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Volumn 275, Issue 5302, 1997, Pages 977-979

Immunoglobulin E production in the absence of interleukin-4-secreting CD1-dependent cells

Author keywords

[No Author keywords available]

Indexed keywords

CD1 ANTIGEN; IMMUNOGLOBULIN D ANTIBODY; IMMUNOGLOBULIN E; INTERLEUKIN 4; MESSENGER RNA;

EID: 0031028170     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5302.977     Document Type: Article
Times cited : (441)

References (33)
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    • note
    • 5 thymocytes from the indicated mice in 96-well microcultures. After 24 hours, supernatants were harvested, and IL-2 production was quantitated by HT-2 cell proliferation. Proliferation was converted to IL-2 units with the use of a standard curve generated with recombinant IL-2 (Genzyme).
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    • note
    • -/loW thymocytes were obtained by treatment of total thymocytes with antibody to HSA (clone J11d2) plus rabbit complement (Cederlane, Hornby, Ontario, Canada) for 45 min at 37°C, followed by isolation of viable cells on lympholyte M gradients (Cederlane). After Fc receptors were blocked by treatment with mAb 24G2, cells were stained with phycoerythrin-conjugated mAb specific for VβS.8.1/8.2 and fluorescein-conjugated mAb specific for NK1.1 or CD44, and then analyzed by flow cytometry. All mAbs were purchased from Pharmingen.
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    • note
    • -/low thymocytes in 1 ml of media. After 48 hours, supernatants were harvested and assayed for cytokine production (2). Recombinant IL-4 and IFN-γ (Genzyme) were used to generate standard curves.
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    • note
    • 2M-deficient BALB/c mice, T. Laufer for help with intravenous injections, and K. Sahmel for technical assistance. Supported by a grant from NIH to M.J.G. (RO1 AI40171) and a gift from the Mathers Foundation.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.