Amino acid substrate specificity of Escherichia coli phenylalanyl-tRNA synthetase altered by distinct mutations

Journal of Molecular Biology

Volumn 222, Issue 1, 1991, Pages 99-124

  Kast, Peter ^a   Hennecke, Hauke ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

aromatic amino acid binding site; oligonucleotide directed mutagenesis; p fluoro phenylalanine resistance; phenylalanyl tRNA synthetase; pheS gene mutations

Indexed keywords

ESCHERICHIA COLI; STAPHYLOCOCCUS PHAGE 3A;

4 FLUOROPHENYLALANINE; AMINO ACID TRANSFER RNA LIGASE; MUTANT PROTEIN; PHENYLALANINE; PHENYLALANINE DERIVATIVE; PHENYLALANINE TRANSFER RNA;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINOACYLATION; ARTICLE; BINDING SITE; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

ACYLATION; AMINO ACID SEQUENCE; AMINO ACIDS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHENYLALANINE-TRNA LIGASE; SEQUENCE ALIGNMENT; SUBSTRATE SPECIFICITY; SUPPORT, NON-U.S. GOV'T;

EID: 0026015417     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-2836(91)90740-W     Document Type: Article

References (136)

1. Phenylalanyl-tRNA synthetase from Thermus thermophilus HB8. Purification and properties of the crystallizing enzyme
2. Tyr426 of the Escherichia coli asparaginyl-tRNA synthetase, an amino acid in a C-terminal conserved motif, is involved in ATP binding
3. Linkage map of Escherichia coli K-12
4. Phe phenylalanylation are concerted
5. Yeast phenylalanyl-tRNA synthetase. Affinity and photoaffinity labelling of the stereospecific binding sites
6. Mean and Equilibrium Molecular Structures of Methane and Deuteromethane as Determined by Electron Diffraction
7. Chemical modifications of a cysteinyl residue introduced in the binding site of carboxypeptidase Y by site-directed mutagenesis
8. Structural homology in the amino-terminal domains of two aminoacyl-tRNA synthetases
9. Relation between Subunit Structure and Temperature-Sensitivity of Mutant Phenylalanyl RNA Synthetases of Escherichia coli
10. Genetic Mapping of Phenylalanyl-sRNA Synthetase in Escherichia coli
11. van der Waals' volumes and radii
12. Theories of enzyme specificity and their application to proteases and aminoacyl-transfer RNA synthetases
13. A complementation analysis of the restriction and modification of DNA in Escherichia coli
14. Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes
15. Erratum
16. Crystal structure of a deletion mutant of a tyrosyl-tRNA synthetase complexed with tyrosine
17. Structure of tyrosyl-tRNA synthetase refined at 2.3 Å resolution. Interaction of the enzyme with the tyrosyl adenylate intermediate
18. Formation of aromatic amino acid pools in Escherichia coli K-12
19. Crystallographic study at 2.5 Å resolution of the interaction of methionyl-tRNA synthetase from Escherichia coli with ATP
20. Understanding structural relationships in proteins of unsolved three-dimensional structure
21. Aromatic-aromatic interaction: A mechanism of protein structure stabilization
22. Dimerization energetics of benzene and aromatic amino acid side-chains
23. “Western blotting”: Eletrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographie detection with antibody and radioiodinated protien A
24. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid
25. Preliminary crystallographic study of the phenylalanyl-tRNA synthetase from Thermus thermophilus HB8
26. Principles that determine the structure of proteins
27. The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: Effects on cell growth and induction of met regulon expression
28. Genes for the α and β subunits of the phenylalanyl-transfer ribonucleic acid synthetase of Escherichia coli
29. Automated DNA sequence analysis
30. Redesigning trypsin: Alteration of substrate specificity
31. A second class of synthetase structure revealed by X-ray analysis of Escherichia coli seryl-tRNA synthetase at 2.5 Å
32. Establishing homologies in protein sequences
33. Low-resolution structure of the tetrameric phenylalanyl-tRNA synthetase from Escherichia coli. A neutron small-angle scattering study of hybrids composed of protonated and deuterated protomers
34. Polyprotein Gene Expression: Generation of Diversity of Neuroendocrine Peptides
35. Protein and nucleic acid synthesis in two mutants of Escherichia coli with temperature-sensitive aminoacyl ribonucleic acid synthetases
36. Regulation of formation of threonyl-tRNA synthetase, phenylalanyl-tRNA synthetase and protein synthesis initiation factor 3 from Escherichia coli in vivo and in vitro
