Requirement of an ICE-like protease for induction of apoptosis and ceramide generation by REAPER

Science

Volumn 271, Issue 5250, 1996, Pages 808-810

  Pronk, Gijsbertus J ^a   Ramer, Kevin ^b   Amiri, Payman ^a   Williams, Lewis T ^a,b  

^a NOVARTIS PHARMA AG   (Switzerland)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CERAMIDE; FAS ANTIGEN; INTERLEUKIN 1BETA CONVERTING ENZYME; KETONE DERIVATIVE; PROTEINASE; TUMOR NECROSIS FACTOR RECEPTOR;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL DEATH; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; EMBRYO; EMBRYO DEVELOPMENT; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ANIMALIA; DROSOPHILA MELANOGASTER; MELANOGASTER; VERTEBRATA;

EID: 0030060075     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5250.808     Document Type: Article

References (46)

1. M E. Grether, J. M Abrams, J. Agapite, K. White, H Steller, Genes Dev 9, 1694 (1995).
2. K White et al , Science 264, 677 (1994)
3. J M. Martin and D. R Green, Cell 82. 349 (1995), M. D. Jacobson and G. I Evan, Curr Biol. 4, 337 (1994)
4. J M. Martin and D. R Green, Cell 82. 349 (1995), M. D. Jacobson and G. I Evan, Curr Biol. 4, 337 (1994)
5. J. Yuan, S. Shaham, S. Ledoux, H. M Ellis, H. R. Horvitz, Cell 75, 641 (1993), L. Wang, M. Miura, L Bergeron, H. Zhu, J. Yuan, ibid 78, 739 (1994)
6. J. Yuan, S. Shaham, S. Ledoux, H. M Ellis, H. R. Horvitz, Cell 75, 641 (1993), L. Wang, M. Miura, L Bergeron, H. Zhu, J. Yuan, ibid 78, 739 (1994)
7. D W. Nicholson et al., Nature 376, 37 (1995); M. Tewari et al., Cell 81, 801 (1995); M. Miura, H. Zhu, R. Rotello, E. A. Hartwieg, J. Yuan, ibid. 75, 653 (1993).
8. D W. Nicholson et al., Nature 376, 37 (1995); M. Tewari et al., Cell 81, 801 (1995); M. Miura, H. Zhu, R. Rotello, E. A. Hartwieg, J. Yuan, ibid. 75, 653 (1993).
9. D W. Nicholson et al., Nature 376, 37 (1995); M. Tewari et al., Cell 81, 801 (1995); M. Miura, H. Zhu, R. Rotello, E. A. Hartwieg, J. Yuan, ibid. 75, 653 (1993).
10. S. Kumar, M Kinoshita, M. Noda, N. G. Copeland, N. A Jenkins, Genes Dev 8, 1613 (1994).
11. J. Kamens et al., J. Biol. Chem. 270, 15250 (1995)
12. C. Faucheu et al., EMBO J. 14, 1914 (1995).
13. T. Fernandes-Alnemri, G. Litwack, E. S. Alnemri, J. Biol. Chem. 269, 30761 (1994).
14. N. Boudreau, C. J. Sympson, Z. Werb, M J. Bissell, Science 267, 891 (1995), C. A Ray et al., Cell 69, 597 (1992), N. A Thornberry et al , Biochemistry 33, 3934 (1994).
15. N. Boudreau, C. J. Sympson, Z. Werb, M J. Bissell, Science 267, 891 (1995), C. A Ray et al., Cell 69, 597 (1992), N. A Thornberry et al , Biochemistry 33, 3934 (1994).
16. N. Boudreau, C. J. Sympson, Z. Werb, M J. Bissell, Science 267, 891 (1995), C. A Ray et al., Cell 69, 597 (1992), N. A Thornberry et al , Biochemistry 33, 3934 (1994).
17. M. Enari, H. Hug, S Nagata, Nature 375, 78 (1995); M. Los et al , ibid 376, 37 (1995); M. Tewan and V. M. Dixit, J Biol. Chem. 270, 3255 (1995).
