Structure-activity relationship of cantharidin derivatives to protein phosphatases 1, 2A1, and 2B

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 14, 1997, Pages 1833-1836

  Sodeoka, Mikiko ^a   Baba, Yoshiyasu ^a   Kobayashi, Satoko ^a   Hirukawa, Nozomu ^a  

^a SAGAMI CHEMICAL RESEARCH CENTER   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CALCINEURIN; CANTHARIDIN; CANTHARIDIN DERIVATIVE; ISOENZYME; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHOPROTEIN PHOSPHATASE 1; PHOSPHOPROTEIN PHOSPHATASE 2A; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BRAIN; CATTLE; COLORIMETRY; CONTROLLED STUDY; DRUG SYNTHESIS; ENZYME INHIBITION; ENZYME SPECIFICITY; IN VITRO STUDY; NONHUMAN; RABBIT; REACTION ANALYSIS; SKELETAL MUSCLE; STRUCTURE ACTIVITY RELATION;

ANIMALIA; BOS TAURUS; ORYCTOLAGUS CUNICULUS;

EID: 0030742312     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00316-8     Document Type: Article

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19. 1 assays their Ser/Thr Phosphatase Assay Kit 1 was used. Free phosphate ion, released from a substrate phosphopeptide (KRpTIRR), was quantified by colorimetric analysis (630 nm) using the Malachite Green method. p-Nitrophenyl phosphate was used as a substrate for the PP2B assay. Reaction was measured by the absorption of a hydrolyzed product, p-nitrophenol (415 nm).