Somatostatin receptors in Neuro2A neuroblastoma cells: Operational characteristics

British Journal of Pharmacology

Volumn 120, Issue 1, 1997, Pages 45-51

  Koenig, J A ^a   Edwardson, J M ^a   Humphrey, P P A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

Author keywords

125I BIM 23027; Adenylyl cyclase inhibition; Ligand binding; Neuro2A cells; Somatostatin receptors

Indexed keywords

BIM 23027; CYCLIC AMP; GUANOSINE TRIPHOSPHATE; LIGAND; PERTUSSIS TOXIN; RADIOACTIVE IODINE; SODIUM CHLORIDE; SOMATOSTATIN; SOMATOSTATIN RECEPTOR; CITRININ; DEOXYGLUCOSE; GUANINE NUCLEOTIDE BINDING PROTEIN; IODINE 125;

ANIMAL CELL; ARTICLE; LIGAND BINDING; MOUSE; NEUROBLASTOMA CELL; NONHUMAN; PRIORITY JOURNAL; RECEPTOR AFFINITY; INTERNALIZATION;

ADENOSINE TRIPHOSPHATE; ANIMALS; BRAIN NEOPLASMS; CALCIUM; CELL MEMBRANE; CYCLIC AMP; GUANOSINE TRIPHOSPHATE; KINETICS; LIGANDS; MICE; NEUROBLASTOMA; PEPTIDES, CYCLIC; RECEPTORS, SOMATOSTATIN; SECOND MESSENGER SYSTEMS; SOMATOSTATIN; TUMOR CELLS, CULTURED; GTP-BINDING PROTEINS; IODINE RADIOISOTOPES;

EID: 0031027773     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0700858     Document Type: Article

References (37)

