KAR5 encodes a novel pheromone-inducible protein required for homotypic nuclear fusion

Journal of Cell Biology

Volumn 139, Issue 5, 1997, Pages 1063-1076

  Beh, Christopher T ^a,b   Brizzio, Valeria ^a   Rose, Mark D ^a  

^a PRINCETON UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PHEROMONE;

ARTICLE; CELL NUCLEUS; DELETION MUTANT; ENDOPLASMIC RETICULUM; FUNGAL GENETICS; HAPLOIDY; MEMBRANE FUSION; NONHUMAN; PRIORITY JOURNAL; PROTEIN INDUCTION; SCHIZOSACCHAROMYCES POMBE; SEQUENCE HOMOLOGY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CELL COMPARTMENTATION; CELL POLARITY; CLONING, MOLECULAR; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; GENES, FUNGAL; MEMBRANE FUSION; MEMBRANE PROTEINS; MITOTIC SPINDLE APPARATUS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; NUCLEAR ENVELOPE; NUCLEAR PROTEINS; PEPTIDES; PHEROMONES; PROTEIN STRUCTURE, SECONDARY; RESTRICTION MAPPING; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE ANALYSIS, DNA;

SCHIZOSACCHAROMYCES POMBE;

EID: 0030831596     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.139.5.1063     Document Type: Article

References (72)

