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0029020945
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Involvement of the cell-cylce inhibitor Cip 1.WAF1 and the E1A-associated p300 chain protein in terminal differentiation
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Inhibition of retinoblastoma protein phosphorylation by myogenesis-induced changes in the subunit composition of the cyclin-dependent kinase 4 complex
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Wang J, Walsh K. Inhibition of retinoblastoma protein phosphorylation by myogenesis-induced changes in the subunit composition of the cyclin-dependent kinase 4 complex. Cell Growth Differ. 7:1996;1471-1478.
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Walsh, K.2
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Myogenin expression, cell cycle withdrawal and phenotypic differentiation are temporally separable events that precede cell fusion upon myogenesis
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of special interest. This study investigated the temporal relationships among the events that govern the transition from proliferative myoblasts to the terminally differentiated multinucleated myocytes. Despite the asynchrony in the commitment to differentiation, skeletal myogenesis was found to be a highly ordered process involving at least four temporally separable events: first, entry of myoblasts into the differentiation pathway, as indicated by the induction of myogenin; second, irreversible cell cycle withdrawal, as indicated by the expression of p21; third, phenotypic differentiation, as indicated by the induction of myosin heavy chain (MHC); and fourth, cell fusion. This study provided a temporal framework that permitted further dissection of the regulatory networks that couple differentiation, proliferation, and apoptosis during skeletal myogenesis.
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Andrés V, Walsh K. Myogenin expression, cell cycle withdrawal and phenotypic differentiation are temporally separable events that precede cell fusion upon myogenesis. of special interest J Cell Biol. 132:1996;657-666 This study investigated the temporal relationships among the events that govern the transition from proliferative myoblasts to the terminally differentiated multinucleated myocytes. Despite the asynchrony in the commitment to differentiation, skeletal myogenesis was found to be a highly ordered process involving at least four temporally separable events: first, entry of myoblasts into the differentiation pathway, as indicated by the induction of myogenin; second, irreversible cell cycle withdrawal, as indicated by the expression of p21; third, phenotypic differentiation, as indicated by the induction of myosin heavy chain (MHC); and fourth, cell fusion. This study provided a temporal framework that permitted further dissection of the regulatory networks that couple differentiation, proliferation, and apoptosis during skeletal myogenesis.
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Andrés, V.1
Walsh, K.2
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10
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0028238952
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Cyclins and cyclin-dependent kinases are differentially regulated during terminal differentiation of C2C12 muscle cells
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Jahn L, Sadoshima J-I, Izumo S. Cyclins and cyclin-dependent kinases are differentially regulated during terminal differentiation of C2C12 muscle cells. Exp Cell Res. 212:1994;297-307.
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Jahn, L.1
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Inhibition of myogenic differentiation in proliferating myoblasts by cyclin D1-dependent kinase
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Skapek SX, Rhee J, Spicer DB, Lassar AB. Inhibition of myogenic differentiation in proliferating myoblasts by cyclin D1-dependent kinase. Science. 267:1995;1022-1024.
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Skapek, S.X.1
Rhee, J.2
Spicer, D.B.3
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CIP1 Cdk inhibitor family, is a candidate tumor suppressor gene
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CIP1 Cdk inhibitor family, is a candidate tumor suppressor gene. Genes Dev. 9:1995;650-662.
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KIP2, a cylin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 9:1995;639-649.
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Lee, M.-H.1
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INK4c and its predominant association with CDK4 and CDK6 during myogenic differentiation
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INK4c and its predominant association with CDK4 and CDK6 during myogenic differentiation. Mol Biol Cell. 7:1996;1587-1599.
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Expression of the positive regulator of cell cycle progression, cyclin D3, is induced during differentiation of myoblasts into quiescent myotubes
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Kiess M, Gill RM, Hamel PA. Expression of the positive regulator of cell cycle progression, cyclin D3, is induced during differentiation of myoblasts into quiescent myotubes. Oncogene. 10:1995;159-166.
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Kiess, M.1
Gill, R.M.2
Hamel, P.A.3
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P35 is a neural-specific regulatory subunit of cyclin-dependent kinase 5
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Tsai, L.-H.1
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Lew J, Huang Q-Q, Qi Z, Winkfein RJ, Aebersold R, Hunt T, Wang JH. A brain-specific activator of cyclin-dependent kinase 5. Nature. 371:1994;423-426.
