CpG motifs in bacterial DNA cause inflammation in the lower respiratory tract

Journal of Clinical Investigation

Volumn 100, Issue 1, 1997, Pages 68-73

  Schwartz, David A ^a,b   Quinn, Timothy J ^a   Thorne, Peter S ^a   Sayeed, Sadath ^b   Yi, Ae Kyung ^b   Krieg, Arthur M ^a,b  

^a UNIVERSITY OF IOWA   (United States)

^b UNIVERSITY OF IOWA   (United States)

Author keywords

CpG dinucleotides; DNA; Infection; Lung inflammation; Pulmonary disease

Indexed keywords

BACTERIAL DNA;

ANIMAL EXPERIMENT; ARTICLE; BACTERIAL GENE; CYSTIC FIBROSIS; IMMUNOSTIMULATION; INFECTION RISK; LOWER RESPIRATORY TRACT INFECTION; MALE; MOUSE; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; SPUTUM ANALYSIS;

EID: 0030859292     PISSN: 00219738     EISSN: None     Source Type: Journal    
DOI: 10.1172/JCI119523     Document Type: Article

References (28)

1. Krieg, A.M., A.K. Yi, S. Matson, T.J. Waldschmidt, G.A. Bishop, R. Teasdale, G.A. Koretzky, and D.M. Klinman. 1995. CpG Motifs in bacterial DNA trigger direct B-cell activation. Nature (Lond.). 374:546-549.
2. Messina, J.P., G.S. Gilkeson, and D.S. Pisetsky. 1991. Stimulation of in vitro murine lymphocyte proliferation by bacterial DNA. J. Immunol. 147: 1759-1764.
3. Klinman, D.M., A.K. Yi, S.L. Beaucage, J. Conover, and A. Krieg. 1996. CpG Motifs present in bacterial DNA rapidly induce lymphocytes to secrete interleukin 6, interleukin 12, and interferon γ. Proc. Natl. Acad. Sci. USA. 93: 2879-2883.
4. Yi, A.-K., P. Hornbeck, D.E. Lafrenz, and A.M. Krieg. 1996. CpG DNA rescue of murine B lymphoma cells from anti-IgM induced growth arrest and programmed cell death is associated with increased expression of c-myc and bcl-XL. J. Immunol. 157:4918-4925.
5. Yi, A.-K., D.M. Klinman, T.L. Martin, S. Matson, and A.M. Krieg. 1996. Rapid immune activation by CpG motifs in bacterial DNA: systemic induction of IL-6 transcription through an antioxidant-sensitive pathway. J. Immunol. 157:5394-5402.
6. Cowdery, J.S., J.H. Chace, and A.M. Krieg. 1996. Bacterial DNA Induces NK cells to produce Interferon-gamma in vivo and increase the toxicity of lipopolysaccharides. J. Immunol. 156:4570-4575.
7. Ballas, Z.K., W.L. Rasmussen, and A.M. Krieg. 1996. Induction of natural killer activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA. J. Immunol. 157:1840-1845.
8. Tokunaga, T., H. Yamamoto, S. Shimada, H. Abe, T. Fukuda, Y. Fujisawa, Y. Furutani, O. Yano, T. Kataoka, T. Sudo, et al. 1984. Antitumor activity of deoxyribonucleic acid fraction from mycobacterium bovis GCG. I. Isolation, physicochemical characterization, and antitumor activity. J. Natl. Cancer Inst. 72:955-962.
9. Kuramoto, E., O. Yano, Y. Kimura, M. Baba, T. Makino, S. Yamamoto, T. Yamamoto, T. Kataoka, and T. Tokunaga. 1992. Oligonucleotide sequences required for natural killer cell activation. Jpn. J. Cancer Res. 83:1128-1131.
10. Chace, J.H., N.A. Hooker, K.L. Mildenstein, A.M. Krieg, and J.S. Cowdery. 1997. Bacterial DNA-induced NK cell IFN-γ production is dependent on macrophage secretion of IL-12. Clin. Immunol. Immunopathol. In press.