1. Acharya, U., R. Jacobs, J.-M. Peters, N. Watson, M.G. Farquhar, and V. Malhotra. 1995. The formation of Golgi stacks from vesiculated Golgi membranes requires two distinct fusion events. Cell. 82:895-904.
2. Aris, J.P., and G. Blobel. 1991. Isolation of yeast nuclei. Methods Enzymol. 194: 735-749.
3. Beh, C.T. 1996. Functions of the yeast endoplasmic reticulum. Ph.D. Thesis, Princeton University, Princeton, NJ. 178pp.
4. Berlin, V., C.A. Styles, and G.A. Fink. 1990. BIK1, a protein required for microtubule function during mating and mitosis in Saccharomyces cerevisiae. colocalizes with tubulin. J. Cell Biol. 111:2573-2586.
5. Berlin, V., J.A. Brill, J. Trueheart, J.D. Boeke, and G.R. Fink. 1991. Genetic screens and selections for cell and nuclear fusion mutants. Methods Enzymol. 194:774-792.
6. Boeke, J.D., J. Trueheart, G. Natsoulis, and G.R. Fink. 1987. 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol. 154: 164-175.
7. Bradford, M.M. 1976. A dye binding assay for protein. Anal. Biochem. 72:248-254.
8. Brodsky, J.L., and R. Schekman. 1993. A Sec63p-BiP complex from yeast is required for protein translocation in a reconstituted proteoliposome. J. Cell Biol. 123:1355-1363.
9. Byers, B. 1981. Cytology of the yeast life cycle. In The Molecular Biology of the Yeast Saccharomyces: Life Cycle and Inheritance. J.N. Strathern, E.W. Jones, and J.R. Broach, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 59-96.
10. Byers, B., and L. Goetsch. 1974. Duplication of spindle plaques and integration of the yeast cell cycle. Cold Spring Harbor Symp. Quant. Biol. 38:123-131.
11. Byers, B., and L. Goetsch. 1975. Behavior of spindles and spindle plaques in the cell cycle and conjugation in Saccharomyces cerevisiae. J. Bacteriol. 124:511-523.
12. Conde, J., and G.R. Fink. 1976. A mutant of Saccharomyces cerevisiae defective for nuclear fusion. Proc. Natl. Acad. Sci. USA. 73:3651-3655.
13. Dabora, S.L., and M.P. Sheetz. 1988. The microtubule-dependent formation of a tubulovesicular network with characteristics of the ER from cultured cell extracts. Cell. 54:27-35.
14. Delgado, M.A., and J. Conde. 1984. Benomyl prevents nuclear fusion in Saccharomyces cerevisiae. Mol. Gen. Genet. 193:188-189.
15. Feldheim, D., and R. Schekman. 1994. Sec72p contributes to the selective recognition of signal peptides by the secretory polypeptide translocation complex. J. Cell Biol. 126:935-943.
16. Feldheim, D., K. Yoshimura, A. Admon, and R. Schekman. 1993. Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum. Mol. Biol. Cell. 4:931-939.
17. Green, N., H. Fang, and P. Walter. 1992. Mutants in three novel complementation groups inhibit membrane protein insertion into and soluble protein translocation across the endoplasmic reticulum membrane of Saccharomyces cerevisiae. J. Cell Biol. 116:597-604.
18. Henikoff, S. 1987. Unidirectional digestion with exonuclease III in DNA sequence analysis Methods Enzymol. 155:156-165.
19. Hoffman, C.S., and F. Winston. 1987. A ten minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of E. coli. Gene. 57:267-272.
20. Hofmann, K., and W. Stoffel. 1993. TMBASE - a database of membrane spanning protein segments. Biol. Chem. 374:166.
21. Hopp, T.P. 1989. Use of hydrophilicity plotting procedures to identify protein antigenic segments and other interaction sites. Methods Enzymol. 178:571-585.
22. Huffaker, T.C., M.A. Hoyt, and D. Botstein. 1987. Genetic analysis of the yeast cytoskeleton. Annu. Rev. Genet. 21:259-284.
23. Huffaker, T.C., J.H. Thomas, and D. Botstein. 1988. Diverse effects of β-tubulin mutations on microtubule formation and function. J. Cell Biol. 106:1997-2010.
24. Kurihara, L.J., and P. Silver. 1993. Suppression of a sec63 mutation identifies a novel component of the yeast endoplasmic reticulum translocation apparatus. Mol. Biol. Cell. 4:919-930.
25. Kurihara, L.J., C.T. Beh, M. Latterich, R. Schekman, and M. Rose. 1994. Nuclear congression and membrane fusion: two distinct events in the yeast karyogamy pathway. J. Cell Biol. 126:911-923.
26. Kurihara, L.J., B. Stewart, A. Gammie, and M.D. Rose 1996. Kar4p, a karyogamy-specific component of the yeast pheromone response pathway. Mol. Cell. Biol. 16:3990-4002.
27. Kyte, J., and R.F. Doolittle. 1982. A simple method for displaying the hydropathic character of a protein. J. Mol. Biol. 157:105-132.
28. Laemmli, E.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680-685.
29. Latterich, M., and R. Schekman. 1994. The karyogamy gene KAR2 and novel proteins are required for ER-membrane fusion. Cell. 78:87-98.
30. Lupas, A. 1996. Prediction and analysis of coiled-coil structures. Method Enzymol. 266:513-525.
31. Marsh, L., and M.D. Rose. 1997. The pathway of cell and nuclear fusion during mating in Saccharomyces cerevisiae. In The Molecular and Cellular Biology of the Yeast Saccharomyces, Vol. 3, Cell Cycle and Cell Biology. J.R. Pringle, J.R. Broach, and E.W. Jones, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor. NY. 827-888.
32. McCaffrey, G., F.J. Clay, K. Kelsay, and G.F. Sprague. 1987. Identification and regulation of a gene required for cell fusion during mating of the yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 7:2680-2690.
33. Melikyan, G.B., S.C.A. Brener, D.C. Ok, and F.S. Cohen. 1997. Inner but not outer membrane leaflets control the transition from gylosylphosphatidylinositol-anchores influenza hemagglutinin-induced hemifusion to full fusion. J. Cell Biol. 136:995-1005.
34. Meluh, P.B., and M.D. Rose. 1990. KAR3, a kinesin-related gene required for yeast nuclear fusion. Cell. 60:1029-1041.
35. Ng, D.T., and P. Walter. 1996. ER membrane protein complex required for nuclear fusion. J. Cell Biol. 132:499-509.
