V(L):V(H) domain rotations in engineered antibodies: Crystal structures of the fab fragments from two murine antitumor antibodies and their engineered human constructs

Proteins: Structure, Function and Genetics

Volumn 29, Issue 2, 1997, Pages 161-171

  Banfield, M J ^a   King, D J ^b   Mountain, A ^b,c   Brady, R L ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b UCB PHARMA   (Belgium)

^c KEELE UNIVERSITY   (United Kingdom)

Author keywords

Antibody humanization; Complementarity determining region; Protein; Quaternary structure

Indexed keywords

CANCER ANTIBODY; IMMUNOGLOBULIN F(AB) FRAGMENT; MONOCLONAL ANTIBODY; ANTIBODY;

ANIMAL CELL; ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ANTIGEN SPECIFICITY; ARTICLE; CRYSTAL STRUCTURE; GENETIC ENGINEERING; HUMAN; HUMAN CELL; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN QUATERNARY STRUCTURE; PROTEIN STRUCTURE; ANIMAL; CHEMISTRY; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; PROTEIN CONFORMATION; PROTEIN ENGINEERING; X RAY CRYSTALLOGRAPHY;

ANIMALS; ANTIBODIES; CRYSTALLOGRAPHY, X-RAY; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MICE; PROTEIN CONFORMATION; PROTEIN ENGINEERING;

EID: 0031253786     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0134(199710)29:2<161::AID-PROT4>3.0.CO;2-G     Document Type: Article

References (29)

