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of special interest. Ras could not be shown to activate c-Raf-1 in vitro, suggesting that Ras simply acted as a shuttle vehicle for moving Raf to the plasma membrane where it could be activated by other mechanisms. These authors, however, demonstrated that Ras could do far more by showing that GTP-Ras directly activated B-Raf in a pure cell-free system.
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of special interest. By expressing effector loop mutants of V12 Ras in cells, these authors demonstrated that Ras is a common upstream element of at least two distinct signaling pathways. One is the classic MAPK pathway and the other is pathway that regulates the actin cytoskeleton; both appear to be required for the optimal mitogenic signal from activated Ras. (This work is an application of an approach pioneered by Michael White and Mike Wigler.)
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Diaz-Meco, M.T.1
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Kuriyama M, Harada N, Kuroda S, Yamamoto T, Nakafuku M, Iwamatsu A, Yamamoto D, Prasad R, Croce C, Canaani E, et al. Identification of AF-6 and Canoe as putative targets for Ras. J Biol Chem. 271:1996;607-610.
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Cloning of the ALL-1 fusion partner, the AF-6 gene, involved in acute myeloid leukemias with the t(6;11) chromosome translocation
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A human protein selected for interference with Ras functions interacts directly with Ras and competes with Raf
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of special interest. This is a validation of earlier work from Colicelli using suppression of Ras function in yeast by expressed human cDNAs as a screen for proteins that bind to activated Ras at the effector site. The authors also point out the conserved nature of the Ras pathway among eukaryotes and how this can be exploited to identify novel effectors.
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Wolthius RMF, Bauer B, Van't Veer LJ, De Vries-Smits AMM, Cool RH, Spaargaren M, Wittinghofer A, Burgering BMT, Bos JL. RalGDS-like factor (Rlf) is a novel Ras and Rap 1A-associating protein. Oncogene. 13:1996;353-362.
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of outstanding interest. Brilliant demonstration of the power of effector mutants to dissect Ras signaling pathways. This approach has been invaluable for the Ras field and is now being applied equally successfully to dissection of multiple Rac signaling pathways.
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White MA, Nicolette C, Minden A, Polverino A, Van Aelst L, Karin M, Wigler MH. Multiple ras functions can contribute to mammalian cell transformation. of outstanding interest Cell. 80:1995;533-541 Brilliant demonstration of the power of effector mutants to dissect Ras signaling pathways. This approach has been invaluable for the Ras field and is now being applied equally successfully to dissection of multiple Rac signaling pathways.
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