The dnrM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene cluster

Microbiology

Volumn 142, Issue 2, 1996, Pages 269-275

  Gallo, Mark A ^a   Ward, Joanne ^a,b   Hutchinson, C R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b Sandoz Clinical Development Centre   (United Kingdom)

Author keywords

Daunosamine; Deoxyamino sugar; Glucose 1 phosphate thymidylyl transferase; Inactive gene; TDP D glucose 4,6 dehydratase

Indexed keywords

DAUNORUBICIN; DAUNOSAMINE; HYDROLYASE; TRANSFERASE;

ARTICLE; CARBOHYDRATE SYNTHESIS; DNA SEQUENCE; FRAMESHIFT MUTATION; GENE CLUSTER; GENE LOCUS; GENE MUTATION; GENE SEQUENCE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA TRANSLATION; STREPTOMYCES;

STREPTOMYCES PEUCETIUS;

EID: 0030060592     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/13500872-142-2-269     Document Type: Article

References (35)

1. Arcamone, F. (1981). Doxorubicin. Med Chem Ser Monogr 17, 25-31.
2. Arcamone, F., Cassinelli, G., Fantini, G., Grein, A., Orezzi, P., Pol, C. & Spalla, C (1969). 14-hydroxydaunomycin, a new antitumor antibiotic from Streptomyces peucetius var. caesius. Biotechnol Bioeng 11, 1101-1110.
3. Chater, K. F. & Bibb, M. J. (1995). Regulation of bacterial antibiotic production. In Products of Secondary Metabolism. Biotechnology, vol. 7. Weinheim: VCH.
4. Dekleva, M. L., Titus, J. A. & Strohl, W. R. (1985). Nutrient effects on anthracycline production by Streptomyces peucetius in a defined medium. Can J Microbiol 31, 287-294.
5. Devereux, J., Haeberli, P. & Smithies, O. (1984). A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12, 387-395.
6. Donadio, S. & Katz, L. (1992). Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea. Gene 110, 51-60.
7. Grimm, A., Madduri, K., Ali, A. & Hutchinson, C. R. (1994). Characterization of the Streptomyces peucetius ATCC 29050 genes encoding doxorubicin polyketide synthase. Gene 151, 1-10.
8. Guilfoile, P. G. & Hutchinson, C. R. (1991). A bacterial analog of the mdr gene of mammalian tumor cells is present in Streptomyces peucetius, the producer of daunorubicin and doxorubicin. Proc Natl Acad Sci USA 88, 8553-8557.
9. Hillemann, D., Puhler, A. & Wohlleben, W. (1991). Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. Nucleic Acids Res 19, 727-731.
10. Hopwood, D. A. & Sherman, D. H. (1990). Molecular genetics of polyketides and its comparison to fatty acid biosynthesis. Annu Rev Genet 24, 37-66.
11. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schrempf, H. (1985). Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: The John Innes Foundation.
12. Hutchinson, C. R. & Fujii, I. (1995). Polyketide synthase gene manipulation: a structure-function approach in engineering novel antibiotics. Annu Rev Microbiol 49, 201-38.
13. Katz, L. & Donadio, S. (1993). Polyketide synthesis: prospects for hybrid antibiotics. Annu Rev Microbiol 47, 875-912.
14. Krugel, H., Schumann, G., Hanel, F. & Fielder, G. (1993). Nucleotide sequence analysis of five putative Streptomyces griseus genes, one of which complements an early function in daunorubicin biosynthesis that is linked to a putative gene cluster involved in TDP-daunosamine formation. Mol & Gen Genet 241, 326-333.
15. Linton, K. J., Jarvis, B. W. & Hutchinson, C. R. (1995). Cloning of the genes encoding thymidine diphosphoglucose 4,6-dehydratase and thymidine diphospho-4-keto-6-deoxyglucose 3,5-epimerase from the erythromycin-producing Saccharopolyspora erythraea. Gene 153, 33-40.
16. Liu, H. W. & Thorson, J. S. (1994). Pathways and mechanisms in the biogenesis of novel deoxy sugars by bacteria. Annu Rev Microbiol 48, 223-256.