37. Aromaticity at position 37 in human epidermal growth factor is not obligatory for activity
38. Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs
39. Aspartyl-tRNA synthetase from Escherichia coli: Cloning and characterisation of the gene, homologies of its translated amino acid sequence with asparaginyl- and lysyl-tRNA synthetases
40. Probing steric and Hydrophobic effects on enzyme-substrate interactions by protein engineering
41. Protein and ribonucleic acid synthesis in a mutant of Escherichia coli with an altered aminoacyl ribonucleic acid synthetase
42. The aminoacyladenylate mechanism in the aminoacylation reaction of yeast phenylalanyl-tRNA synthetase
43. Phe binding domain in the tetrameric structure of cytoplasmic phenylalanyl-tRNA synthetase from baker's yeast
44. The molecular weight and subunit composition of phenylalanyl-tRNA synthetase from Escherichia coli K-12
45. Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20
46. Hydrogen bonding and biological specificity analysed by protein engineering
47. Reconstruction by site-directed mutagenesis of the transition state for the activation of tyrosine by the tyrosyl-tRNA synthetase: A mobile loop envelopes the transition state in an induced-fit mechanism
48. Mechanisms of aminoacyl-tRNA synthetases: A critical consideration of recent results
49. 2)
50. Evolutionary aspects of accuracy of phenylalanyl-tRNA synthetase. A comparative study with enzymes from Escherichia coli, Saccharomyces cerevisiae, Neurospora crassa, and turkey liver using phenylalanine analogues
51. Purification by salting-out chromatography and properties of phenylalanyl-tRNA synthetase from turkey liver
52. Structure and evolution of a group of related aminoacyl-tRNA synthetases
53. Production of abnormal proteins in E. coli stimulates transcription of lon and other heat shock genes
54. Escherichia coli mutants overproducing phenylalanyl- and threonyl-tRNA synthetase.
55. Molecular basis of thermostability in the lysozyme from bacteriophage T4
56. An interactive computer graphics study of thermolysin-catalyzed peptide cleavage and inhibi-tion by N-carboxymethyl dipeptides
57. l-Phenylalanyl-tRNA synthetase of Escherichia coli K-10. A reinvestigation of molecular weight and subunit structure
58. Use of mutant enzymes to demonstrate the presence of two active sites on phenylalanyl-tRNA synthetase from Escherichia coli
59. Altered α subunits in phenylalanyl-tRNA synthetases from p-fluorophenylalanine-resistant strains of Escherichia coli
60. A specialized transducing λ phage carrying the Escherichia coli genes for phenylalanyl-tRNA synthetase
61. Immunochemical studies on phenylalanyl-tRNA synthetase from Escherichia coli
62. A novel cloning vector for the direct selection of recombinant DNA in E. coli
63. CLUSTAL: A package for performing multiple sequence alignment on a microcomputer
64. Phe to phenylalanyl-tRNA synthetase of Escherichia coli K10
65. Applications of amino acid analogs for studying co- and posttranslational modifications of proteins
66. Met
67. Sequence similarities among the family of aminoacyl-tRNA synthetases
68. Phe
69. Affinity labelling of aminoacyl-tRNA synthetases with adenosine triphosphopyridoxal: Probing the Lys-Met-Ser-Lys-Ser signature sequence as the ATP-binding site in Escherichia coli methionyl- and valyl-tRNA synthetases
70. π-π Interactions: the geometry and energetics of phenylalanine-phenylalanine interactions in proteins
71. Experimental proof for the misactivation of amino acids by aniinoacyl-tRNA synthetases
72. Convenient construction of strains useful for transducing recA mutations with bacteriophage P1
73. Polypeptide Growth Factors
74. Two mutations in the dispensable part of alanine tRNA synthetase which affect the catalytic activity
75. Phenylalanyl-tRNA synthetase from E. coli MRE-600: Analysis of the active site distribution on the enzyme subunits by affinity labelling
76. Isolation and characterization of the yeast gene coding for the α subunit of mitochondrial phenylalanyl-tRNA synthetase
77. Nonprotein amino acid furanomycin, unlike isoleucine in chemical structure, is changed to isoleucine tRNA by isoleucyl-tRNA synthetase and incorporated into protein
78. Biosynthesis of a protein containing a nonprotein amino acid by Escherichia coli: l-2-aminohexanoic acid at position 21 in human epidermal growth factor
79. The subunit structure of phenylalanyl-tRNA synthetase of Escherichia coli
80. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
