The bystander effect exerted by tumor cells expressing the herpes simplex virus thymidine kinase (HSVtk) gene is dependent on connexin expression and cell communication via gap junctions

Gene Therapy

Volumn 4, Issue 6, 1997, Pages 577-585

  Vrionis, F D ^a,d   Wu, J K ^a,c   Qi, P ^a   Waltzman, M ^b   Cherington, V ^a,e   Spray, D C ^b  

^a TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^c TUFTS MEDICAL CENTER   (United States)

^d UNIVERSITY OF TENNESSEE COLLEGE OF MEDICINE   (United States)

^e ALG Company   (United States)

Author keywords

Bystander effect; Connexin; Gap junction; Gene therapy; Herpes simplex virus thymidine kinase

Indexed keywords

GAP JUNCTION PROTEIN; THYMIDINE KINASE;

ANIMAL CELL; ARTICLE; CELL COMMUNICATION; CELL DEATH; CELL JUNCTION; CYTOTOXICITY; GENE EXPRESSION; GENETIC TRANSFECTION; HERPES SIMPLEX VIRUS; HUMAN; HUMAN CELL; MOUSE; NEUROBLASTOMA; NONHUMAN; PHEOCHROMOCYTOMA CELL; PRIORITY JOURNAL; RAT; TUMOR CELL; WALKER CARCINOMA;

ANIMALIA; HERPES; SIMPLEXVIRUS;

EID: 0030757097     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3300438     Document Type: Article

References (43)