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Ectopic expression of cyclin D1 prevents activation of gene transcription by myogenic basic helix-loop-helix regulators
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Inhibition of myogenesis by multiple cyclin/cdk complexes: Coordinate regulation of myogenesis and cell cycle activity at the level of E2F
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of special interest. The authors of this report analyzed the mechanisms that establish the antagonistic relationship between cell cycle and myogenic differentiation. Overexpression of each D-type cyclin was found to be sufficient to inhibit myogenic transcription. Furthermore, overexpression of cyclins A or E could also inhibit myogenic transcription but only when they were co-expressed with their catalytic partner cdk2. Inhibition of myogenic transcription was reversed by the co-expression of p21 or by hyperactive mutants of Rb. The status of myogenic transcription was found to correlate inversely with E2F transcriptional activity. It was proposed that inhibition of myogenic transcription is a general feature of cyclin/cdk activity and that differentiation is coordinated with cell cycle activity at the level of E2F.
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Guo K, Walsh K. Inhibition of myogenesis by multiple cyclin/cdk complexes: coordinate regulation of myogenesis and cell cycle activity at the level of E2F. of special interest J Biol Chem. 272:1997;791-797 The authors of this report analyzed the mechanisms that establish the antagonistic relationship between cell cycle and myogenic differentiation. Overexpression of each D-type cyclin was found to be sufficient to inhibit myogenic transcription. Furthermore, overexpression of cyclins A or E could also inhibit myogenic transcription but only when they were co-expressed with their catalytic partner cdk2. Inhibition of myogenic transcription was reversed by the co-expression of p21 or by hyperactive mutants of Rb. The status of myogenic transcription was found to correlate inversely with E2F transcriptional activity. It was proposed that inhibition of myogenic transcription is a general feature of cyclin/cdk activity and that differentiation is coordinated with cell cycle activity at the level of E2F.
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Guo, K.1
Walsh, K.2
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Cyclin-mediated inhibition of muscle gene expression via a mechanism that is independent of pRB hyperphosphorylation
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Skapek SX, Rhee J, Kim PS, Novitch BG, Lassar AB. Cyclin-mediated inhibition of muscle gene expression via a mechanism that is independent of pRB hyperphosphorylation. Mol Cell Biol. 16:1996;7043-7053.
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Kim, P.S.3
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Resistance to apoptosis conferred by Cdk inhibitors during myocyte differentiation
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of outstanding interest. An analysis of the mechanisms that regulate apoptosis during myogenic differentiation. It is reported that differentiating myocytes either die or differentiate and that mature myotubes are resistant to apoptosis. It was found that apoptosis and the cell cycle are coordinately regulated. Analyses revealed that p21 induction coincides with the acquisition of the apoptosis-resistant phenotype. Furthermore, the forced expression of p21 protects differentiating myocytes from apoptosis.
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Wang J, Walsh K. Resistance to apoptosis conferred by Cdk inhibitors during myocyte differentiation. of outstanding interest Science. 273:1996;359-361 An analysis of the mechanisms that regulate apoptosis during myogenic differentiation. It is reported that differentiating myocytes either die or differentiate and that mature myotubes are resistant to apoptosis. It was found that apoptosis and the cell cycle are coordinately regulated. Analyses revealed that p21 induction coincides with the acquisition of the apoptosis-resistant phenotype. Furthermore, the forced expression of p21 protects differentiating myocytes from apoptosis.
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Wang, J.1
Walsh, K.2
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25
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0031050236
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Rb functions to inhibit apoptosis during myocyte differentiation
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of outstanding interest. This study demonstrates that pRb-deficient myocytes undergo a high frequency of apoptosis during differentiation. Overexpression of p21 or p16 does not inhibit apoptosis in these cells but the re-expression of pRb confers apoptosis-resistance. These data demonstrate that pRb functions downstream from the cdk inibitors to coordinate cell cycle withdrawal and programmed cell death during myogenesis.
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Wang J, Guo K, Walsh K. Rb functions to inhibit apoptosis during myocyte differentiation. of outstanding interest Cancer Res. 57:1997;351-354 This study demonstrates that pRb-deficient myocytes undergo a high frequency of apoptosis during differentiation. Overexpression of p21 or p16 does not inhibit apoptosis in these cells but the re-expression of pRb confers apoptosis-resistance. These data demonstrate that pRb functions downstream from the cdk inibitors to coordinate cell cycle withdrawal and programmed cell death during myogenesis.
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Wang, J.1
Guo, K.2
Walsh, K.3
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MyoD induces retinoblastoma gene expression during myogenic differentiation
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0029960519
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2 phases of the cell cycle
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-/- myocytes are blocked from mitosis. These cells were also defective in myosin heavy chain (MHC) expression. In contrast, myocytes lacking p107 or p130 appeared normal with respect to differentiation and irreversible cell cycle withdrawal.