81. Transition-state stabilization in the mechanism of tyrosyl-tRNA synthetase revealed by protein engineering
82. Protein measurement with the folin phenol reagent
83. lac, Repressor: 3-fluorotyrosine substitution for nuclear magnetic resonance studies
84. Neuropeptides: multiple molecular forms, metabolic pathways, and receptors
85. Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding
86. Sequence of the Escherichia coli pheST operon and identification of the himA gene
87. Methionyl-tRNA synthetase from E. coli —a review
88. Engineering protein thermal stability
89. 1H bond
90. Effects of proline analogues on the formation of alkaline phosphatase in Escherichia coli
91. Glycyl-tRNA synthetase of Escherichia coli: Immunological homology with phenylalanyl-tRNA synthetase
92. Inhibition of restriction endonuclease NciI cleavage by phosphorothioate groups and its application to oligonucleotide-directed mutagenesis
93. Roles of amino acid activating enzymes in cellular physiology
94. Determination of total protein
95. In vitro incorporation of L-canavanine into vitellogenin of the fat body of the migratory locust Locusta migratoria migratorioides.
96. Escherichia coli phenylalanyl-tRNA synthetase operon: Characterization of mutations isolated on multicopy plasmids
97. Physical localisation and cloning of the structural gene for E. coli initiation factor IF3 from a group of genes concerned with translation
98. Study of the arrangement of the functional domains along the yeast cytoplasmic aspartyl-tRNA synthetase
99. Phe. Isolation and sequence of the labelled peptide
100. The effect of amino acid analogues on growth and protein synthesis in microorganisms
101. Hydrophobicity of amino acid residues in globular proteins
102. Chemical and biological evolution of a nucleotide-binding protein
103. Gln and ATP at 2.8 Å resolution
104. Asp and aspartyl-tRNA synthetase
105. DNA sequencing with chain-terminating inhibitors
106. Structure and expression of the genes encoding the α and β subunits of yeast phenylalanyl-tRNA synthetase
107. Construction of a FRS1-FRS2 operon encoding the structural genes for the α and β subunits of yeast phenylalanyl-tRNA synthetase and its use in deletion analysis
108. Phe acceptor end with the synthetase involves a sequence common to yeast and Escherichia coli phenylalanyl-tRNA synthetases
109. Phenylalanyl transfer ribonucleic acid synthetase from Escherichia coli. Analysis of the phenylalanine binding site
110. Aminoacyl-tRNA synthetases: general scheme of structure-function relationships in the polypeptides and recognition of transfer RNAs
111. Aminoacyl-tRNA synthetases: general features and recognition of transfer RNAs
112. Synthesis of Nonproteinogenic (R)- or (S)-Amino Acids Analogues of Phenylalanine, Isotopically Labelled and Cyclic Amino Acids fromtert-Butyl 2-(tert-Butyl)-3-methyl-4-oxo-1-imidazolidinecarboxylate (Boc-BMI)
113. Aromatic-aromatic interactions and protein stability. Investigation by double-mutant cycles
114. Amino acid analogues
115. λ ZAP: a bacteriophage λ expression vector with in vivo excision properties
116. Fluorescence detection in automated DNA sequence analysis
117. Aminoacyl-tRNA synthetases
118. Detection of specific sequences among DNA fragments separated by gel electrophoresis
119. Escherichia coli phenylalanyl-tRNA synthetase operon is controlled by attenuation in vivo
120. Attenuation control of the Escherichia coli phenylalanyl-tRNA synthetase operon
121. Fluorotyrosine alkaline phosphatase from Escherichia coli: Preparation, properties, and fluorine-19 nuclear magnetic resonance spectrum
122. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase
123. A mutation in the small (α) subunit of glycyl-tRNA synthetase affects amino acid activation and subunit association parameters
124. Primary structures of and genes for new ribosomal proteins A and B in Escherichia coli
125. Prediction of a common structural domain in aminoacyl-tRNA synthetases through use of a new pattern-directed inference system
126. tRNA-like structures tag the 3′ ends of genomic RNA molecules for replication: Implications for the origin of protein synthesis
127. Recruitment of substrate-specificity properties from one enzyme into a related one by protein engineering
128. Designing substrate specificity by protein engineering of electrostatic interactions
129. Incorporation of modified amino acids into proteins in vivo
130. Improved M13 phage cloning vectors and host strains: Nucleotide sequence of the M13mp18 and pUC19 vectors