Synthesis of green fluorescent protein-ricin and monitoring of its intracellular trafficking

Bioconjugate Chemistry

Volumn 8, Issue 5, 1997, Pages 743-750

  Tagge, Edward ^a   Harris, Billie ^a   Burbage, Chris ^a   Hall, Philip ^b   Vesely, Joseph ^a   Willingham, Mark ^a   Frankel, Arthur ^a,b  

^a MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^b MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CELL MEMBRANES; CYTOLOGY; ESCHERICHIA COLI; GENETIC ENGINEERING; MAMMALS; PROTEINS; PURIFICATION;

CELL-BE; CELL/B.E; CELL/BE; ENHANCED GREEN FLUORESCENT PROTEIN; FUSION PROTEINS; GREEN FLUORESCENT PROTEIN; INTRACELLULAR TRAFFICKING; MAMMALIAN CELLS; N TERMINUS; SIZE EXCLUSION;

FLUORESCENCE;

ESCHERICHIA COLI; MAMMALIA;

EID: 0031239694     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc9700749     Document Type: Article

References (33)

1. Baenziger, J., and Fiete, D. (1979) Structural determinants of Ricinus communis agglutinin and toxin specificity for oligosaccharides. J. Biol. Chem. 254, 9795-9799.
2. Sandvig, K., and Olsnes, S. (1982) Entry of the toxic proteins abrin, modeccin, ricin and diphtheria toxin into cells. J. Biol. Chem. 257, 7504-7513.
3. Olsnes, S., Refsnes, K., and Pihl, A. (1974) Mechanism of action of the toxic lectins abrin and ricin. Nature 249, 627-631.
4. Ray, B., and Wu, H. (1982) Chinese hamster ovary cell mutants defective in the internalization of ricin. Mol. Cell. Biol. 2, 535-544.
5. van Deurs, B., Pedersen, L., Sundan, A., Olsnes, S., and Sandvig, K. (1985) Receptor-mediated endocytosis of ricin-colloidal gold conjugate in Vero Cells. Exp. Cell Res. 159, 287-304.
6. Nicholson, G. (1974) Ultrastructural analysis of toxin binding and entry into mammalian cells. Nature 251, 628-629.
7. Gonatas, J., Stieber, A., Olsnes, S., and Gonatas, N. (1980) Pathways involved in fluid phase and adsorbtive endocytosis in neuroblastoma. J. Cell Biol. 87, 579-588.
8. van Deurs, B., Tonnessen, T., Petersen, O., Sandvig, K., and Olsnes, S. (1986) Routing of internalized ricin and ricin conjugates to the Golgi complex. J. Cell Biol. 102, 37-47.
9. van Deurs, B., Petersen, O., Olsnes, S., and Sandvig, K. (1987) Delivery of internalized ricin from endosomes to cisternal Golgi elements is a discontinuous, temperature-sensitive process. Exp. Cell Res. 171, 137-152.
10. Sandvig, K., Prydz, K., Hansen, S., and van Deurs, B. (1991) Ricin transport in Brefeldin A-treated cells: correlation between Golgi structure and toxic effect. J. Cell Biol. 115, 971-981.
11. Wales, R., Chaddock, J., Roberts, L., and Lord, J. (1992) Addition of an ER retention signal to the ricin A chain increases the cytotoxicity of the holotoxin. Exp. Cell Res. 203, 1-4.
12. Wales, R., Roberts, L., and Lord, J. (1993) Addition of an endoplasmic reticulum retrieval sequence to ricin A chain signficantly increases its cytotoxicity to mammalian cells. J. Biol. Chem. 268, 23986-23990.
13. Tagge, E., Chandler, J., Tang, B., Hong, W., Willingham, M., and Frankel, A. (1996) Cytotoxicity of KDEL-terminated ricin toxins correlates with distribution of the KDEL receptor in the Golgi. J. Histochem. Cytochem. 44, 159-165.
14. Simpson, J., Dascher, C., Roberts, L., Lord, J., and Balch, W. (1995) Ricin cytotoxicity is sensitive to recycling between the endoplasmic reticulum and the Golgi complex. J. Biol. Chem. 270, 20078-20083.
