Wild-type and insecticide-resistant homo-oligomeric GABA receptors of Drosophila melanogaster stably expressed in a Drosophila cell line

Neuropharmacology

Volumn 35, Issue 9-10, 1996, Pages 1393-1401

  Buckingham, S D ^a   Matsuda, K ^a   Hosie, A M ^a   Baylis, H A ^a   Squire, M D ^a   Lansdell, S J ^b   Millar, N S ^b   Sattelle, D B ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Dieldrin resistance; Drosophila melanogaster; GABA receptor; Stable (S2 RDL) cell lines; Xenopus oocytes

Indexed keywords

3,3 BIS(TRIFLUOROMETHYL)BICYCLO[2.2.1]HEPTANE 2,2 DICARBONITRILE; 4 AMINOBUTYRIC ACID; 4 AMINOBUTYRIC ACID RECEPTOR; 4 AMINOBUTYRIC ACID RECEPTOR BLOCKING AGENT; 4 AMINOBUTYRIC ACID RECEPTOR STIMULATING AGENT; 4 PROPYL 4' ETHYNYLBICYCLOORTHOBENZOATE; 4' CHLORODIAZEPAM; BARBITURIC ACID DERIVATIVE; BENZODIAZEPINE; BICUCULLINE; CONVULSANT AGENT; DIELDRIN; INSECTICIDE; IONOTROPIC RECEPTOR; PENTOBARBITAL; PHENOBARBITAL; PICROTOXININ; PREGNANE DERIVATIVE; RECEPTOR SUBTYPE; RECEPTOR SUBUNIT; TERT BUTYLBICYCLOPHOSPHOROTHIOATE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CELL LINE; CONCENTRATION RESPONSE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; FEMALE; GENE EXPRESSION; GENETIC TRANSFECTION; INSECTICIDE RESISTANCE; NONHUMAN; OOCYTE; PRIORITY JOURNAL; XENOPUS LAEVIS;

ANIMALS; CELL LINE; DIELDRIN; DROSOPHILA MELANOGASTER; ELECTROPHYSIOLOGY; GABA AGONISTS; INSECTICIDE RESISTANCE; INSECTICIDES; MEMBRANE POTENTIALS; OOCYTES; PATCH-CLAMP TECHNIQUES; RECEPTORS, GABA; TRANSFECTION; XENOPUS LAEVIS;

EID: 0030474063     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(96)00087-1     Document Type: Article

References (34)

