Recombinant human O6-alkylguanine-DNA alkyltransferase (AGT), Cys145-alkylated AGT and Cys145 → Met145 mutant AGT: Comparison by isoelectric focusing, CD and time-resolved fluorescence spectroscopy

Biochemical Journal

Volumn 324, Issue 1, 1997, Pages 321-328

  Federwisch, Matthias ^a,c   Hassiepen, Ulrich ^b   Bender, Kirsten ^a   Dewor, Manfred ^b   Rajewsky, Manfred F ^a   Wollmer, Axel ^b  

^a UNIVERSITY OF ESSEN MEDICAL SCHOOL   (Germany)

^b RWTH AACHEN UNIVERSITY   (Germany)

^c INSTITUTE OF MOLECULAR BIOLOGY   (Armenia)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; METHIONINE; METHYLATED DNA PROTEIN CYSTEINE METHYLTRANSFERASE; METHYLTRANSFERASE; PRIMER DNA; RECOMBINANT PROTEIN; TRYPTOPHAN; 6 O ALKYLGUANINE DNA ALKYLTRANSFERASE; GUANINE DERIVATIVE; RECOMBINANT ENZYME;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; HUMAN; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; POINT MUTATION; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; SPECTROFLUOROMETRY; TIME; ALKYLATION; ALPHA HELIX; AMINO ACID SUBSTITUTION; COMPARATIVE STUDY; FLUORESCENCE SPECTROSCOPY; ISOELECTRIC POINT; METHYLATION; PH; PRECIPITATION; PRIORITY JOURNAL; TEMPERATURE;

AMINO ACID SEQUENCE; CIRCULAR DICHROISM; CYSTEINE; DNA PRIMERS; HUMANS; ISOELECTRIC FOCUSING; KINETICS; METHIONINE; METHYLTRANSFERASES; MUTAGENESIS, SITE-DIRECTED; O(6)-METHYLGUANINE-DNA METHYLTRANSFERASE; POINT MUTATION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; TIME FACTORS; TRYPTOPHAN;

EID: 0030904275     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/bj3240321     Document Type: Review

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