Multipurpose high sensitivity luminescence analyzer (LUANA): Use in gel electrophoresis

BioTechniques

Volumn 20, Issue 4, 1996, Pages 708-713

  Neri, D ^a   Prospero, T ^a   Petrul, H ^a   Winter, G ^a   Browne, M ^b   Vanderpant, L ^c  

^a MRC CENTRE FOR PROTEIN ENGINEERING   (United Kingdom)

^b Kinetic Imaging Limited   (United Kingdom)

^c Digit Pixel Adv Imaging Systems   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BOVINE SERUM ALBUMIN; FLUORESCEIN ISOTHIOCYANATE; PLASMID DNA;

ANALYTIC METHOD; ARTICLE; COMPUTER PROGRAM; FLUORESCENCE; GEL ELECTROPHORESIS; LUMINESCENCE; MOLECULAR BIOLOGY;

CHEMILUMINESCENT MEASUREMENTS; CLONING, MOLECULAR; DNA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT DYES; LASERS; SENSITIVITY AND SPECIFICITY; XENON;

BOVINAE;

EID: 0029990242     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Borjigin, E. and J. Nathans. 1993. Bovine pancreatic trypsin inhibitor-trypsin complex as a detection system for recombinant proteins. Proc. Natl. Acad. Sci. USA 90:337-341.
2. Carr, D.W. and J.D. Scott. 1992. Blotting and band-shifting: techniques for studying protein-protein interactions. Trends Biochem. Sci. 17:246-250.
3. Fried, M. and D.M. Crothers. 1981. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis. Nucleic Acids Res. 9:6505-6525.
4. Garner, M.M. and A. Revzin. 1981. A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: applications to components of the Escherichia coli lactose operon regulatory system. Nucleic Acids Res. 9:3047-3060.
5. Glazer, A.N. and H.S. Rye. 1992. Stable dye-DNA intercalation complexes as reagents for high-sensitivity fluorescence detection. Nature 359:859-861.
6. Hu, G. and B. Vallee. 1994. Gel retardation assay for the interaction of proteins and carbohydrates by FACE. Anal. Biochem. 218:185-191.
7. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
8. Neri, D., C. de Lalla, H. Petrul, P. Neri and G. Winter. 1995. Calmodulin as a versatile tag for antibody fragments. Bio/Technology 13:373-377.
9. Neri, D., M. Momo, T. Prospero and G. Winter. 1995. High-affinity antigen binding by chelating recombinant antibodies (CRAbs). J. Mol. Biol. 246:367-373.
10. Quesada, M.A., H.S. Rye, J.C. Ginrich, A.N. Glazer and R.A. Mathies. 1991. High-sensitivity DNA detection with a laser-excited confocal fluorescence gel scanner. BioTechniques 10:616-625
11. Reed, P.W., J.L. Davies, J.B. Copeman, S.T. Bennett, S.M. Palmer, L.E. Pritchard, S.C.L. Gough, Y. Kawaguchi et al. 1994. Chromosome-specific microsatellite sets for fluorescence-based, semi-automated genome mapping. Nature Genet. 7:390-395.
12. Rydel, T.J, K.G. Ravichandran, A. Tulinskey, W. Bode, R. Huber, C. Roitsch and J.W. Fenton. 1990. The structure of a complex of recombinant hirudin and human a-thrombin. Science 249:277-280.
13. Rye, H.S., M.A. Quesada, K. Peck, R.A. Mathies and A.N. Glazer. 1991. High-sensitivity two-color detection of double-stranded DNA with a confocal fluorescence gel scanner using ethidium homodimer and thiazole orange. Nucleic Acids Res. 19:327-333.
14. Sutherland, J.C., B. Lin, D.C. Monteleone, J. Mugavero, B.M. Sutherland and J. Trunk. 1987. Electronic imaging system for direct and rapid quantitation of fluorescence from electrophoretic gels: application to ethidium bromide-stained DNA. Anal. Biochem. 163:446-457.
15. Vieira, J. and J. Messing. 1987. Production of single-stranded plasmid DNA. Methods Enzymol. 153:3-11.
16. Winter, G., A.D. Griffiths, R.E. Hawkins and H.R. Hoogenboom. 1994. Making antibodies by phage display technology. Annu. Rev. Immunol. 12:433-455.