The use of primary hepatocyte cultures for the evaluation of chemoprotective agents

Cell Biology and Toxicology

Volumn 13, Issue 4-5, 1997, Pages 323-329

  Morel, F ^a   Langouet S ^a   Maheo K ^a   Guillouzo, A ^a  

^a UNIVERSITÉ DE RENNES 1   (France)

Author keywords

Chemoprotection; Cytochrome P450; Glutathione S transferase; Hepatocytes; Human; In vitro preparation; Rat

Indexed keywords

AFLATOXIN B1; CYTOCHROME P450; GLUTATHIONE TRANSFERASE; OLTIPRAZ; SULFORAPHANE; UNCLASSIFIED DRUG;

ANIMAL CELL; CHEMOPROPHYLAXIS; CONFERENCE PAPER; CONTROLLED STUDY; DRUG SCREENING; ENZYME ACTIVITY; ENZYME INDUCTION; HUMAN; HUMAN CELL; IN VITRO STUDY; LIVER CELL CULTURE; NONHUMAN; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SPECIES DIFFERENCE;

ANIMALS; ANTICARCINOGENIC AGENTS; CARCINOGENICITY TESTS; CELLS, CULTURED; HUMANS; LIVER; PYRAZINES; RATS; THIOCYANATES; TOXICITY TESTS;

ANIMALIA; BRASSICA OLERACEA VAR. BOTRYTIS;

EID: 0030824718     PISSN: 07422091     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007491525955     Document Type: Conference Paper

References (28)

1. Blaauboer BJ, Boobis AE, Castell JV et al. The practical applicability of hepatocyte cultures in routine testing. ATLA. 1994;22:231-41.
2. Buetler TM, Gallagher EP, Changhong W, Stahl DL, Hayes JD, Eaton DL. Induction of phase I and phase II drug-metabolizing enzyme mRNA, protein and activity by BHA, ethoxyquin and oltipraz. Toxicol Appl Pharmacol. 1995;135:45-57.
3. Conoway CC, Jiao D, Chung F. Inhibition of rat liver cytochrome P-450 isoenzyme by isothiocyanate and their conjugates: a structure-activity relationship study. Carcinogenesis. 1996;17:2423-7.
4. Guguen-Guillouzo C, Clement B, Baffet G et al. Maintenance and reversibility of active albumin secretion by adult rat hepatocytes co-cultured with another liver epithelial cell type. Exp Cell Res. 1983;143:47-54.
5. Guillouzo A. Hépatotoxicité in vitro. In: Adolphe M, Guillouzo A, Marano F, eds. Toxicologie cellulaire in vitro. Paris: Les Editions INSERM; 1995a:69-120.
6. Guillouzo A. Acquisition and use of human in vitro liver preparations. Cell Biol Toxicol. 1995b;11:141-5.
7. Guillouzo A, Chesné C. Xenobiotic metabolism in epithelial cell cultures. In: Shaw AJ, ed. Cell culture models of epithelial tissues: A practical approach. Oxford: Oxford University Press; 1996:67-85.
8. Guillouzo A, Morel F, Ratanasavanh D, Chesné C, Guguen-Guillouzo C. Long-term culture of functional hepatocytes. Toxicol In Vitro. 1990;74:415-27.
9. Guyomard C, Rialland L, Fremond B, Chesné C, Guillouzo A. Influence of alginate gel entrapment and cryopreservation on survival and xenobiotic metabolism capacity of rat hepatocytes. Toxicol Appl Pharmacol. 1996;141:349-56.
10. 1-8,9-oxide. Biochem J. 1991;279:385-98.
11. Hayes JD, Pulford DJ. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol. 1995;30:445-600.
12. Hecht SS. Chemoprevention by isothiocyanates. J Cell Biochem. 1995;22:195-209.
13. Kensler TW, Helzlsouer KJ. Oltipraz: clinical opportunities for cancer chemoprevention. J Cell Biochem. 1995;22:101-7.
14. Kensler TW, Egner P, Dolan P, Groopman J, Roebuck B. Mechanism of chemoprotection against aflatoxin tumorigenicity in rats fed 5-(2-pyrazinyl)-4-methyl-1,2-dithiole-3-thione (oltipraz) and related 1,2-dithiole-3-thiones and 1,2-dithiole-3-ones. Cancer Res. 1987;47:4271-7.
15. 1 metabolism in human hepatocytes in primary culture. Cancer Res. 1995;55:5574-9.
16. Langouët S, Morel F, Meyer DJ et al. A comparison of the effect of inducers on the expression of glutathione S-transferases in the liver of the intact rat and in hepatocytes in primary culture. Hepatology. 1996;43:881-7.
17. Langouët S, Mahéo K, Berthou F et al. Effects of administration of the chemoprotective agent oltipraz on CYP1A and CYP2B in rat liver and rat hepatocytes in culture. 1997. Carcinogenesis. [in press].
18. Liu YH, Taylor J, Linko P, Lucier GW, Thompson CL. Glutathione S-transferase μ in human lymphocytes and liver: role in modulating formation of carcinogen-derived adducts. Carcinogenesis. 1991;12:2269-75.
19. Morel F, Beaune P, Ratanasavanh D et al. Expression of cytochrome P-450 enzymes in cultured human hepatocytes. Eur J Biochem. 1990;191:437-44.
20. Morel F, Fardel O, Meyer DJ et al. Preferential increase of glutathione S-transferase class alpha transcripts in cultured human hepatocytes by phenobarbital, 3-methylcholanthrene and dithiolethiones. Cancer Res. 1993;53:230-4.
21. Morse MA, Stoner GD. Cancer chemoprevention: principles and prospects. Carcinogenesis. 1993;14:1737-46.
22. Primanio T, Egner PA, Sutter TR, Kelloff GJ, Roebuck BD, Kensler TW. Intermittent dosing with oltipraz: relationship between chemoprevention of aflatoxin-induced tumorigenesis and induction of glutathione S-transferases. Cancer Res. 1995; 55:4319-24.
23. Rogiers V. Cultures of human hepatocytes in in vitro pharmacotoxicology. In: Rogiers V, Sonck W, Shephard E, Vercruysse A, eds. Human cells in in vitro pharmacotoxicology. Brussels: VUB Press; 1993:77-115.
24. 1 by bacterial recombinant human cytochrome P-450 enzymes. Chem Res Toxicol. 1995;8:219-24.
25. Wattenberg LW. Chemoprevention of cancer. Cancer Res. 1985;45:1-8.
26. Zhang Y, Talalay P. Anticarcinogenic activities of organic isothiocyanates: chemistry and mechanisms. Cancer Res. 1994;54:1976-81.
27. Zhang Y, Talalay P, Cho C, Posner GH. A major inducer of anticarcinogenic protective enzymes from broccoli: isolation and elucidation of structure. Proc Natl Acad Sci USA. 1992;89:2399-403.
28. Zhang Y, Kensler TW, Cho C, Posner GH, Talalay P. Anticarcinogenic activities of sulforaphane and structurally related synthetic norbornyl isothiocyanates. Proc Natl Acad Sci USA. 1994;91:3147-50.