1. Winter, G., Milstein, C. Man-made antibodies. Nature 349:293-299, 1991.
2. Colman, P.M. Structure of antibody - antigen complexes: implications for immune recognition. Adv. Immunol. 43:99-132, 1988.
3. Bhat, T.N., Bentley, G.A., Boulot, G., Greene, M.I., Tello, D., Dall'acqua, W., Souchon, H., Schwarz, F.P., Mariuzza, R.A., Poljak, R.J. Bound water molecules and conformational stabilisation help mediate an antigen-antibody association. Proc. Natl. Acad. Sci., U.S.A. 91:1089-1093, 1994.
4. Rini, J.M., Schulze-Garmen, U., Wilson, I.A. Structural evidence for induced fit as a mechanism for antibody-antigen recognition. Science 255:959-965, 1992.
5. Stanfield, R.L., Takimoto-Kamimura, M., Rini, J.M., Profy, A.T., Wilson, I.A. Major antigen-induced domain rearrangements in an antibody. Structure 1:83-93, 1993.
6. Smith, T.J., Chase, E.S., Schmidt, T.J., Olson, N.H., Baker, T.S. Neutralizing antibody to human rhinovirus 14 penetrates the receptor-binding canyon. Nature 383:350-354, 1996.
7. Banfield, M.J., King, D.J., Mountain, A., Brady, R.L. Crystal structure of the Fab fragment of a monoclonal antibody specific for Carcinoembryonic antigen. Acta Crystallogr. D52:1107-1113, 1996.
8. Lane, D.M., Eagle, K.F., Begent, R.H.J., Hope-Stone, L.D., Green, A.J., Casey, J.L., Keep, P.A., Kelly, A.M.B., Lederman, J.A., Glaser, M.G., Hilson A.J.W. Radioimmunotherapy of metastatic colorectal tumours with iodine-131-labelled antibody to carcinoembryonic antigen: phase I/II study with comparative biodistribution of intact and F(ab′)2 antibodies. Br. J. Cancer 70:521-525, 1994.
9. Adair, J.R., Athwal, D.S., Emtage J.S. Humanised Antibodies. Patent Application, WO91/09967, 1991.
10. Adair, J.R., Bodmer, M.W., Mountain, A., Owens, R.J. CDR-grafted anti-CEA antibodies and their production. Patent Application, WO 92/01059, 1992.
11. Zotter, S., Hageman, P.C., Lossnitzer, A., Mooi, W.J., Hilgers, J. Tissue and tumor distribution of human polymorphic epithelial mucin. Cancer Rev. 11-12:55-101, 1988.
12. Baker, T.S., Bose, C.C., Caskey-Finney, H.M., King, D.J., Lawson, A.D.G., Lyons, A., Mountain, A., Owens, R.J., Rolfe, M.R., Sehdev, M., Yarranton, G.T., Adair, J.R. Humanization of an anti-mucin antibody for breast and ovarian cancer therapy. In: "Antigen and Antibody Molecular Engineering in Breast Cancer Diagnosis and Treatment." Ceriani, R.L. (ed.). New York: Plenum, 1994:61-82.
13. van Hof, A.C., Molthoff, C.F.M., Davies, Q., Perkins, A.C., Verheijen, R.H.M., Kenemans, P., den Hollander, W., Willhelm, A.J., Baker, T.S., Sopwith, M., Frier, M., Symonds, M., Roos, J.C. Biodistribution of 111-Indium labelled engineered human antibody CTM01 in ovarian cancer patients: influence of protein dose. Cancer Res. 56:5179-5185, 1996.
14. Hinman, L.M., Hamann, P.R., Wallace, R., Menendez, A.T., Durr, F.E., Upeslacis, J. Preparation and characterization of monoclonal antibody conjugates of the calicheamicins: a novel and potent family of antitumor antibiotics. Cancer Res. 53:3336-3342, 1993.
15. King, D.J., Adair, J.R., Angel, S., Lowe, D.C., Proudfoot, K.A., Lloyd, J.C., Bodmer, M.W., Yarranton, G.T. Expression, purification and characterisation of a mouse-human chimeric antibody and chimeric Fab' fragment. Biochem. J. 281:317-323, 1992.
16. King, D.J., Antoniw, P., Owens, R.J., Adair, J.R., Haines, A.M.R., Farnsworth, A.P.H., Finney, H., Lawson, A.D.G., Lyons, A., Baker, T.S., Baldock, D., Mackintosh, J., Gofton, C., Yarranton, G.T., McWilliams, W., Shochat, D., Leichner, P.K., Welt, S., Old, L.J., Mountain, A. Preparation and preclinical evaluation of humanised A33 immunoconjugates for radioimmunotherapy. Br. J. Cancer 72:1364-1372, 1995.
17. Otwinowski, Z. DENZO and SCALEPACK: data processing and scaling programmes. Proceedings of the Daresbury study weekend 1993.
18. Navaza, J. AMoRe: an automated package for molecular replacement. Acta Crystallogr. A50:157-163, 1994.
19. Brunger, A.T. XPLOR Manual version 3.1. 1992.
20. Collaborative Computing Project Number 4. Programs for protein crystallography. Acta Crystallogr. D50:760-763, 1994.
21. Brunger, A.T. The free R value: a novel statistical quantity for assessing the accuracy of crystal structures. Nature 355:472-474, 1992.
22. Jones, T.A., Zou, J.-Y, Cowan, S.W., Kjeldgaard, M. Improved methods for building protein models in electron-density maps and the location of errors in these models. Acta Crystallogr. A, 47:110-119, 1991.
23. Jiang, J.-S., Brunger, A.T. Protein hydration observed by x-ray diffraction: solvation properties of penicillopepsion and neuraminidase crystal structures, J. Mol. Biol. 243:100-115, 1994.
24. Read, R.J. Improved Fourier coefficients for maps using phases from partial structures with errors. Acta Crystallogr. A42:140-149, 1986.
25. Ramachandran, N.G., Sasisekharen, V., Conformation of polypeptides and proteins. Adv. Protein Chem. 23:283-437, 1968.
26. Chothia, C., Lesk, A.M., Tramontano, A., Levitt, M., Smith-Gill, S.J., Air, G., Sheriff, S., Padlan, E.A., Davies, D., Tulip, W.R., Colman, P.M., Spinelli, S., Alzari, P.M., Poljak, R.J. Conformations of immunoglobulins hypervariable regions. Nature 342:877-883, 1989.
27. H residues 59 to 65. Proteins 18:49-62, 1994.
28. Holmes, M.A., Foote, J. Structural consequences of humanizing an antibody. J. Immunol. 158:2192-2201, 1997.
29. Kabat, E.A., Wu, T.T., Reid-Miller, M., Perry, H.M., Gottesman, K.S. "Sequences of Proteins of Immunological Interest," 4th Ed. Bethesda, MD: National Institutes of Health, 1987.