11. Bird, A.P. 1987. CpG islands as gene markers in the vertebrate nucleus. Trends Genet. 3:342-347.
12. Krieg, A.M. 1996. An innate immune defense mechanism based on the recognition of CpG motifs in microbial DNA. J. Lab. Clin. Med. 128:128-133.
13. Bennet, R.M., G.T. Gabor, and M.M. Merritt. 1985. DNA binding to human leukocytes. Evidence for a receptor-mediated association, internalization, and degradation of DNA. J. Clin. Invest. 76:2182-2190.
14. Zhao, Q., X. Song, T. Waldschmidt, E. Fisher, and A.M. Krieg. 1996. Oligonucleotide uptake in human hematopoietic cells is increased in leukemia and is related to cellular activation. Blood. 88:1788-1795.
15. Krieg, A.M. 1995. Uptake and localization of phosphodiester and chimeric oligodeoxynucleotides in normal and leukemic primary cells. In Delivery Strategies for Antisense Oligonucleotide Therapeutics. S. Akhtar, editor. CRC Press, Inc., Boca Raton, FL. 177-190.
16. Jagielo, P.J., P.S. Thorne, J.A. Kern, T.J. Quinn, and D.A. Schwartz. 1996. Role of endotoxin in grain dust-induced lung inflammation in mice. Am. J. Physiol. (Lung Cell. Mol. Physiol.) 270:L1052-L1059.
17. Goldberg, J.B., and D.E. Ohman. 1984. Cloning and Expression in Pseudomonas aeruginosa of a Gene Involved in the Production of Alginate. J. Bacteriol. 158:1115-1121.
18. Leeper-Woodford, S.K., P.D. Carey, K. Byrne, C. Blocher, J.H. Sugerman, and A.A. Fowler. 1991. TNF: α and β subunits appear in the circulation during onset of sepsis-induced lung injury. Am. Rev. Respir. Dis. 143:1076-1082.
19. Chomczynski, P., and N. Sacchi. 1987. Single-step method of RNA isolation. Anal. Biochem. 162:156-159.
20. Kedzierski, W. 1991. Removal of DNA template from RNA transcription mixtures. Biotechniques. 10:210-214.
21. + T cells from young and old mice. J. Immunol. 150:3602-3614.
22. Rosner, B. 1980. Fundamentals of Biostatistics (3rd ed.). PWS-Kent, Boston. 375 pp.
23. Chernick, W.S., and G.J. Barbero. 1959. The composition of pulmonary secretions from patients with and without cystic fibrosis. Pediatrics. 24:739-745.
24. Potter, J., L.W. Matthews, J. Lemm, and J.S. Spector. 1960. Studies on pulmonary secretions. Am. J. Dis. Child. 100:493-495.
25. Matthews, L.W., S. Spector, J. Lemm, and J.L. Potter. 1963. Composition of tracheobronchial secretions in cystic fibrosis of the pancreas and bronchiectasis. Am. Rev. Respir. Dis. 88:199-204.
26. Yi, A.-K., J.H. Chace, J.S. Cowdery, and A.M. Krieg. 1996. IFN-γ promotes IL-6 and IgM secretion in response to CpG motifs in bacterial DNA and oligodeoxynucleotides. J Immunol. 156:558-564.
27. Sato, Y., M. Roman, H. Tighe, D. Lee, M. Corr, M.-D. Nguyen, G.J. Silverman, M. Lotz, D.A. Carson, and E. Raz. 1996. Immunostimulatory DNA sequences necessary for effective intradermal gene immunization. Science (Wash. DC). 273:352-354.
28. Rozenberg-Arska, M., J.A.G. van Strijp, W.P.M. Hoekstra, and J. Verhoef. 1984. Effect of human polymorphonuclear and mononuclear leukocytes on chromosomal and plasmid DNA of Escherichia coli. Role of acid DNase. J. Clin. Invest. 73:1254-1262.