Biochemical requirements for the targeting and fusion of ER-derived transport vesicles with purified yeast Golgi membranes

Journal of Cell Biology

Volumn 132, Issue 3, 1996, Pages 277-289

  Lupashin, Vladimir V ^a,b   Hamamoto, Susan ^a   Schekman, Randy W ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE 5' O (3 THIOTRIPHOSPHATE); ADENOSINE TRIPHOSPHATE; ALPHA 1,6 MANNOSYLTRANSFERASE; CALCIUM; EGTAZIC ACID; ETHYLENE GLYCOL 1,2 BIS(2 AMINOPHENYL) ETHER N,N,N',N' TETRAACETIC ACID; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); GUANOSINE DIPHOSPHATASE; GUANOSINE TRIPHOSPHATE; MANNOSYLTRANSFERASE; MEMBRANE PROTEIN; PHOSPHATASE; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; GOLGI COMPLEX; HYDROLYSIS; MEMBRANE FUSION; MEMBRANE RECONSTITUTION; MEMBRANE TRANSPORT; MEMBRANE VESICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; SACCHAROMYCES CEREVISIAE; TEMPERATURE SENSITIVE MUTANT; YEAST;

BIOLOGICAL MARKERS; BIOLOGICAL TRANSPORT; CELL FRACTIONATION; ENDOPLASMIC RETICULUM; FUNGAL PROTEINS; GOLGI APPARATUS; INTRACELLULAR MEMBRANES; MANNOSYLTRANSFERASES; MEMBRANE FUSION; MICROSCOPY, ELECTRON; ORGANELLES; PYROPHOSPHATASES; SACCHAROMYCES CEREVISIAE;

EID: 0030062831     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.132.3.277     Document Type: Article

References (46)