17. MacNeil, D. J., Occi, J. L., Gewain, K. M. & MacNeil, T. (1994). Correlation of the avermectin polyketide synthase genes to the avermectin structure: implications for designing novel avermectins. Ann NY Acad Sci 721, 123-132.
18. Madduri, K. & Hutchinson, C. R. (1995a). Functional characterization and transcriptional analysis of the dnrR1 locus, which controls daunorubicin biosynthesis in Streptomyces peucetius. J Bacteriol 177, 1208-1215.
19. Madduri, K. & Hutchinson, C. R. (1995b). Functional characterization and transcriptional analysis of a gene cluster governing early and late steps in daunorubicin biosynthesis in Streptomyces peucetius. J Bacteriol 177, 3879-3884.
20. Merson-Davies, L. A. & Cundliffe, E. (1994). Analysis of five tylosin biosynthetic genes from the tylIBA region of the Streptomyces fradiae genome. Mol Microbiol 13, 349-355.
21. Otten, S. L., Stutzman-Engwall, K. J. & Hutchinson, C. R. (1990). Cloning and expression of daunorubicin biosynthesis genes from Streptomyces peucetius and S. peucetius subsp. caesius. J Bacteriol 172, 3427-3434.
22. Otten, S. L., Ferguson, J. & Hutchinson, C. R. (1995a). Regulation of daunorubicin production in Streptomyces peucetius by the dnrR2 locus. J Bacteriol 177, 1216-1224.
23. Otten, S. L., Liu, X., Ferguson, J. & Hutchinson, C. R. (1995b). Cloning and characterization of the Streptomyces peucetius dnrQS genes encoding a daunosamine biosynthesis enzyme and a glycosyl transferase involved in daunorubicin biosynthesis. J Bacteriol 177, 6688-6692.
24. Pissowotzki, K., Mansouri, K. & Piepersberg, W. (1991). Genetics of streptomycin production in Streptomyces griseus: molecular structure and putative function of genes strELMB2N. Mol & Gen Genet 231, 113-123.
25. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
26. Stevenson, G., Neal, B., Liu, D., Hobbs, M., Packer, N. H., Batley, M., Redmond, J. W., Lindquist, L. & Reeves, P. (1994). Structure of the O antigen of Escherichia coli K-12 and the sequence of its rjb gene cluster. J Bacteriol 176, 4144-4156.
27. Stutzman-Engwall, K. J. & Hutchinson, C. R. (1989). Multigene families for anthracycline antibiotic production in Streptomyces peucetius. Proc Natl Acad Sci USA 86, 3135-3139.
28. Stutzman-Engwall, K. J., Otten, S. L. & Hutchinson, C. R. (1992). Regulation of secondary metabolism in Streptomyces: its use for the overproduction of daunorubicin in Streptomyces peucetius. J Bacteriol 174, 144-154.
29. Summers, R. G., Wendt-Pienkowski, E., Motamedi, H. & Hutchinson, C. R. (1992). Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase. J Bacteriol 174, 1810-1820.
30. Thompson, M. W., Strohl, W. R. & Floss, H. G. (1992). Purification and characterization of TDP-D-glucose 4,6-dehydratase from anthracycline-producing streptomycetes. J Gen Microbiol 138, 779-786.
31. Thorson, J. S., Lo, S. F., Liu, H.-W. & Hutchinson, C. R. (1993). Biosynthesis of 3,6-dideoxy-hexoses: new mechanistic reflections upon 2,6-dideoxy, 4,6-dideoxy and amino sugar construction. J Am Chem Soc 115, 6993-6994.
32. Vara, J., Lewandowska-Skarbek, M., Wang, Y.-G., Donadio, S. & Hutchinson, C. R. (1989). Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythreus). J Bacteriol 171, 5872-5881.
33. Wright, F. & Bibb, M. J. (1992). Codon usage in the G+C-rich Streptomyces genome. Gene 113, 55-65.
34. Yanisch-Perron, C., Vieira, J. & Messing, J. (1985). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.
35. Yao, Z. & Valvano, M. A. (1994). Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W31100: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a. J Bacteriol 176, 4133-4143.