18. M. Enari, H. Hug, S Nagata, Nature 375, 78 (1995); M. Los et al , ibid 376, 37 (1995); M. Tewan and V. M. Dixit, J Biol. Chem. 270, 3255 (1995).
19. M. Enari, H. Hug, S Nagata, Nature 375, 78 (1995); M. Los et al , ibid 376, 37 (1995); M. Tewan and V. M. Dixit, J Biol. Chem. 270, 3255 (1995).
20. J. Schlegel, I. Peters, S Orrenius. FEBS Lett. 364, 139 (1995)
21. R. Kolesnick and D W. Golde, Cell 77, 325 (1994); Y. A. Hannun and L. M Obeid, Trends Biochem Sci. 20, 73 (1995); Y. A Hannun, J. Biol. Chem 269, 3125 (1994).
22. R. Kolesnick and D W. Golde, Cell 77, 325 (1994); Y. A. Hannun and L. M Obeid, Trends Biochem Sci. 20, 73 (1995); Y. A Hannun, J. Biol. Chem 269, 3125 (1994).
23. R. Kolesnick and D W. Golde, Cell 77, 325 (1994); Y. A. Hannun and L. M Obeid, Trends Biochem Sci. 20, 73 (1995); Y. A Hannun, J. Biol. Chem 269, 3125 (1994).
24. W. D. Jarvis et al., Proc. Natl. Acad. Sci. U S.A. 91, 73 (1994); A Haimovitz-Friedman et al , J Exp. Med 180, 525 (1994), M. G. Cifone et al., ibid. 177, 1547 (1994), L M Obeid, C M Linardic, L A Karolak, Y A. Hannun, Science 259, 1769 (1993); G. S Dbaibo, L M. Obeid, Y A. Hannun, J. Biol. Chem. 268, 17762 (1993).
25. W. D. Jarvis et al., Proc. Natl. Acad. Sci. U S.A. 91, 73 (1994); A Haimovitz-Friedman et al , J Exp. Med 180, 525 (1994), M. G. Cifone et al., ibid. 177, 1547 (1994), L M Obeid, C M Linardic, L A Karolak, Y A. Hannun, Science 259, 1769 (1993); G. S Dbaibo, L M. Obeid, Y A. Hannun, J. Biol. Chem. 268, 17762 (1993).
26. W. D. Jarvis et al., Proc. Natl. Acad. Sci. U S.A. 91, 73 (1994); A Haimovitz-Friedman et al , J Exp. Med 180, 525 (1994), M. G. Cifone et al., ibid. 177, 1547 (1994), L M Obeid, C M Linardic, L A Karolak, Y A. Hannun, Science 259, 1769 (1993); G. S Dbaibo, L M. Obeid, Y A. Hannun, J. Biol. Chem. 268, 17762 (1993).
27. W. D. Jarvis et al., Proc. Natl. Acad. Sci. U S.A. 91, 73 (1994); A Haimovitz-Friedman et al , J Exp. Med 180, 525 (1994), M. G. Cifone et al., ibid. 177, 1547 (1994), L M Obeid, C M Linardic, L A Karolak, Y A. Hannun, Science 259, 1769 (1993); G. S Dbaibo, L M. Obeid, Y A. Hannun, J. Biol. Chem. 268, 17762 (1993).
28. W. D. Jarvis et al., Proc. Natl. Acad. Sci. U S.A. 91, 73 (1994); A Haimovitz-Friedman et al , J Exp. Med 180, 525 (1994), M. G. Cifone et al., ibid. 177, 1547 (1994), L M Obeid, C M Linardic, L A Karolak, Y A. Hannun, Science 259, 1769 (1993); G. S Dbaibo, L M. Obeid, Y A. Hannun, J. Biol. Chem. 268, 17762 (1993).
29. J. L Cleveland and J N Ihle, Cell 81, 479 (1995), P Golstein, D. Marguet, V. Depraetere, ibid., p. 185
30. J. L Cleveland and J N Ihle, Cell 81, 479 (1995), P Golstein, D. Marguet, V. Depraetere, ibid., p. 185
31. 4 was added to a final concentration of 0.5 mM, and after 2 hours the percentage of apoptotic cells was determined by examination of at least 200 cells for the presence of plasma membrane blebs
32. 4 was added to a final concentration of 0.5 mM, and after 2 hours the percentage of apoptotic cells was determined by examination of at least 200 cells for the presence of plasma membrane blebs