36. Nishikawa, S., and T. Endo. 1997. The yeast JEM1p is a DnaJ-like protein of the endoplasmic reticulum membrane required for nuclear fusion. J. Biol. Chem. 272:12889-12892.
37. Normington, K., K. Kohno, Y. Kozutsumi, M.J. Gething, and J. Sambrook. 1989. S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP. Cell. 57:1223-1236.
38. Novick, P., C. Field, and R. Schekman. 1980. Identification of 23 complementation groups required for post-translational events in the yeast secretory pathway. Cell. 21:205-215.
39. Ohashi, A., J. Gibson, I. Gregor, and G. Schatz. 1982. Import of proteins into mitochondria. J. Biol. Chem. 257:13042-13047.
40. Osborne, M.A., G. Schlenstedt, T. Jinks, and P.A. Silver. 1994. Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J. Cell Biol. 125:853-866.
41. Page, B.D., and M. Snyder. 1992. CIK1: a developmentally regulated spindle pole body-associated protein important for microtubule functions in Saccharomyces cerevisiae. Genes Dev. 6:1414-1429.
42. Page, B.D., L.L. Satterwhite, M.D. Rose, and M. Snyder. 1994. Localization of the KAR3 kinesin heavy chain-like protein requires the CIK1 interacting protein. J. Cell Biol. 124:507-519.
43. Polaina, J., and J. Conde. 1982. Genes involved in the control of nuclear fusion during the sexual cycle of Saccharomyces cerevisiae. Mol. Gen. Genet. 186: 253-258.
44. Rabouille, C., T.P. Levine, J.-M. Peters, and G. Warren. 1995. An NSF-like ATPase, p97, and NSF mediate cisternal regrowth from mitotic Golgi fragments. Cell. 82:905-914.
45. Riles, L., J.E. Dutchik, A. Baktha, B.K. McCauley, E.C. Thayer, M.P. Leckie, V.V. Braden, J.E. Depke, and M.V. Olson. 1993. Physical maps of the six smallest chromosomes of Saccharomyces cerevisiae at a resolution of 2.6 kilobase pairs. Genetics. 134:81-150.
46. Roberts, C.J., C.K. Raymond, C.T. Yamashiro, and T.H. Stevens. 1991. Methods for studying the yeast vacuole. Methods Enzymol. 194:644-661.
47. Rose, M.D. 1991. Nuclear fusion in yeast. Annu. Rev. Microbiol. 45:539-567.
48. Rose, M.D. 1996. Nuclear fusion in the yeast Saccharomyces cerevisiae. Annu. Rev. Cell Dev. Biol. 12:663-695.
49. Rose, M.D., and G.R. Fink. 1987. KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeast. Cell. 48:1047-1060.
50. Rose, M.D., P. Novick, J.H. Thomas, D. Botstein, and G.R. Fink. 1987. A Saccharomyces cerevisiae genomic plasmid bank based on a centromere-containing shuttle vector. Gene. 60:237-243.
51. Rose, M.D., L.M. Misra, and J.P. Vogel. 1989. KAR2, a karyogamy gene, is the yeast homologue of the mammalian BiP/GRP78 gene. Cell. 57:1211-1221.
52. Rose, M.D., F. Winston, and P. Hieter. 1990. Methods of Yeast Genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 198 pp.
53. Rost, B., R. Casadio, P. Fariselli, and C. Sander. 1995. Prediction of helical transmembrane segments at 95% accuracy. Prot. Sci. 4:521-533.
54. Rothman, J.E. 1994. Mechanisms of intracellular protein transport. Nature. 372: 55-63.
55. Rothman, J.E., and G. Warren. 1994. Implications of the SNARE hypothesis for intracellular membrane topology and dynamics. Curr. Biol. 4:220-233.
56. Rout, M.P., and J.V. Kilmartin. 1990. Components of the yeast spindle and spindle pole body. J. Cell Biol. 111:1913-1917.
57. Sadler, I., A. Chiang, T. Kurihara, J. Rothblatt, J. Way, and P. Silver. 1989. A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein. J. Cell Biol. 109:2665-2675.
58. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
59. Scidmore, M.A., H. Okomura, and M.D. Rose. 1993. Genetic interaction between KAR2 and SEC63, eukaryotic homologues of HSP70 and DNAJ in the endoplasmic reticulum. Mol. Biol. Cell. 4:1145-1159.
60. Sikorski, R., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19-27.
61. Smith, D.B., and K.S. Johnson. 1988. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 67:31-40.
62. Spang, A., I. Courtney, K. Grein, M. Matzner, and E. Schiebel. 1995. The Cdc31p-binding protein Kar1p is a component of the half bridge of the yeast spindle pole body. J. Cell Biol. 128:863-877.
63. Sprague, G.F., and J. Thorner. 1992. Pheromone response and signal transduction during the mating process of Saccharomyces cerevisiae. In The Molecular Biology of the Yeast Saccharomyces, 2nd ed. J.R. Broach, J.R. Pringle, and E.W. Jones, editors. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 657-744.
64. Stirling, C.J., J. Rothblatt, M. Hosobuchi, R. Deshaies, and R. Schekman. 1992. Protein translocation mutants defective in the insertion of integral membrane proteins into the endoplasmic reticulum. Mol. Biol. Cell. 3:129-142.
65. Toyn, J., A.R. Hibbs, P. Sanz, J. Crowe, and D.I. Meyer. 1988. In vivo and in vitro analysis of ptl1, a yeast ts mutant with a membrane-associated defect in protein translocation. EMBO (Eur. Mol. Biol. Organ.) J. 7:4347-4353.
66. Trueheart, J., J.D. Boeke, and G.R. Fink. 1987. Two genes required for cell fusion during conjugation: evidence for a pheromone-induced surface protein. Mol. Cell. Biol. 7:2316-2328.
67. Vallen, E.A., T.Y. Scherson, T. Roberts, K. van Zee, and M.D. Rose. 1992. Asymmetric mitotic segregation of the yeast spindle pole body. Cell. 69:505-515.
68. Vogel, J.P., L. Misra, and M.D. Rose. 1990. Loss of BiP/GRP78 function blocks translocation of secretory proteins in yeast. J. Cell Biol. 110:1885-1895.
69. von Heijne, G. 1986. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res. 14:4683-4690.
70. White, J.M. 1990. Viral and cellular membrane fusion proteins. Annu. Rev. Physiol. 52:675-697.
71. Wiese, C. and K.L. Wilson. 1993. Nuclear membrane dynamics. Curr. Opin. Cell Biol. 5:387-394.
72. Winey, M., and B. Byers. 1993. Assembly and functions of the spindle pole body in budding yeast. Trends Genet. 9:300-304.