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Volumn 277, Issue 5329, 1997, Pages 1094-1097

Functional role of high-affinity anandamide transport, as revealed by selective inhibition

Author keywords

[No Author keywords available]

Indexed keywords

ANANDAMIDE;

EID: 0030865871     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5329.1094     Document Type: Article
Times cited : (735)

References (41)
  • 1
    • 0027078685 scopus 로고
    • W. A. Devane et al., Science 258, 1946 (1992); R. Mechoulam, L. Hanuš, B. R. Martin, Biochem. Pharmacol. 48, 1537 (1994).
    • (1992) Science , vol.258 , pp. 1946
    • Devane, W.A.1
  • 4
    • 0028589390 scopus 로고
    • V. Di Marzo et al., Nature 372, 686 (1994).
    • (1994) Nature , vol.372 , pp. 686
    • Di Marzo, V.1
  • 6
    • 0029798721 scopus 로고    scopus 로고
    • H. Cadas, S. Gaillet, M. Beltramo, L. Venance, D. Piomelli, J. Neurosci. 16, 3934 (1996); T. Sugiura et al., Eur. J. Biochem. 240, 53 (1996); H. Cadas, E. di Tomaso, D. Piomelli, J. Neurosci., 17, 1226 (1997).
    • (1996) Eur. J. Biochem. , vol.240 , pp. 53
    • Sugiura, T.1
  • 7
    • 0031033841 scopus 로고    scopus 로고
    • H. Cadas, S. Gaillet, M. Beltramo, L. Venance, D. Piomelli, J. Neurosci. 16, 3934 (1996); T. Sugiura et al., Eur. J. Biochem. 240, 53 (1996); H. Cadas, E. di Tomaso, D. Piomelli, J. Neurosci., 17, 1226 (1997).
    • (1997) J. Neurosci. , vol.17 , pp. 1226
    • Cadas, H.1    Di Tomaso, E.2    Piomelli, D.3
  • 8
    • 0027180394 scopus 로고    scopus 로고
    • D. G. Deutsch and S. Chin, Biochem. Pharmacol. 46, 791 (1993); F. Desarnaud, H. Cadas, D. Piomelli, J. Biol. Chem. 270, 6030 (1995) ; N. Ueda, Y. Kurahashi, S. Yamamoto, T. Tokunaga, ibid., p. 23823.
    • (1993) Biochem. Pharmacol. , vol.46 , pp. 791
    • Deutsch, D.G.1    Chin, S.2
  • 9
    • 0028903996 scopus 로고
    • D. G. Deutsch and S. Chin, Biochem. Pharmacol. 46, 791 (1993); F. Desarnaud, H. Cadas, D. Piomelli, J. Biol. Chem. 270, 6030 (1995) ; N. Ueda, Y. Kurahashi, S. Yamamoto, T. Tokunaga, ibid., p. 23823.
    • (1995) J. Biol. Chem. , vol.270 , pp. 6030
    • Desarnaud, F.1    Cadas, H.2    Piomelli, D.3
  • 11
    • 0029904838 scopus 로고    scopus 로고
    • B. Cravatt et al., Nature 384, 83 (1996).
    • (1996) Nature , vol.384 , pp. 83
    • Cravatt, B.1
  • 13
    • 1842396907 scopus 로고
    • L. Z. Bito, Nature 256, 1234 (1975); J. E. Schaffer and H. F. Lodish, Cell 79, 427 (1994); I. N. Bojesen and E. Bojesen, Acta Physiol. Scand. 156, 501 (1996).
    • (1975) Nature , vol.256 , pp. 1234
    • Bito, L.Z.1
  • 14
    • 0028152492 scopus 로고
    • L. Z. Bito, Nature 256, 1234 (1975); J. E. Schaffer and H. F. Lodish, Cell 79, 427 (1994); I. N. Bojesen and E. Bojesen, Acta Physiol. Scand. 156, 501 (1996).
    • (1994) Cell , vol.79 , pp. 427
    • Schaffer, J.E.1    Lodish, H.F.2
  • 15
    • 0029983855 scopus 로고    scopus 로고
    • L. Z. Bito, Nature 256, 1234 (1975); J. E. Schaffer and H. F. Lodish, Cell 79, 427 (1994); I. N. Bojesen and E. Bojesen, Acta Physiol. Scand. 156, 501 (1996).
    • (1996) Acta Physiol. Scand. , vol.156 , pp. 501
    • Bojesen, I.N.1    Bojesen, E.2
  • 16
    • 0029078231 scopus 로고
    • N. Kanai et al., Science 268, 866 (1995).
    • (1995) Science , vol.268 , pp. 866
    • Kanai, N.1
  • 22
    • 0017228361 scopus 로고
    • L. Z. Bito, H. Davson, E. V. Salvador, J. Physiol. 256, 257 (1976); W. Stremmel and P. D. Berk, Proc. Natl. Acad. Sci. U.S.A. 83, 3086 (1986) ; S. Ruetz and P. Gros, Cell 77, 1071 (1994).
    • (1976) J. Physiol. , vol.256 , pp. 257
    • Bito, L.Z.1    Davson, H.2    Salvador, E.V.3
  • 23
    • 0022723903 scopus 로고
    • L. Z. Bito, H. Davson, E. V. Salvador, J. Physiol. 256, 257 (1976); W. Stremmel and P. D. Berk, Proc. Natl. Acad. Sci. U.S.A. 83, 3086 (1986) ; S. Ruetz and P. Gros, Cell 77, 1071 (1994).
    • (1986) Proc. Natl. Acad. Sci. U.S.A. , vol.83 , pp. 3086
    • Stremmel, W.1    Berk, P.D.2
  • 24
    • 0028307550 scopus 로고
    • L. Z. Bito, H. Davson, E. V. Salvador, J. Physiol. 256, 257 (1976); W. Stremmel and P. D. Berk, Proc. Natl. Acad. Sci. U.S.A. 83, 3086 (1986) ; S. Ruetz and P. Gros, Cell 77, 1071 (1994).