1. Barba D, Hardin J, Ray J, Gage F. Thymidine kinase-mediated killing of rat brain tumors. J Neurosurg 1993; 79: 729-735.
2. Culver K et al. In vivo gene transfer with retroviral vector-producer cells for treatment of experimental brain tumors Science 1992; 256: 1550-1552.
3. Ezzeddine Z et al. Selective killing of glioma cells in culture and in vivo by retrovirus transfer of herpes simplex virus thymidine kinase gene. New Biol 1991; 3: 608-614.
4. Freeman S et al. The 'bsystander effect': tumor regression when a fraction of the tumor mass is genetically modified. Cancer Res 1993; 53: 5274-5283.
5. Moolten F. Tumor chemosensitivity conferred by inserted herpes thymidine kinase genes: paradigm for prospective cancer control strategy. Cancer Res 1986; 46: 5276-5281.
6. Ram Z et al. In situ retrovirus-mediated gene transfer for the treatment of brain tumors in rats. Cancer Res 1993; 53: 83-88.
7. Vrionis F et al. Tumor cells expressing the herpes simplex virus thymidine kinase gene elicit a more potent bystander cytocidal effect than fibroblast virus producer cells in vitro. J Neurosurg 1995; 83: 698-704.
8. Vrionis F et al. Tumor cells expressing the herpes simplex virus thymidine kinase gene in the treatment of Walker 256 meningeal neoplasia in rats. J Neurosurg 1996; 84: 250-257.
9. Vrionis F et al. Preservation of the bystander cytocidal effect of irradiated herpes simplex virus thymidine kinase (HSVtk) modified tumor cells. J Neuro-oncol 1996; 30: 225-236.
10. Wu J et al. Bystander tumoricidal effect in the treatment of experimental brain tumors. Neurosurgery 1994; 35: 1094-1103.
11. Barba D, Hardin J, Sadelain M, Gage F Development of antitumor immunity following thymidine kinase-mediated killing of experimental brain tumors. Proc Natl Acad Sci USA 1994; 91: 4348-4352.
12. Bi W, Parysek L, Warnick R, Stambrook P. In vitro evidence that metabolic cooperation is responsible for the bystander effect observed with HSVtk retroviral gene therapy. Hum Gene Ther 1993; 4: 725-731.
13. Caruso M et al. Regression of established macroscopic liver metastases after in situ transduction of a suicide gene. Proc Natl Acad Sci USA 1993; 90: 7024-7028.
14. Ram Z et al. The effect of thymidine kinase transduction and ganciclovir therapy on tumor vasculature and growth of 9L gliomas in rats. J Neurosurg 1994; 81: 256-260.
15. Tapscott S et al. Gene therapy of rat 9L gliosarcoma tumors by transduction with selectable genes does not require drug selection. Proc Natl Acad Sci USA 1994; 91: 8185-8189.
16. Kolberg R. The bystander effect in gene therapy: great, but does it work? J NIH Res 1994; 6: 62-64.
17. Elshami A et al. Gap junctions play a role in the 'bystander effect' of the herpes simplex virus thymidine kinase/ganciclovir system in vitro. Hum Gene Ther 1997 (in press)
18. Fick J et al. The extent of heterocellular communication is predictive of bystander tumor cytotoxicity in vitro. Proc Natl Acad Sci USA 1995; 92: 1071-1075.
19. Huber B et al. Metabolism of 5-fluorocytosine to 5-fluorouracil in human colorectal tumor cells transduced with the cytosine deaminase gene: significant antitumor effects when only a small percentage of tumor cells express cytosine deaminase. Proc Natl Acad Sci USA 1994; 91: 8302-8306.
20. Dong Y et al. In vivo replication-deficient adenovirus vector-mediated transduction of the cytosine deaminase gene sensitizes glioma cells to 5-fluorocytosine. Hum Gene Ther 1996; 7: 713-720.
21. Moreno A, Laing J, Beyer E, Spray D. Properties of gap junction channels formed by connexin45 endogenously expressed in human hepatoma (SKHep1) cells. Am J Physiol 1995; 268: C356-C365.
22. Reed K et al. Molecular cloning and functional expression of connexin 37, an endothelial cell gap junction protein. J Clin Invest 1993; 91: 997-1004.
23. Osaki T et al. Gene therapy for carcinoembryonic antigen-producing human lung cancer cells by cell type-specific expression of herpes simplex virus thymidine kinase gene. Cancer Res 1994; 54: 5258-5261.
24. Hamel W, Fick J, Israel M. Treatment of HSVtk expressing cells with ganciclovir induces apoptosis. 11th International Conference on Brain Tumor Research and Therapy, San Francisco, 1995 (Abstr. 108).
25. Ishii H et al. Mechanism of 'bystander effect' killing in the herpes simplex thymidine kinase gene modified tumor system. J Cell Biochem 1994; 18A: 226.
26. Bennett M et al. Gap junctions: new tools, new answers, new questions. Neuron 1991; 6: 305-320.
27. Beyer E, Paul D, Goodenough D. Connexin family of gap junction proteins. J Membrane Biol 1990; 116: 187-194.
28. Pitts J. Cancer gene therapy: a bystander effect using the gap junctional pathway. Molec Carcinogen 1994; 11: 127-130.
29. Spray D, Dermietzel R. X-linked Charcot-Marie-Tooth syndrome and possible gap junction diseases of the nervous system. Trends Neurosci 1995; 18: 256-262.
30. Yamasaki H. Gap junctional intercellular communication and carcinogenesis. Carcinogenesis 1990; 11: 1051-1058.
31. Moreno A, Fishman G, Beyer E, Spray D. Voltage dependent gating and single channel analysis of heterotypic gap junction channels formed of Cx45 and Cx43. Prog Cell Res 1995; 4: 405-408.
32. White T, Paul D, Goodenough D, Bruzzone R. Functional analysis of selective interactions among rodent connexins. Mol Biol Cell 1995; 6: 459-470.
33. Tomasetto C et al. Specificity of gap junction communication among human mammary cells and connexin transfectants in culture. J Cell Biol 1993; 122: 157-167.
34. Tiberghien P et al. Ganciclovir treatment of herpes simplex thymidine kinase-transduced primary T lymphocytes: an approach for specific in vivo donor T cell depletion after bone marrow transplantation. Blood 1994; 84: 1333-1341.
35. Veenstra R et al. Selectivity of connexin-specific gap junctions does not correlate with channel conductance. Circul Res 1995; 77: 1156-1165.
36. Eghbali B, Kessler J, Spray D. Expression of gap junction channels in communication-incompetent cells after stable transfection with cDNA encoding connexin32. Proc Natl Acad Sci USA 1990; 87: 1328-1331.
37. Fishman G, Spray D, Leinwand L. Molecular characterization and functional expression of the human cardiac gap junction channel. J Cell Biol 1990; 111: 589-598.
38. Waltzman M, Spray D. Exogenous expression of connexins for physiological characterization of channel properties: comparison of methods and results. Prog Cell Res 1995; 4: 9-17.
39. Kooistra KL et al. Development of experimental models for meningeal neoplasia using intrathecal injection of 9L gliosarcoma and Walker 256 carcinosarcoma in the rat. Cancer Res 1986; 46: 317-323.
40. Schackert G, Fidler I. Development of in vivo models for studies of brain metastasis. Int J Cancer 1988; 41: 589-594.
41. Liu Q-Y, Summers W. Site-directed mutagenesis of a nucleotide-binding domain in HSV-1 thymidine kinase: effects on catalytic activity. Virology 1988; 163: 638-642
42. Haefliger J-A et al. Four novel members of the connexin family of gap junction proteins. J Biol Chem 1992; 267: 2057-2064.
43. Goliger J, Paul D. Expression of gap junction proteins Cx26, Cx31.1, Cx37, and Cx43 in developing and mature rat epidermis. Develop Dynam 1994; 200: 1-13.