15. Masuho, Y., Kishida, K., Saito, M., Umemoto, N., and Hara, T. (1982) Importance of the antigen-binding valency and the nature of the cross-linking bond in ricin A-chain conjugates with antibody. J. Biochem. 91, 1583-1591.
16. Argent, R., Roberts, L., Wales, R., Robertus, J., and Lord, J. (1994) Introduction of a disulfide bond into ricin A chain decreases the cytotoxicity of the ricin holotoxin. J. Biol. Chem. 269, 26705-26710.
17. Szewczak, A., Moore, P., Chang, Y., and Wool, I. (1993) The conformation of the sarcin/ricin loop from 28S ribosomal RNA. Proc. Natl. Acad. Sci. U.S.A. 90, 9581-9585.
18. Press, O., Vitetta, E., Farr, A., Hansen, J., and Martin, P. (1986) Evaluation of ricin A-chain immunotoxins directed against human T cells. Cell. Immunol. 102, 10-20.
19. Oosterhout, Y., Preijers, F., Wessels, H., and Witte, T. (1992) Cytotoxicity of CD3-ricin A chain immunotoxins in relation to cellular uptake and degradation kinetics. Cancer Res. 52, 5921-5925.
20. Manske, J., Buchsbaum, D., and Vallera, D. (1989) The role of ricin B chain in the intracellular trafficking of anti-CD5 immuntoxins. J. Immunol. 142, 1755-1766.
21. Prasher, D. (1995) Using GFP to see the light. Trends Genet. 11, 320-323.
22. Chalfie, M. (1995) Green fluorescent protein. Photochem. Photobiol. 62, 651-656.
23. Ormo, M., Cubitt, A., Kallio, K., Gross, L., Tsien, R., Remington, S. (1996) Crystal structure of the Aequorea victoria green fluorescent protein. Science 273, 1392-1395.
24. Cormack, B., Valdivia, R., and Falkow, S. (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173, 33-38.
25. Ludin, B., Doll, T., Meili, R., Kaech, S., and Matus, A. (1996) Application of novel vectors for GFP-tagging of proteins to study microtubule-associated proteins. Gene 173, 107-111.
26. De Giorgi, F., Brini, M., Bastianutto, C., Marsault, R., Montero, M., Pizzo, P., Rossi, R., Rizzuto, R. (1996) Targeting aequorin and green fluorescent protein to intracellular organelles. Gene 173, 113-117.
27. Frankel, A., Burbage, C., Fu, T., Tagge, E., Chandler, J., and Willingham, M. (1996) Ricin toxin contains at least three galactose-binding sites located in B chain subdomains 1α, 1β, and 2γ. Biochemistry 35, 14747-14756.
28. Heim, R., Prasher, D. C., and Tsien, R. Y. (1994) Wavelength mutations and postranslational autooxidation of green fluorescent protein. Proc. Natl. Acad. Sci. U.S.A. 91, 12501-12504.
29. Olsnes, S., and Pihl, A. (1973) Different biological properties of the two constituent peptide chains of ricin, a toxic protein inhibiting protein synthesis. Biochemistry 12, 3121-3126.
30. Bushuev, V., and Tonevitsky, A. (1989) High mobility of N-terminal parts of A and B subunits of ricin. J. Biomol. Struct. Dynamics 6, 1061-1070.
31. Rutenber, E., and Robertus, J. (1991) Structure of ricin B-chain at 2.5 Ångstrom resolution. Proteins 10, 260-269.
32. Frankel, A. E., Roberts, H., Afrin, L., Vesely, J., and Willingham, M. C. (1994) Expression of ricin B chain in Spodoptera frugiperda. Biochem. J. 303, 787-794.
33. Engert, A., Brown, A., and Thorpe, P. (1991) Resistance of myeloid leukemia cell lines to ricin A-chain immunotoxins. Leuk. Res. 15, 1079-1086.