single cell gel electrophoresis technique (comet assay) in human lymphocytes

Mutation Research - Genetic Toxicology

Volumn 371, Issue 3-4, 1996, Pages 185-202

  Tafazoli, M ^a   Kirsch Volders, M ^a  

^a FREE UNIVERSITY OF BRUSSELS   (Belgium)

Author keywords

Chlorinated aliphatic hydrocarbon; Comet assay; Human lymphocyte; In vitro; Micronucleus test; Mutagenicity and genotoxicity assay

Indexed keywords

1,1,2 TRICHLOROETHANE; 1,1,3 TRICHLOROPROPYLENE; 1,2 DICHLOROETHYLENE; 1,2,3 TRICHLOROPROPANE; 1,3 DICHLOROPROPANE; CHLORINATED HYDROCARBON; CYCLOPHOSPHAMIDE; MITOMYCIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CYTOTOXICITY; GEL ELECTROPHORESIS; GENOTOXICITY; HUMAN; HUMAN CELL; LYMPHOCYTE; MICRONUCLEUS TEST; MUTAGENICITY; PRIORITY JOURNAL;

EID: 0030596086     PISSN: 01651218     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-1218(96)90107-X     Document Type: Article

References (46)

1. Birner G., Vamvakas S., Dekant W., Henschler D. Nephrotoxic and genotoxic N-acetyl-S-dichlorovinyl-L-cycteine is a urinary metabolite after occupational 1,1,2-trichloroethene exposure in humans: implications for the risk of trichloroethene exposure. Environ. Health Perspect. 99:1993;281-284.
2. Allen J.W., Collins B.W., Evansky P.A. Spermatid micronucleus analyses of trichloroethylene and chloral hydrate effects in mice. Mutation Res. 323:1994;81-88.
3. Forment O., Boivin S., Barbin A., Bancel B., Trepo C., Marion M-J. Mutagenesis of ras proto-oncogenes in rat liver tumors induced by vinyl chloride. Cancer Res. 54:1994;5340-5345.
4. Weisburger E.K. Carcinogenicity studies on halogenated hydrocarbons. Environ. Health Perspect. 21:1997;7-16.
5. National Toxicology Program. Toxicology and carcinogenesis studies of 1,2,3-trichloropropane (CAS No. 96-18-4) in F344/N Rats and B6C3F1 Mice (Gavage studies). NTP TR 384. 1993;Research Triangle Park, NC.
6. Storer R.D., Cartwright M.E., Cook W.O., Soper K.A., Nichols W.W. Short-term carcinogenesis bioassay of genotoxic procarcinogens in PIM Transgenic mice. Carcinogenesis. 16:1995;285-293.
7. Irwin R.D., Haseman J.K., Eustis S.L. 1,2,3-Trichloropropane: A multisite carcinogen in rats and mice. Fundam. Appl. Toxicol. 25:1995;241-252.
8. Nagano M., Yamamoto H., Harada K., Miyamoto E., Futatsuka M. Comparative study of modification and degradation of neurofilament proteins in rats subchronically treated with allyl chloride, acrylamide, or 2,5-hexanedione. Environ. Res. 63:1993;229-240.
9. 1 mice. Environ. Health. Perspect. 101:(Suppl. 5):1993;271-276.
10. Brittebo E.B., Darnerud P.O., Eriksson C., Brandt I. Nephrotoxicity and covalent binding of 1,1-dichloroethylene in buthionine sulphoximine-treated mice. Arch. Toxicol. 67:1993;605-612.
11. Dees C., Travis C. Trichloroacetate stimulation of liver DNA synthesis in male and female mice. Toxicol. Lett. 70:1994;343-355.
12. Guengerich F.P., Crawford W.M., Jeanne J.R., Domoradzki J.Y., Macdonald T.L., Watanabe. In vitro activation of 1,2-dichloroethane by microsomal and cytosolic enzymes. Toxicol. Appl. Pharmacol. 55:1980;303-317.
13. Invanetich K.M., Van Den Honert L.H. Chloroethanes: their metabolism by hepatic cytochrome P-450 in vitro. Carcinogenesis. 2:1981;697-702.
14. Blocki F.A., Logan M.S.P., Baoli C., Wackeh L.P. Reaction of rat liver glutathione S-transferase and bacterial dichloromethane dehalogenase with dihalomethanes. J. Biol. Chem. 269:1994;8826-8830.
15. Fench M., Morley A.A. Measurement of micronuclei in lymphocytes. Mutation Res. 147:1985;29-36.
16. Van Hummelen P., Kirsch-Volders M. An improved method for the in vitro micronucleus test on isolated human lymphocytes. Mutation Res. 291:1994;93-102.
17. Elhajouji A., Santos A.P., Van Hummelen P., Kirsch-Volders M. Metabolic differences between whole blood and isolated lymphocyte cultures for micronucleus (MN) induction by cyclophosphamide and benzo[ a ]pyrene. Mutagenesis. 9:1994;307-313.
18. Erexson G.L., Bryant M.F., Kwanyuen P., Kligerman A.D. Bleomycin sulfate-induced micronuclei in human, rat and mouse peripheral blood lymphocytes. Environ. Mol. Mutagenesis. 23:1995;31-36.
19. Surrallés J., Xamena N., Creus A., Marcos R. The suitability of the micronucleus assay in human lymphocytes as a new biomakers of excision repair. Mutation Res. 342:1995;43-59.
20. Singh N.P., McCoy M.T., Tice R.R., Schneider E.L. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp. Cell Res. 175:1988;184-191.
21. Tice R.R., Andrews P.W., Hirai O., Singh N.P. The single cell gel (SCG) assay: an electrophoretic technique for the detection of DNA damage in individual cells. Witmer C.R., Snyder R.R., Jollow D.J., Kalf G.F., Kocsis J.J., Sipes I.G. Biological reactive intermediates IV, Molecular and cellular effects and their impact on human health. 1991;157-164 Plenum Press, New York.
