Mutational effects on inclusion body formation in the periplasmic expression of the immunoglobulin VL domain REI

Folding and Design

Volumn 1, Issue 2, 1996, Pages 77-89

  Chan, Winnie ^a   Helms, Larry R ^a   Brooks, Ian ^a   Lee, Grace ^a   Ngola, Sarah ^a   McNulty, Dean ^a   Maleeff, Beverly ^a   Hensley, Preston ^a   Wetzel, Ronald ^a,b  

^a GLAXOSMITHKLINE   (United Kingdom)

^b NONE   (United States)

Author keywords

Disulfide bonds; Folding intermediate; Light chain; Periplasmic inclusion bodies; Temperature sensitive folding mutations; Thermodynamic stability

Indexed keywords

IMMUNOGLOBULIN LIGHT CHAIN; PEPTIDE FRAGMENT; RECOMBINANT DNA; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CELL INCLUSION; CHEMICAL STRUCTURE; CHEMISTRY; DIMERIZATION; DRUG STABILITY; ELECTRON MICROSCOPY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; METABOLISM; MOLECULAR GENETICS; MUTATION; NUCLEOTIDE SEQUENCE; PROTEIN CONFORMATION; PROTEIN FOLDING; SOLUBILITY; THERMODYNAMICS; ULTRASTRUCTURE;

AMINO ACID SEQUENCE; BASE SEQUENCE; DIMERIZATION; DNA, RECOMBINANT; DRUG STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; INCLUSION BODIES; MICROSCOPY, ELECTRON; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; MUTATION; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SOLUBILITY; THERMODYNAMICS;

EID: 0030334646     PISSN: 13590278     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1359-0278(96)00017-X     Document Type: Article

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