    • (1994) Cell , vol.77 , pp. 1071
    • Ruetz, S.1    Gros, P.2
  • 25
    • 1842351332 scopus 로고    scopus 로고
    • note
    • m values were 0.24 and 0.25 μM, respectively (n = 6).
  • 27
    • 1842270589 scopus 로고    scopus 로고
    • note
    • 2; 171 Ci/mmol, New England Nuclear). After rinsing with Krebs buffer containing BSA, we subjected the cells to lipid extraction and counted radioactivity in the extracts. On average, neurons contained 245 ± 65 dpm per well and astrocytes 302 ± 20 dpm per well; nonspecific accumulation in astrocytes at 0° to 4°C was 355 ± 28 dpm per well (n = 6).
  • 29
    • 1842272519 scopus 로고    scopus 로고
    • note
    • max values were 29 pmol/min per milligram of protein without AM404 and 26 pmol/min per milligram of protein with AM404, respectively (n = 6).
  • 30
    • 0025236913 scopus 로고
    • M. Herkenham et al., Proc. Natl. Acad. Sci. U.S.A. 87, 1932 (1990); L. A. Matsuda, T. I. Bonner, S. J. Lolait, J. Comp. Neurol. 327, 535 (1993).
    • (1990) Proc. Natl. Acad. Sci. U.S.A. , vol.87 , pp. 1932
    • Herkenham, M.1
  • 33
    • 0031036959 scopus 로고    scopus 로고
    • A. C. Howlett, J. M. Qualy, L. L. Khachatran, Mol. Pharmacol. 29, 307 (1986); M. Jung et al., J. Neurochem. 68, 402 (1997).
    • (1997) J. Neurochem. , vol.68 , pp. 402
    • Jung, M.1
  • 34
    • 1842387098 scopus 로고    scopus 로고
    • note
    • Cortical neurons were prepared in 12-well plates and used after 4 to 6 days in vitro. Incubations were carried out in the presence of forskolin (3 μM) and isobutyl methyl xanthine (1 mM). The cAMP concentrations were measured by radioimmunoassay with a commercial kit (Amersham, Arlington, IL) and following manufacturer's instructions.
  • 37
    • 1842352377 scopus 로고    scopus 로고
    • note
    • The amounts of cAMP in the presence of a concentration of WIN-55212-2 below threshold (1 nM, determined in preliminary experiments) were 96.7 ± 2.5% of forskolin alone and were not significantly affected by 10 μM AM404 (89.8 ± 2.6%), 10 μM AM403 (92.4 ± 2.3%), or 10 μM bromcresol green (92.9 ± 2.3%) (n = 3). In the presence of a concentration of glutamate below threshold (3 μM) (24), cAMP concentrations were 91.6 ± 2% of forskolin alone and were not significantly affected by AM404 (84.4 ± 4.9%), AM403 (89.5 ± 2.4%), or bromcresol green (84.4 ± 3%) (n = 3).
  • 38
  • 39
    • 0028291118 scopus 로고
    • E. Fride and R. Mechoulam, Eur. J. Pharmacol. 231, 313 (1993); P. B. Smith et al., J. Pharmacol. Exp. Ther. 270, 219 (1994).
    • (1994) J. Pharmacol. Exp. Ther. , vol.270 , pp. 219
    • Smith, P.B.1
  • 40
    • 0021215268 scopus 로고
    • The hot plate test (55.5°C) was carried out on male Swiss mice (25 to 30 g, Nossan, Italy) following standard procedures [F. Porreca, H. L. Mosberg, R. Hurst, V. J. Hruby, T. F. Burks, J. Pharmacol. Exp. Ther. 230, 341 (1994)]. Anandamide and AM404 were dissolved in 0.9% NaCl solution containing 20% dimethyl sulfoxide and injected intravenously at 20 mg/kg and 10 mg/kg, respectively. To determine whether cannabinoid receptors participate in the effect of anandamide, we administered anandamide (20 mg/kg intravenously) or anandamide plus SR141716-A (2 mg/kg, subcutaneously) to two groups of six mice each. In mice that received anandamide alone, latency to jump increased from 21.7 ± 1.5 s to 30.7 ± 0.8 s (P < 0.05, ANOVA) 20 min after injection. In contrast, in mice that received anandamide plus SR141716-A, the latency to jump was not affected (19.6 ± 3.1 s).
    • (1994) J. Pharmacol. Exp. Ther. , vol.230 , pp. 341
    • Porreca, F.1    Mosberg, H.L.2    Hurst, R.3    Hruby, V.J.4    Burks, T.F.5
  • 41
    • 1842307203 scopus 로고    scopus 로고
    • note
    • We thank E. di Tomaso and H. Cadas for help and E. Barker, L. Parsons, and P. Schweitzer for critical reading of the manuscript. Supported by the Neuroscience Research Foundation, which receives major support from Novartis.


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