1. Aydar E., Taylor A.R., Beadle D.J. and Bermudez I. (1995) A novel electropharmacological profile of insect GABA receptors. J. Physiol. (Lond.) 483: 190-191P.
2. Bai D. (1994) Neurotransmitter receptor subtypes of identified insect neurones. Ph.D thesis, University of Cambridge.
3. A receptor form ion channels with properties of the native receptor. Science. 242:1988;577-579.
4. Buckingham S.D., Hosie A.M., Roush R.T., Sattelle D.B. Actions of agonists and convulsant antagonists on a Drosophila melanogaster GABA receptor (Rdl) homooligomer expressed in Xenopus oocytes. Neurosci. Lett. 183:1994;137-140.
5. Chen R., Belelli D., Lambert J.J., Peters J.A., Reyes A., Lan C.C. Cloning and functional expression of a Drosophila γ-aminobutyric acid receptor. Proc. Natl. Acad. Sci. U.S.A. 91:1994;6069-6073.
6. Chen C.A., Okayama H. High-efficiency transformation of mammalian cells by plasmid DNA. Molec. Cell Biol. 7:1987;2745-2752.
7. 3H]EBOB) in vertebrate brain and insect head. Pestic. Biochem. Physiol. 44:1992;1-8.
8. Cutting G.R., O'Hara B.F., Guggino W.B., Uhl G.R. Identification of 2 members of a novel GABA receptor subunit class highly expressed in the human retina. Am. J. Human Genet. 49:1991;402.
9. Deng Y., Palmer C.J., Casida J.E. House fly brain γ-aminobutyric acid-gated chloride channel: target for multiple classes of insecticides. Pestic. Biochem. Physiol. 41:1991;60-65.
10. Flybase Consortium: Genetic nomenclature guide. (1995) Trends in Genetic. Sci. supplement, 26-29.
11. ffrench-Constant R.H., Mortlock D.P., Schaffer C.D., MacIntyre R.J., Roush R.T. Molecular cloning and transformation of cyclodiene resistance in Drosophila: An invertebrate γ-aminobutyric acid subtype A receptor locus. Proc. Natl. Acad. Sci. U.S.A. 88:1991;7209-7213.
12. ffrench-Constant R.H., Rocheleau T.A., Steichen J.C., Chalmers A.E. A point mutation in a Drosophila GABA receptor confers insecticide resistance. Nature. 363:1993;449-451.
13. Hamill O.P., Marty A., Neher R., Sakmann B., Sigworth F.J. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch. 391:1981;85-110.
14. Hosie A.M., Baylis H.A., Buckingham S.D., Sattelle D.B. Actions of the insecticide, fipronil, on dieldrinsensitive and resistant GABA receptors of Drosophila melanogaster. Br. J. Pharmac. 115:1995;909-912.
15. Hosie A.M., Sattelle D.B. Allosteric modulation of an expressed GABA-gated chloride channel of Drosophila melanogaster. Br. J. Pharmac. 117:1996;1229-1237.
16. Lees G., Beadle D.J., Neumann R., Benson J.A. Responses to GABA by isolated insect neuronal somata - pharmacology and modulation by a benzodiazepine and a barbiturate. Brain Res. 401:1987;267-278.
17. 35S]TBPS in insect CNS. Neurochem. Int. 9:1986;287-293.
18. A receptor channels. Ann. Rev. Neurosci. 17:1994;569-602.
19. Millar N.S., Buckingham S.D., Sattelle D.B. Stable expression of a functional homo-oligomeric Drosophila GABA receptor in a Drosophila cell line. Proc. R. Soc. Lond. B. 258:1994;307-314.
20. A receptor subunit for benzodiazepine pharmacology. Nature. 338:1989;583-585.
21. Rauh J.J., Vasallo J.G., Lummis S.C.R., Wafford K.A., Sattelle D.B. Steroids reveal differences between GABA-gated chloride channels of insects and vertebrates. Molec. Neuropharmac. 3:1993;1-9.
22. Saito M. and Wu C.-F. (1993) Ionic channels in cultured Drosophila neurons. In: Comparative Molecular Neurobiology (Pichon Y., Ed.), pp. 366-389. Birkhäuser, Basel, Switzerland.
23. Sattelle D.B. GABA receptors of insects. Adv. Insect Physiol. 22:1990;1-113.
24. Sattelle D.B., Lummis S.C.R., Wong J.F.H., Rauh J.J. Pharmacology of insect GABA receptors. Neurochem. Res. 16:1991;363-374.
25. Sattelle D.B., Pinnock R.D., Wafford K.A., David J.A. GABA receptors on the cell-body membrane of an identified insect motor neuron. proc. R. Soc. Lond. B. 232:1988;443-456.
26. Shirai Y., Hosie A.M., Buckingham S.D., Holyoke C.W. Jr., Baylis H.A., Sattelle D.B. Actions of picrotoxinin analogues on an expressed, homo-oligomeric GABA receptor of Drosophila melanogaster. Neurosci. Lett. 189:1995;1-4.
27. Sieghart W. Structure and pharmacology of gamma-aminobutyric acid (A) receptor subtypes. Pharmac. Rev. 47:1995;181-234.
28. Smart T.G., Constanti A. Studies on the mechanism of action of picrotoxinin and other convulsants at the crustacean muscle GABA receptor. Proc. R. Soc. Lond. B. 227:1986;191-216.
29. A receptors. Trends Pharmac. Sci. 16:1995;162-168.
30. Taylor A., Bermudez I. and Beadle D.J. (1993) Pharmacology of the GABA receptor of insect central neurones in culture: A patch-clamp study. In: Comparative and Molecular Neurobiology (Pichon Y., Ed.), pp. 146-171. Birkhäuser, Basel, Switzerland.
31. A receptor channels by penicillin. Biophys. J. 59:1991;256a.
32. van der Straten A., Johansen H., Rosenberg M., Sweet R. Introduction and constitutive expression of gene products in cultured Drosophila cells using hygromycin B selection. Methods Molec. Cell Biol. 1:1989;1-8.
33. Zhang H.-G., ffrench-Constant R.H., Jackson M.B. A unique amino acid of the Drosophila GABA receptor with influence on drug sensitivity by two mechanisms. J. Physiol. Lond. 479:1994;65-75.
34. Zhang H.-G., Lee H.J., Rocheleau T., ffrench-Constant R.H., Jackson M.B. Subunit composition determines picrotoxin and bicuculline sensitivity of Drosophila γ-aminobutyric acid receptors. Molec. Pharmac. 48:1995;835-840.