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-/- myocytes are blocked from mitosis. These cells were also defective in myosin heavy chain (MHC) expression. In contrast, myocytes lacking p107 or p130 appeared normal with respect to differentiation and irreversible cell cycle withdrawal.
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Novitch, B.G.1
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Schneider JW, Gu W, Zhu L, Mahdavi V, Nadal-Ginard B. Reversal of terminal differentiation mediated by p107 in Rb-/- muscle cells. Science. 264:1997;1467-1471.
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Gu, W.2
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Nadal-Ginard, B.5
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0029819994
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PRb controls proliferation, differentiation, and death of skeletal muscle cells and other lineages during embryogenesis
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of special interest. of outstanding interest. The authors of this study analyzed skeletal muscle differentiation in transgenic mouse embryos that express low levels of pRb. Substantial cell death in muscle masses was detected prior to the onset of terminal differentiation. Surviving myocytes also appeared to be defective in cell cycle exit and the expression of late muscle-specific genes. Remarkably similar conclusions are derived from this study and from studies in myogenic cell lines [21,25,28]. Collectively, these reports suggest that muscle cell fate is dependent upon the status of the pRb complex.
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of special interest Zacksenhaus E, Jiang Z, Chung D, Marth JD, Phillips RA, Gallie BL. pRb controls proliferation, differentiation, and death of skeletal muscle cells and other lineages during embryogenesis. of outstanding interest Genes Dev. 10:1996;3051-3064 The authors of this study analyzed skeletal muscle differentiation in transgenic mouse embryos that express low levels of pRb. Substantial cell death in muscle masses was detected prior to the onset of terminal differentiation. Surviving myocytes also appeared to be defective in cell cycle exit and the expression of late muscle-specific genes. Remarkably similar conclusions are derived from this study and from studies in myogenic cell lines [21,25,28]. Collectively, these reports suggest that muscle cell fate is dependent upon the status of the pRb complex.
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Genes Dev
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Zacksenhaus, E.1
Jiang, Z.2
Chung, D.3
Marth, J.D.4
Phillips, R.A.5
Gallie, B.L.6
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Multiple changes in E2F function and regulation occur upon muscle differentiation
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Shin EK, Paulding C, Schaffhausen B, Yee AS. Multiple changes in E2F function and regulation occur upon muscle differentiation. Mol Cell Biol. 15:1995;2252-2262.
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Shin, E.K.1
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Characterization of transcription factor E2F complexes during muscle and neuronal differentiation
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Corbeil HB, Whyte P, Branton PE. Characterization of transcription factor E2F complexes during muscle and neuronal differentiation. Oncogene. 11:1995;909-920.
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Expression and activity of the retinoblastoma protein (pRB)- family proteins, p107 and p130, during L6 myoblast differentiation
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Kiess M, Gill RM, Hamel PA. Expression and activity of the retinoblastoma protein (pRB)- family proteins, p107 and p130, during L6 myoblast differentiation. Cell Growth Differ. 6:1995;1287-1298.
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Kiess, M.1
Gill, R.M.2
Hamel, P.A.3
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Deregulated transcription factor E2F-1 expression leads to S-phase entry and p53-mediated apoptosis
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Field, S.J.1
Tsai, F.-Y.2
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The role of cyclin-dependent kinase 5 and a novel regulatory subunit in regulating muscle differentiation and patterning
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of outstanding interest. It is shown that cdk5 activity is required for normal somitic muscle development. Blocking cdk5 with a dominant-negative mutant specifically disrupts somitic muscle patterning and suppressed MyoD, MRF4 and muscle actin expression.
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Philpott A, Porro EB, Kirschner MW, Tsai L-H. The role of cyclin-dependent kinase 5 and a novel regulatory subunit in regulating muscle differentiation and patterning. of outstanding interest Genes Dev. 11:1997;1409-1421 It is shown that cdk5 activity is required for normal somitic muscle development. Blocking cdk5 with a dominant-negative mutant specifically disrupts somitic muscle patterning and suppressed MyoD, MRF4 and muscle actin expression.
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Tsai, L.-H.4
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IGF-II is more active than IGF-I in stimulating L6A1 myogenesis: Greater mitogenic actions of IGF-I delay differentiation
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Ewton DZ, Roof SL, Magri KA, McWade FJ, Florini JR. IGF-II is more active than IGF-I in stimulating L6A1 myogenesis: greater mitogenic actions of IGF-I delay differentiation. J Cell Physiol. 161:1994;277-284.
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