CFTR activation: Additive effects of stimulatory and inhibitory phosphorylation sites in the R domain

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 273, Issue 1 17-1, 1997, Pages

  Wilkinson, Daniel J ^b   Strong, Theresa V ^b   Mansoura, Monique K ^b   Wood, Deborah L ^b   Smith, Stephen S ^b   Collins, Francis S ^b   Dawson, David C ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b NONE

Author keywords

Adenosine 3',5' cyclic monophosphate dependent activation; Chloride channels; Consensus; Cystic fibrosis transmembrane conductance regulator; Mutagenesis; Protein kinaseA; Serines; Xenopus oocytes

Indexed keywords

AMINO ACID; CYCLIC AMP DEPENDENT PROTEIN KINASE; TRANSMEMBRANE CONDUCTANCE REGULATOR;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; CHANNEL GATING; CYSTIC FIBROSIS; FEMALE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PHOSPHORYLATION; XENOPUS LAEVIS;

EID: 0030816586     PISSN: 10400605     EISSN: None     Source Type: Journal    
DOI: 10.1152/ajplung.1997.273.1.l127     Document Type: Article

References (21)

1. Anderson, M. P., H. A. Berger, D. P. Rich, R. J. Gregory, A. E. Smith, and M. J. Welsh. Nucleoside triphosphates are required to open the CFTR chloride channel. Cell 67: 775-784, 1991.
2. Bear, C. E., C. Li, N. Kartner, R. J. Bridges, T. J. Jensen, M. Ramjeesingh, and J. R. Riordan. Purification and functional reconstitution of the cystic fibrosis transmembrane conductance regulator (CFTR). Cell 68: 809-818, 1992.
3. Becq, F., T. J. Jensen, X.-B Chang, A. Savoia, J. M. Rommens, L.-C. Tsui, M. Buchwald, J. R. Riordan and J. W. Hanrahan. Phosphatase inhibitors activate normal and defective CFTR chloride channels. Proc. Natl. Acad. Sci. USA 91: 9160-9164, 1994.
4. Berger, H. A., M. P. Anderson, R. J. Gregory, S. Thompson, P. W. Howard, R. A. Maurer, R. Mulligan, A. E. Smith, and M. J. Welsh. Identification and regulation of the cystic fibrosis transmembrane conductance regulator-generated chloride channel. J. Clin. Invest. 88: 1422-1431, 1991.
5. Chang, X.-B., J. A. Tabcharani, Y.-X. Hou, T. J. Jensen, N. Kartner, N. Alon, J. W. Hanrahan, and J. R. Riordan. Protein kinase A (PKA) still activates CFTR chloride channel after mutagenesis of all 10 PKA consensus phosphorylation sites. J. Biol. Chem. 268: 11304-11311, 1993.
6. Cheng, S. H., D. P. Rich, J. Marshall, R. J. Gregory, M. J. Welsh, and A. E. Smith. Phosphorylation of the R domain by cAMP-dependent protein kinase regulates the CFTR chloride channel. Cell 66: 1027-1036, 1991.
7. Dawson, D. C., M. K. Mansoura, S. S. Smith, and D. J. Wilkinson. CFTR: molecular basis for Cl channel function. In: Cystic Fibrosis-Current Topics, edited by J. A. Dodge, D. J. H. Brock, and J. H. Widdicombe. Chichester, UK: Wiley, 1996, vol. 3, p. 39-64.
8. Drumm, M. L., D. J. Wilkinson, L. S. Smit, R. T. Worrell, T. V. Strong, R. A. Frizzell, D. C. Dawson, and F. S. Collins. Chloride conductance expressed by F508 and other mutant CFTRs in Xenopus oocytes. Science 254: 1797-1799, 1991.
9. Gadsby, D. C., and A. C. Nairn. Regulation of CFTR channel gating. Trends Biochem. Sci. 19: 513-518, 1994.
10. Gadsby, D. C., and A. C. Nairn. Regulation of CFTR channel activity. In: Cystic Fibrosis-Current Topics, edited by J. A. Dodge, D. J. H. Brock, and J. H. Weddicombe. Chichester, UK: Wiley, 1996, vol. 3, p. 65-90.
11. - conductance in mammalian heart. J. Gen. Physiol. 101: 629-650, 1993.
12. Hwang, T. R., G. Nagel, A. C. Nairn, and D. C. Gadsby. Regulation of the gating of cystic fibrosis transmembrane conductance regulator Cl channels by phosphorylation and ATP hydrolysis. Proc. Natl. Acad. Sci. USA 91: 4698-4702, 1994.
13. Picciotto, M. R., J. A. Cohn, G. Bertuzzi, P. Greengard, and A. C. Nairn. Phosphorylation of the cystic fibrosis transmembrane conductance regulator. J. Biol. Chem. 267: 12742-12752, 1992.
14. - channel by negative charge in the R domain. J. Biol. Chem. 268: 20259-20267, 1993.
15. Rich, D. P., R. J. Gregory, M. P. Anderson, P. Manavalan, A. E. Smith, and M. J. Welsh. Effect of deleting the R Domain on CFTR-generated chloride channels. Science 253: 205-297, 1991.
16. Riordan, J. R., J. M. Rommens, B.-S. Kerem, N. Alon, R. Rozmahel, Z. Grzelczak, J. Zielenski, S. Lok, N. Plavsic, J.-L. Chou, M. L. Drumm, M. C. Iannuzzi, F. S. Collins, and L.-C. Tsui. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989.
17. Smit, L. S., D. J. Wilkinson, M. K. Mansoura, F. S. Collins, and D. C. Dawson. Functional roles of the nucleotide-binding folds in the activation of the cystic fibrosis transmembrane conductance regulator. Proc. Natl. Acad. Sci. USA 90: 9963-9967, 1993.
18. - channel in CHO cells stably expressing the cystic fibrosis gene. Nature 352: 628-631, 1991.
19. Townsend, R. R., P. H. Lipnionas, B. M. Tulk, and A. S. Verkman. Identification of protein kinase-A phosphorylation sites on NBD1-domains and R-domains of CFTR using electro-spray mass spectrometry with selective phosphate ion monitoring. Protein Sci. 5: 1865-1873, 1996.
20. Wilkinson, D. J., M. K. Mansoura, P. Y. Watson, L. S. Smit, F. S. Collins, and D. C. Dawson. CFTR: the nucleotide binding folds regulate the accessibility and stability of the active state. J. Gen. Physiol. 107: 103-119, 1996.
21. Wilkinson, D. J., T. V. Strong, F. S. Collins, and D. C. Dawson. CFTR activation: stimulatory and inhibitory phosphorylation sites in the R domain (Abstract). Pediatr. Pulmonol. S10: 179, 1994.