1. Abeijon, C., P. Orlean, P. W. Robbins, and C. B Hirshberg. 1989 Topography of glycosylation in yeast: characterization of GDP-mannose transport and lumenal guanosine diphosphatase activities in Golgi-like vesicles. Proc Natl. Acad. Sci. USA 86:6935-6939
2. Achstetter, T , A. Franzusoff, C Field, and R. Schekman. 1988. SEC7 encodes an unusual, high molecular weight protein required for membrane traffic from the yeast Golgi apparatus. J Biol. Chem. 263:11711-11717.
3. Bacon, R A., A Salminen, H. Ruohola, P Novick, and S. Ferro-Novick. 1989. The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants. J. Cell Biol. 109:1015-1022.
4. Baker, D., L. Hicke, M. Rexach, M. Scheyer, and R. Schekman. 1988. Reconstitutionof SEC gene product-dependent intercompartmental protein transport. Cell. 54:335-344.
5. 2+ function independently in a cell-free protein transport reaction. Proc. Natl. Acad. Sci. USA 87:355-359.
6. Barlowe, C., L. Orci, T. Yeung, M. Hosobuchi, S. Hamamoto, N. Salama, M Rexach, M. Ravazzola, and R. Schekman. 1994. COPII. a membrane coat formed by Sec proteins that drive vesicle budding from the ER. Cell 77.895-907
7. Barroso, M., D. S. Nelson, and E. Sztul. 1995. Transcytosis-associated protein (TAP)/p115 is a general fusion factor required for binding of vesicles to acceptor membranes. Proc Natl. Acad. Sci. USA. 92:527-531.
8. Bowman, B. J., and C. W Slayman. 1979. The effects of vanadate on the plasma membrane ATPase of Neurospora crassa. J. Biol. Chem. 254.2928-2934.
9. Clary, D. O., I. C. Griff, and J. E. Rothman. 1990. SNAPS, a family of NSF attachment proteins involved in intracellular membrane fusion in animal and yeast. Cell 61:709-721
10. Dascher, C., R. Ossig, D. Gallwitz, and H. D. Schmitt. 1991. Identification and structure of four yeast genes (SLY) that are able to suppress the functional loss of YPTI, a member of the RAS superfamily Mol. Cell Biol. 11.872-885
11. Franzusoff, A., and R. Schekman 1989 Functional compartments of the yeast Golgi apparatus are defined by the sec7 mutation. EMBO J. 8:2695-2702.
12. Franzusoff, A., E. Lauze, and K. E. Howell. 1992. Immunoisolation of Sec7p-coated vesicles from the yeast secretory pathway. Nature (Lond.). 355:173-175.
13. 2+-dependent serine protease Proc. Natl. Acad. Sci. USA 86:1434-1438
14. Garrett, M. D., J. E. Zahner, C. M. Cheney, and P. J. Novick. 1994. GDI1 encodes a GDP dissociation inhibitor that plays an essential role in the yeast secretory pathway. EMBO J 13:1718-1728
15. Garcia, E. P., E. Gatti, M. Butler, J Burton, and P. De Camilli. 1994. A rat brain Sec1 homologue related to Rop and UNC18 interacts with syntaxin. Proc. Natl. Acad. Sci. USA 91:2003-2007
16. Graham, T. R., M. Seeger, G. S Payne, V. S. MacKay, and S. D. Emr. 1994. Clathrin-dependent localization of α1,3 mannosyltransferase to the Golgi complex of Saccharomyces cerevisiae. J. Cell Biol. 127:667-678.
17. Griff, I. C., R. W. Schekman, J. E. Rothman, and C. A. Kaiser. 1992. The yeast SEC17 gene product is functionally equivalent to mammalian αSNAP protein J. Biol. Chem. 267:12106-12115.
18. Hardwick, K. G., and H. R. B. Pelham. 1992. SED5 encodes a 39-kD integral membrane protein required for vesicular transport between ER and the Golgi complex. J Cell Biol. 119:513-521.
19. Hosobuchi, M., T. Kreis, and R. Schekman. 1992. SEC21 is a gene required for ER to Golgi protein transport that encodes a subunit of the yeast coatomer. Nature (Lond.) 360.603-605
20. Kaiser, C., and R. Schekman. 1990. Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway. Cell. 61:723-733.
21. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.). 227:680-685.
22. Lian, J. P , and S. Ferro-Novick. 1993. BosIp. an integral membrane protein of the Endoplasmic reticulum to Golgi transport vesicles, is required for their fusion competence. Cell. 73:735-745.
23. Mason, T. L., R. O. Poyton, D C. Wharton, and G. Schatz. 1973. Cytochrome c oxidase from baker's yeast. I. Isolation and properties. J. Biol. Chem 248: 1346-1354.
24. Nakajima, H., A. Hirata, Y. Ogawa, T. Yonehara, K Yoda, and M Yamasaki. 1991. A cytoskeleton-related gene, USO1, is required for intracellular protein transport in Saccharomyces cerevisiae. J. Cell Biol 113:245-260
25. Nakayama, K., T Nagasu, Y. Shimma, J. Kuromitsu, and Y. Jigami. 1992. OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine-linked oligosaccharides EMBO J. 11:2511-2519.
26. Novick, P., and P. Brennwald. 1993 Friends and family: the rope of the Rab GTPases in vesicular traffic. Cell. 75:597-601
27. Opheim, D. J. 1978. α-D-Mannosidase of Saccharomyces cerevisiae: characterization and modulation of activity Biochim Biophys Acta. 524:121-130.
28. Ossig, R , C. Dascher. H.-H. Trepte, H. D. Schmitt, and D. Gallwitz. 1991. The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport. Mol. Cell. Biol 11.2980-2993.
29. Preuss, D., J. Mulholland, A. Franzusoff, N Segev, and D. Botstein. 1992. Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy. Mol. Biol. Cell. 3:789-803.
30. Pryer, N., L. Wuestehube, and R. Schekman. 1992. Vesicle-mediated protein sorting. Annu. Rev. Biochem. 61:471-516.
31. Redding, K., C. Holcomb, and R. S. Fuller. 1991. Immunolocalization of Kex2 protease identifies a putative late Golgi compartment in the yeast Saccharomyces cerevisiae J. Cell Biol. 113:527-538
32. Rexach, M. R., and R. W. Schekman. 1991. Distinct biochemical requirements for budding, targeting and fusion of ER-derived transport vesicles. J. Cell Biol. 114:219-229.
33. Rothman, J E. 1994. Mechanisms of intracellular protein transport. Nature (Lond.). 372:55-63.
34. Sapperstein, S K., D. M. Walter, A. R. Grosvenor, J. E. Heuser, and M. G. Waters. 1995. p115 is a general vesicular transport factor related to the yeast endoplasmic reticulum to Golgi transport factor Uso1p. Proc. Natl Acad. Sci. USA 92:522-526
35. Sapperstein, S. K., V. V Lupashin, H. D. Schmitt, and M. G. Waters. 1996. Assembly of the ER to Golgi SNARE complex requires Uso1p J Cell Biol. In press.
36. Segev, N 1991. Mediation of the attachment of fusion step in vesicular transport by the GTP-bindmg Ypt1 protein. Science (Wash. DC). 252:1553-1556.
37. Seog, D., M. Kito, K. Igarashi, K. Yoda, and M. Yamasaki. 1994. Molecular characterization of the USO1 gene product which is essential for vesicular transport in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun 200.647-653.
38. Søgaard, M., K. Tani, R. R Ye. S Geromanos, P. Tempst, T. Kirchhausen, J E. Rothman, and T Söllner. 1994. A rab protein is required for the assembly of SNARE complexes in the docking of transport vesicles. Cell 78:937-948
39. Söllner, T., M K. Bennett, S. W. Whiteheart, R H. Scheller, and J. E Rothman. 1993. A protein assembly-disassembly pathway in vitro that may correspond to sequential steps of sinaptic vesicle docking, activation, and fusion. Cell. 75:409-418.
40. Sollner, T., S W. Whiteheart, M. Brunner, H. Erdjument-Bromage, S. Geromanos, P. Tempst, and J. E. Rothman. 1993 SNAP receptors implicated in vesicle targeting and fusion. Nature (Lond ). 362.318-323
41. Waters, M. G., D. O. Clary, and J. E. Rothman. 1992. A novel 115-kD peripheral membrane protein is required for intercisternal transport in the Golgi stack. J. Cell Biol. 118:1015-1026.
42. Whitters, E. A., T. P. McGee, and V. Bankaitis. 1994. Purification and characterization of a late Golgi compartment from Saccharomyces cerevisiae. J. Biol Chem. 269.28106-28117.
43. Wilson, D. W , C. A. Wilcox, G C. Flynn, E. Chen, W.-J. Kuang, W. J. Henzel, M. R. Block, A. Ullrich, and J. E. Rothman. 1989. A fusion protein required for vesicle-mediated transport in both mammalian cells and yeast. Nature (Lond ), 339.355-359.
44. Wilson, D. W., S W Whiteheart, M. Wiedmann, M. Brunner, and J E. Rothman. 1992. A multisubunit particle implicated in membrane fusion. J. Cell Biol. 117:531-538.
45. Wuestehube, L J., and R. Schekman. 1992. Reconstitution of transport from the endoplasmic reticulum to the Golgi complex using an ER-enriched membrane fraction from yeast. Methods Enzymol 219:124-136
46. Wuesthube, L. J., A. Eun, S. Hamamoto, P. Korn, R Ram, and R. Schekman. 1994. New mutants of Saccharomyces cerevisiae affected in the transport of proteins from the endoplasmic reticulum to the Golgi complex