33. 2-CHPKTGRKSGKYRKP-COOH) (27), cross-linked to keyhole limpet haemocyanin (Animal Pharm Services, Healdsburg, CA). The RPR antiserum was affinity-purified on an RPR peptide column [E Harlow and D. Lane, Antibodies, A Laboratory Manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1988), pp. 313-318].
34. G. J. Pronk, unpublished data.
35. 4 for 90 min Cells were washed once with PBS and were subsequently fixed in 2% glutaraldehyde in PBS for 10 min, after which they were washed three times with PBS After a 30-min incubation at room temperature in PBS. 0.1% Triton X-100, 2% bovine serum albumin (BSA), and 1% normal goat serum, the cells were incubated for 2 hours with preimmune serum or with affinity-purified RPR antiserum in PBS, 0 1% Triton X-100, and 0.2% BSA. Cells were washed three times with PBS and O 1% Triton X-100, and bound RPR antibodies were visualized by incubation with a goat antibody to rabbit immunoglobulin G coupled to fluorescein isothiocyanate.
36. M. J. Smyth and J. A Trapani, Immunol. Today 16, 202 (1995)
37. T Nitatori et al , J. Neurosci 15, 1001 (1995)
38. M. K. Squier, A. C. Miller, A M. Malkinson, J J. Cohen, J, Cell. Physiol. 159, 229 (1994).
39. S. Odake et al., Biochemistry 30, 2217 (1991); D. Rasn̄ick, Anal Biochem. 149, 461 (1985), S. Inoue, S. Bar-Nun, J. Roitelman, R. D Simoni, J. Biol Chem. 266, 13311 (1991).
40. S. Odake et al., Biochemistry 30, 2217 (1991); D. Rasn̄ick, Anal Biochem. 149, 461 (1985), S. Inoue, S. Bar-Nun, J. Roitelman, R. D Simoni, J. Biol Chem. 266, 13311 (1991).
41. S. Odake et al., Biochemistry 30, 2217 (1991); D. Rasn̄ick, Anal Biochem. 149, 461 (1985), S. Inoue, S. Bar-Nun, J. Roitelman, R. D Simoni, J. Biol Chem. 266, 13311 (1991).
42. R. E. Dolle et al., J. Med. Chem. 37, 563 (1994).
43. 32P]ATP (adenosine triphosphate) by diacyl-glyceride kinase relative to total cellular phospholipid [P. P van Veldhoven, T. J. Matthews, D. P. Bolognesi, R M. Bell Biochem. Biophys. Res Commun. 167, 209 (1992)] C2-ceramide and C6-ceramide (Gibco) were disolved in ethanol at a concentration of 10 mM and diluted to a final concentration of 50 μM each in Grace's insect medium (Gibco) containing 0.5% FBS. The medium was sonicated in a bath sonicato for 5 s before being added to the cells At differen time points, the cells were stained with trypan blue and the percent of positive cells was determined The ethanol vehicle alone did not induce cell death
44. R. Bose et al., Cell 82, 405 (1995).
45. Single-letter abbreviations for the amino acid residues are as follows. C, Cys; G, Gly; H, His; K, Lys, P Pro, R, Arg; S, Ser, T, Thr; and Y, Tyr.
46. We thank D. Schneider and J. Weiss for reagents and helpful discussions, and J. Escobedo, K. Giese. S Hamson, M. Kavenaugh, and C. Reinhard for comments on the manuscript. G.J.P. was supported by a stipend from the Netherlands Organization for Scientific Research (NWO) during part of this work.