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1,2H ENDOR experiments of the MoFe protein with deuterated homocitrate revealed the proton hyperfine couplings of the homocitrate are ≤ 1.5 MHz.
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55 See ref 18.
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Mutation at Argα359 to Lysα359 did show the solvent-exchangeable signal in the resting state. Hence, Argα359 is ruled out as the candidate.
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57Fe signals that are difficult to detect by ENDOR, and so this decision calls for confirmatory evidence from Mössbauer spectroscopy.
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