Interaction of oligonucleotide-conjugates with the dipeptide transporter system in Caco-2 cells

Biochemical Pharmacology

Volumn 53, Issue 9, 1997, Pages 1223-1228

  Moore, Vanessa A ^a   Dunnion, Deborah J ^a   Brown, Tom ^b   Irwin, William J ^a   Akhtar, Saghir ^a  

^a ASTON UNIVERSITY   (United Kingdom)

^b UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

Antisense; Caco 2 cells; Dipeptide transporter system; Oligonucleotides; Oral delivery; Phosphorothioates

Indexed keywords

ALPHA TOCOPHEROL; ANTISENSE OLIGODEOXYNUCLEOTIDE; CARRIER PROTEIN; CEPHALOSPORIN; PENICILLIN DERIVATIVE;

HUMAN; HUMAN CELL; INTRACELLULAR TRANSPORT; MOLECULAR STABILITY; PRIORITY JOURNAL; PROTEIN LIPID INTERACTION; PROTEIN NUCLEIC ACID INTERACTION; PROTEIN STABILITY; PROTEIN TARGETING; REVIEW; TRANSPORT KINETICS;

EID: 0030802140     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(96)00862-3     Document Type: Article

References (27)

1. Stein CA and Cheng YC, Antisense oligonucleotides as therapeutic agents - Is the bullet really magical? Science 261: 1004-1012, 1993.
2. Agrawal S and Akhtar S, Advances in antisense efficacy and delivery. Trends Biotechnol 13: 197-199, 1995.
3. Vlassov VV, Yakubo LA, Karamyshev V, Pautova L, Rykova E and Nechaeva M, In vivo pharmacokinetics of oligonucleotides following administration by different routes. In: Delivery Strategies for Antisense Oligodeoxynucleotide Therapeutics (Ed. Akhtar S), pp. 71-83. CRC Press, Boca Raton, FL, 1995.
4. Agrawal S, Zhang X, LU Z, Zhao H, Tamburin JM, Yan J, Cai H, Diaslo RD, Habus I, Jiang Z, Iyer RP, Yu D and Zhang R, Absorption, tissue distribution, and in vivo stability in rats of a hybrid antisense oligonucleotide following oral administration. Biochem Pharmacol 50: 571-576, 1995.
5. Beck GF, Irwin WJ, Nicklin PL and Akhtar S, Interactions of phosphodiester and phosphorothioate oligonucleotides with intestinal epithelial Caco-2 cells. Pharmacol Res 13: 1028-1037, 1996.
6. Akhtar S, Beck GF, Hawley P, Irwin WJ and Gibson I, The influence of polarized epithelial (Caco-2) cell differentiation on the cellular binding of phosphodiester and phosphorothioate oligonucleotides. Antisense Nucleic Acid Drug Dev 6: 197-206, 1996.
7. Nicklin PL, Irwin WJ, Timmins P and Morrison RA, Uptake and transport of the ACE-inhibitor Ceronopril (SQ29852) by monolayers of human intestinal absorptive (Caco-2) cells in vitro. Int J Pharmacol (in press).
8. Dantzig AH, Hoskins J, Tabas LB, Bright S, Shepard RL, Jenkins IL, Duckworth DC, Sportsman IR, Mackensen D, Rosteck, PR, Jr, and Skatrud PL, Association of intestinal peptide-transport with a protein related to the cadherin superfamily. Science 264: 430-433, 1994.
9. Sadee W, Drubbisch V and Amidon GL, Biology of membrane-transport proteins. Pharmacol Res 12: 1823-1837, 1995.
10. Moore VA, Chong S, Dando SA, Lau W, Irwin WJ, Timmins P and Morrsion RA, Systemic evaluation of the structural requirements for the intestinal dipeptide transporter system. Pharmacol Res Suppl 12: S-301, 1995.
11. Will DW and Brown T, Attachment of vitamin E derivatives to oligonucleotides during solid-phase synthesis. Tetrahedron Lett 33: 2729-2732, 1992.
12. MacKellar C, Will DW, Graham D and Brown T, Synthesis and physical-properties of anti-HIV antisense oligonucleotides bearing terminal lipophilic groups. Nucleic Acids Res 20: 3411-3417; 1992.
13. Akhtar S, Basu S, Wickstrom E and Juliano RL, Interactions of antisense DNA analogs with phospholipid-membranes (liposomes). Nucleic Acids Res 19: 5551-5559, 1991.
14. Akhtar S and Juliano RL, Cellular uptake and intracellular fate of antisense oligonucleotides. Trends Cell Biol 2: 139-144, 1992.
15. Kreig AN, Tonkinson J, Matson S, Zhao Q, Saxon M, Zhang L-M, Bhanja U, Yakubov L and Stein CA, Modification of antisense oligodeoxynucleotides by a 5′ cholesteryl moiety increases cellular association and improves efficacy. Proc Natl Acad Sci USA 90: 1048-1052, 1993.
16. de Smidt PC, LeDoan T, de Falco S, van Berkel T, Association of antisense oligonucleotides with lipoproteins prolongs the plasma half-life and modifies the tissue distribution. Nucleic Acids Res 19: 4695-4700, 1991.
17. +/peptide contransporter. J Biol Chem 270: 6456-6463, 1995.
18. Lucas ML, Schneider W, Haberich FJ and Blair JA, Direct measurement by pH-microelectrode of the pH-microclimate in rat proximal jejunum. Proc R Soc Series B 192, 39-48, 1975.
19. Lucas ML and Blair JA, The magnitude and distribution of the acid microclimate in proximal jejunum and its relation to luminal acidification. Proc R Soc Series B 200: 27-41, 1978.
20. Blair JA and Matty AJ, Acid microclimate in intestinal absorption. Clin Gastroenterol 3: 183-198, 1974.
21. Lucas ML, Blair JA, Cooper BT and Cooke WT, Relationship of the acid microclimate in rat and human intestine to malabsorption. Biochem Soc Trans 4: 154-156, 1976.
22. Lucas ML, Cooper BT, Lei FH, Johnson IT, Holmes GKT, Blair JA and Cooke WT, Acid microclimate in coeliac and Crohn's disease: A model for folate malabsorption. Gut 19: 735-742, 1978.
23. Nicklin PL, Irwin WJ, Hassan IF and Mackay M, Proline uptake by monolayers of human intestinal adsorptive (Caco-2) cells in vitro. Biochim Biophys Acta 1104: 283-292, 1992.
24. Marino AM, Chong S, Dando, SA, Kripalani KJ, Bathala MS and Morrsion RA, Distribution of the dipeptide transporter system along the gastrointestinal-tract of rats based on the absorption of a stable and specific probe, SQ-29852. J Pharm Sci 85: 282-286, 1996.
25. Hidalgo IJ and Borchardt RT, Transport of bile-acids in a human intestinal epithelial-cell line, Caco-2. Biochim Biophys Acta 1035: 97-103, 1990.
26. Christensen HN, Role of amino acid transport and counter-transport in nutrition and metabolism. Physiol Rev 70: 43-77, 1990.
27. Hansch C and Leo A, Substituent Constants for Correlation Analysis in Chemistry and Biology. John Wiley & Sons, New York, 1979.