22. McKelvey-Martin V.J., Green M.H.L., Schmezer P., Pool-Zobel B.L., De-Meo M.P., Collins A. The single cell gel electrophoresis assay (comet assay): A European review. Mutation Res. 288:1993;47-63.
23. Olive P.L., Frazer G., Banàth J.P. Rapid communication. Radiation-induced apoptosis measured in TK6 human B lymphoblast cell using the comet assay. Radiat Res. 136:1993;130-136.
24. Betti C., Davini T., Giannessi L., Loprieno N., Barale R. Microgel electrophoresis assay (comet test) and SCE analysis in human lymphocytes from 100 normal subjects. Mutation Res. 307:1994;323-333.
25. Hatmann A., Speit G. Genotoxic effects of chemicals in the single cell gel (SCG) test with human blood cells in relation to the induction of sister-chromatid exchanges (SCE). Mutation Res. 346:1995;49-56.
26. Hartmann A., Herkommer K., Glück M., Speit G. DNA-damaging effect of cyclophosphamide on human blood cells in vivo and in vitro studied with the single-cell gel test (comet assay). Environ. Mol. Mutagen. 25:1995;180-187.
27. Tice R.R., Strauss G.H.S., Peters W.P. High-dose combination alkylating agents with autologous bone-marrow support in patients with breast cancer: preliminary assessment of DNA damage in individual peripheral blood lymphocytes using the single cell gel electrophoresis assay. Mutation Res. 271:1992;101-113.
28. Weber G.L., Sipes I.G. In vitro metabolism and bioactivation of 1,2,3-trichloropropane. Toxicol. Appl. Pharmacol. 113:1992;152-158.
29. Raucy J.L., Kraner J.C., Laster J.M. Bioactivation of halogenated hydrocarbons by cytochrome P4502E1. Crit. Rev. Toxicol. 23:1993;1-20.
30. Bronzetti G., Bauer C., Corsi C., Del Carratore R., Galli A., Nieri R., Paolini M., Cundari E., Forti G.C., Crenshaw J. Comparative genetic activity of cis- and trans-1,2-dichloroethylene in yeast. Teratogen. Carcinogen. Mutagen. 4:1984;365-375.
31. Greim H., Bonse G., Radwan Z., Reichert D., Henschler D. Mutagenicity in vitro and potential carcinogenicity of chlorinated ethylenes as a function of metabolic oxiran formation. Biochem. Pharmacol. 24:1975;2013-2017.
32. Koch R., Schlegelmilch R., Wolf H.U. Genetic effects of chlorinated ethylenes in the yeast Sacchromyces cerevisiae. Mutation Res. 206:1988;209-216.
33. Di Renzo A.B., Gandolfi A.J., Sipes I.G. Microsomal bioactivation and covalent binding of aliphatic halides to DNA. Toxicol. Lett. 11:1982;243-245.
34. Crebelli R., Anddreoli C., Carere A., Conti G., Conti L., Cotta Ramusino M., Benigni R. The induction of mitotic chromosome malsegregation in Aspergillus nidulans. Quantitative structure activity relationship (QSAR) analysis with chlorinated aliphatic hydrocarbons. Mutation Res. 266:1992;117-134.
35. Stolzenberg S.J., Hine C.H. Mutagenicity of 2- and 3-carbon halogenated compounds in the Salmonella/mammalian-microsome test. Environ. Mutagen. 2:1980;59-66.
36. Haworth S., Lawlor T., Mortelmans K., Speck W., Zeiger E. Salmonella mutagenicity test results for 250 chemicals. Environ. Mutagen. 5:(Suppl. 1):1983;3-142.
37. Von der Hude W., Scheutwinkel M., Gramlich U., Fissler B., Basler A. Genotoxicity of Three-carbon compounds evaluated in the SCE test. in vitro. Environ. Mutagen. 9:1987;401-410.
38. Ratpan F., Plaumann H. Mutagenicity of halogenated propanes and their methylated derivatives. Environ. Mol. Mutagen. 12:1988;253-259.
39. Doherty A.T., Ellard S., Parry E.M., Parry J.M. An investigation into the activation and deactivation of chlorinated hydrocarbons to genotoxins in metabolically competent human cells. Mutagenesis. 11:1996;247-274.
40. Rosenkranz H.S., Zhang Y.P., Klopman G. Evidence that cell toxicity may contribute to the genotoxic response. Regul. Toxicol. Pharmacol. 19:1994;176-182.
41. Fairbairn D.W., Olive P.L., O'Neill K.L. The comet assay: A comprehensive review. Mutation Res. 339:1995;37-59.
42. Olive P.L., Banàth J.P., Durand R.E. Heterogeneity in radiation-induced DNA damage and repair in tumour and normal cells measured using the comet assay. Radiat. Res. 122:1990;86-94.
43. Ashby J., Tinwell H., Lefevre P.A., Browne M.A. The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and tain moment. Mutagenesis. 10:1995;85-90.
44. Henschler D. Metabolism and mutagenicity of halogenated olefins: A comparison of structure and activity. Environ. Health. Perspect. 21:1977;61-64.
45. Tennant R.W., Spalding J., Stasiewicz S., Ashby J. Prediction of the outcome of rodent carconogenicity bioassays currently being conducted on 44 chemicals by the National Toxicology Programe. Mutagenesis. 5:1990;3-14.
46. Ashby J., Tennant R.W. Difinitive relationships among chemical structure, carcinogenicity and mutagenicity for 301 chemicals tested by the U.S. National Toxicology Program. Mutation Res. 257:1991;229-306.