1. 2+ mobilization by cloned human somatostatin receptor subtypes 1-5, in transfected COS-7 cells. FEBS Lett., 348, 192-196.
2. BRADFORD, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72, 248-254.
3. BUSCAIL, L., ESTEVE, J.-P., SAINT-LAURENT, N., BERTRAND, V., REISINE, T., O'CARROLL, A.-M., BELL, G.I., SCHALLY, A.V., VAYSSE, N. & SUSINI, C. (1995). Inhibition of cell proliferation by the somatostatin analogue RC-160 is mediated by somatostatin receptor subtypes SSTR2 and SSTR5 through different mechanisms. Proc. Natl. Acad. Sci. U.S.A., 92, 1580-1584.
4. 2, in mouse fibroblast (Ltk-) cells. Br. J. Pharmacol., 117, 639-645.
5. GARCIA, P.D. & MYERS, R.M. (1994). Pituitary cell line GH3 express two somatostatin receptor subtypes that inhibit adenylyl cyclase: functional expression of rat somatostatin receptor subtypes 1 and 2 in human embryonic kidney cells. Mol. Pharmacol., 45, 402-409.
6. HERSHBERGER, R.E., NEWMAN, B.A., FLORIO, T., BUNZOW, J., CIVELLI, O., LI, X.-J., FORTE, M., STORK, P.J.S. (1994). The somatostatin receptors SSTR1 and SSTR2 are coupled to inhibition of adenylyl cyclase in chinese hamster ovary cells via pertussis toxin-sensitive pathways. Endocrinology, 134, 1277-1285.
7. 3]-octreotide by mouse and human pituitary tumor cells: increase by unlabelled octreotide. Endocrinology, 136, 3698-3706.
8. 125I]-BIM-23027. Neuropharmacology, 35, 1109-1120.
9. HOYER, D., BELL, G.I., BERELOWITZ, M., EPELBAUM, J., FENIUK, W., HUMPHREY, P.P.A., O'CARROLL, A.M., PATEL, Y.C., SCHONBRUNN, A., TAYLOR, J.E. & REISINE, T. (1995). Classification and nomenclature of somatostatin receptors. Trends Pharmacol. Sci., 16, 81-116.
10. HOYER, D., LUBBERT, H. & BRUNS, C. (1994). Molecular pharmacology of somatostatin receptors. Naunyn-Schmiedeberg's Arch. Pharmacol., 350, 441-453.
11. KLEUSS, C., HESCHELER, J., EWEL, C., ROSENTHAL, W., SCHULTZ, G. & WITTIG, B. (1991). Assignment of G-protein subtypes to specific receptors inducing inhibition of calcium currents. Nature, 353, 43-48.
12. KOENIG, J.A., EDWARDSON, J.M. & HUMPHREY, P.P.A. (1996). Somatostatin receptors in neuro2A neuroblastoma cells: ligand internalization. Br. J. Pharmacol., 120, 52-59.
13. LIU, Y.F., JAKOBS, K.H., RASENICK, M.M. & ALBERT, P.R. (1994). G protein specificity in receptor-effector coupling. J. Biol. Chem., 269, 13880-13886.
14. MAHY, N., WOOLAKLIS, M., MANNING, D. & REISINE, T. (1988). Characteristics of somatostatin desensitization in the pituitary tumor cell line AtT-20. J. Pharmacol. Exp. Ther., 24, 390-396.
15. MARIN, P., DELUMEAU, J.C., TENCE, M., CORDIER, J., GLOWINSKI, J. & PREMONT, J. (1991). Somatostatin potentiates the α1-adrenergic activation of phospholipase C in striatal astrocytes through a mechanism involving arachidonic acid and glutamate. Proc. Natl. Acad. Sci. U.S.A., 88, 9016-9020.
16. MCKEEN, E., FENIUK, W., MICHEL, A.D., KIDD, A.J. & HUMPHREY, P.P.A. (1996). Identification and characterisation of heterogenous somatostatin binding sites in rat distal colonic mucosa. Naunyn-Schmiedeberg's Arch. Pharmacol., 354, 543-549.
17. 2+ current modulated by cGMP-dependent protein kinase. Nature, 369, 337-339.
18. MUNSON, P.J. & RODBARD, D. (1980). LIGAND: a versatile computerised approach for characterization of ligand binding systems. Anal. Biochem., 107, 220-239.
19. O'CARROLL, A.-M., RAYNOR, K., LOLAIT, S.J. & REISINE, T. (1994). Characterization of cloned human somatostatin receptor SSTR5. Mol. Pharmacol., 46, 291-298.
20. 2+ mobilization between bradykinin and agonists for pertussis toxin-sensitive G-protein-coupled receptors in NG108-15 cells. FEBS Lett, 301, 223-226.
21. PATEL, Y.C., GREENWOOD, M.T., WARSZYNSKA, A., PANETTA, R. & SRIKANT, C.B. (1994). All five cloned human somatostatin receptors (hSSTR1-5) are functionally coupled to adenylyl cyclase. Biochem. Biophys. Res. Commun., 198, 605-612.
22. PATEL, Y.C. & SRIKANT, C.B. (1994). Subtype selectivity of peptide analogs for all five cloned human somatostatin receptors (hsstr1-5). Endocrinology, 135, 2814-2817.
23. 11]-somatostatin yields a super high affinity ligand for somatostatin receptors in GH4C1 pituitary cells. Mol. Pharmacol., 34, 651-658.
24. PRINZ, C., SACHS, G., WALSH, J.H., COY, D.H. & WU, S.V. (1994). The somatostatin receptor subtype on rat enterochromaffin-like cells. Gastroenterology, 107, 1067-1074.
25. RAYNOR, K., MURPHY, W.A., COY, D.H., TAYLOR, J.E., MOREAU, J.P., YASUDA, K., BELL, G.I. & REISINE, T. (1993a). Cloned somatostatin receptors: identification of subtype-selective peptides and demonstration of high affinity binding of linear peptides. Mol. Pharmacol., 44, 838-844.
26. RAYNOR, K., O'CARROLL, A.M., KONG, H., YASUDA, K., MAHAN, L.C., BELL, G.I. & REISINE, T. (1993b). Characterization of cloned somatostatin receptors SSTR4 and SSTR5. Mol. Pharmacol., 44, 385-392.
27. RAYNOR, K. & REISINE, T. (1992). Differential coupling of somatostatin-1 receptors to adenylyl cyclase in the rat striaturm vs. the pituitary and other regions of the rat brain. J. Pharmacol. Exp. Ther., 260, 841-848.
28. REISINE, T., KONG, H., RAYNOR, K., YANO, H., TAKEDA, J., YASUDA, K. & BELL, G.I. (1993). Splice variant of the somatostatin receptor 2 subtype, somatostatin receptor 2B couples to adenylyl cyclase. Mol. Pharmacol., 44, 1008-1015.
29. 2(a) receptor in the rat brain and spinal cord. Neuroscience, (in press).
30. SCHWEITZER, P., MADAMBA, S. & SIGGINS, G.R. (1990). Arachidonic acid metabolites as mediators of somatostatin-induced increase of neuronal M-current. Nature, 346, 464-467.
31. TAYLOR, J.E., THEVENIAU, M.A., BASHIRZADEH, R., REISINE, T. & EDEN, P.A. (1994). Detection of somatostatin receptor subtype 2 (SSTR2) in established tumors and tumor cell lines: evidence for SSTR2 heterogeneity. Peptides, 15, 1229-1236.
32. TRAINA, G., CANNISTRATO, S. & BAGNOLI, P. (1996). Effects of somatostatin on intracellular calcium concentration in PC12 cells. J. Neurochem., 66, 485-492.
33. VANETTI, M., VOGT, G. & HOLLT, V. (1993). The two isoforms of the mouse somatostatin receptor (mSSTR2A and mSSTR2B) differ in coupling efficiency to adenylate cyclase and in agonist-induced receptor desensitisation. FEBS Lett., 331 260-266.
34. + channels through protein dephosphorylation. Nature, 351, 570-572.
35. WILKINSON, G.F., THURLOW, R.J., SELLERS, L.A., COOTE, J.E., FENIUK, W. & HUMPHREY, P.P.A. (1996). Potent antagonism by BIM-23056 at the human recombinant somatostatin sst5 receptor. Br. J. Pharmacol., 118, 445-447.
36. WILLIAMS, A.J., MICHEL, A.D., COOTE, J.E., SELLERS, L., FENIUK, W. & HUMPHREY, P.P.A. (1996). Characterisation of the human recombinant sst5 receptor in CHO-K1 cells by quantification of guanosine-5′-O-(3-[35]-thio)triphosphate binding. Br. J. Pharmacol., 117, 10P.
37. WU-WONG, J.R., CHIOU, W.J., MAGNUSON, S.R. & OPGENORTH, T.J. (1995). Endothelin receptor in human astrocytoma U373MG cells: binding, dissociation, receptor internalization. J. Pharmacol. Exp. Ther., 274, 499-507.