Homologous expression and purification of mutants of an essential protein by reverse epitope-tagging

Bio/Technology

Volumn 14, Issue 1, 1996, Pages 50-55

  Thomann', Hans Ulrich ^a,b   Ibba, Michael ^a   Hong, Kwang Won ^b   Soil, Dieter ^b  

^a YALE UNIVERSITY   (United States)

^b GENOME THERAPEUTICS CORPORATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; EPITOPE; GLUTAMINE TRANSFER RNA LIGASE; MONOCLONAL ANTIBODY; MUTANT PROTEIN; PROTEIN;

ANTIBODY SPECIFICITY; ARTICLE; BACTERIAL GENETICS; DELETION MUTANT; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; NONHUMAN; STRAIN DIFFERENCE; MUTANT; PURIFICATION;

AMINO ACYL-TRNA SYNTHETASES; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; ARTIFICIAL GENE FUSION; CHROMOSOME DELETION; EPITOPES; ESCHERICHIA COLI; GENES, BACTERIAL; MUTATION; PLASMIDS; SEQUENCE HOMOLOGY, NUCLEIC ACID;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030050934     PISSN: 0733222X     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0196-50     Document Type: Article

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