Intracellular expression of cellular eIF-5A mutants inhibits HIV-1 replication in human T cells: A feasibility study

Human Gene Therapy

Volumn 7, Issue 15, 1996, Pages 1861-1869

  Junker, Uwe ^a   Bevec, Dorian ^a   Barske, Carmen ^a,b   Kalfoglou, Creton ^a   Escaich, Sonia ^a,c   Dobrovnik, Marika ^a   Hauber, Joachim ^a,d   Böhnlein, Ernst ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

^b Sandoz Pharma Ltd   (Switzerland)

^c SANOFI   (France)

^d UNIVERSITY HOSPITAL ERLANGEN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; INITIATION FACTOR; MUTANT PROTEIN; VIRUS VECTOR;

ANTIVIRAL ACTIVITY; ARTICLE; CELL METABOLISM; CELL MUTANT; CELL POPULATION; CELL PROLIFERATION; CONTROLLED STUDY; GENE THERAPY; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LEUKEMIA VIRUS; LONG TERMINAL REPEAT; MODEL; PERIPHERAL LYMPHOCYTE; PROTEIN EXPRESSION; RETROVIRUS; T LYMPHOCYTE; VIRUS INHIBITION; VIRUS REPLICATION;

EUKARYOTA; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; MURINAE; MURINE LEUKEMIA VIRUS; UNIDENTIFIED RETROVIRUS;

EID: 0029857203     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1996.7.15-1861     Document Type: Article

References (35)

1. ADAM, M.A., RAMESH, N., MILLER, A.D., and OSBORNE, W.A. (1991). Internal initiation of translation in retroviral vectors carrying picornavirus 5′ non-translated regions. J. Virol. 65, 4985-4990.
2. BAHNER, I., ZHOU, C., YU, X.-J., HAO, Q.-L., GUATELLI, J.C., and KOHN, D.B. (1993). Immunodeficiency virus type I genes expressed by retroviral vectors in human T lymphocytes. J. Virol. 67, 3199-3207.
3. BECK, E., LUDWIG, G., AUERSWALD, E.A., REISS, B., and SCHALLER, H. (1982). Nucleotide sequence and exact localization of the neomycin-phosphotransferase gene from transposon Tn5. Gene 19, 327-336.
4. BEVEC, D., DOBROVNIK, M., HAUBER, J., and BÖHNLEIN, E. (1992). Inhibition of human immunodeficiency virus type 1 replication in human T cells by retroviral-mediated gene transfer of a dominant-negative Rev trans-activator. Proc. Natl. Acad. Sci. USA 89, 9870-9874.
5. BEVEC, D., KLIER, H., HOLTER, W., TSCHACHLER, E., VALENT, P., LOTTSPEICH, F., BAUMRUKER, T., and HAUBER, J. (1994). Induced gene expression of the hypusine-containing protein eukaryotic initiation factor 5A in activated human T lymphocytes. Proc. Natl. Acad. Sci. USA 91, 10829-10833.
6. BEVEC, D., JAKSCHE, H., OFT, M., WÖHL, T., HIMMELSPACH, M., PACHER, A., SCHEBESTA, M., KOETTNITZ, K., DOBROVNIK, M., CSONGA, R., LOTTSPEICH, F., and HAUBER, J. (1996). Inhibition of HIV-1 replication in lymphocytes by mutants of the Rev cofactor eIF-5A. Science 271, 1858-1860.
7. BOGERD, H.P., FRIDELL, R.A., MADORE, S., and CULLEN, B.R. (1995). Identification of a novel cellular cofactor for the Rev/Rex class of retroviral regulatory proteins. Cell 82, 485-494.
8. CHIRGWIN, J.M., PRZYBALA, A.E., MACDONALD, R.J., and RUTTER, W.J. (1979). Isolation of biological active RNA from sources enriched in ribonuclease. Biochemistry 18, 5294-5299.
9. CULLEN, B.R. (1992). Mechanism of action of regulatory proteins encoded by complex retroviruses. Microbiol. Rev. 56, 375-394.
10. DUKE, G.M., HOFFMANN, M.A., and PALMENBERG, A.C. (1992). Sequence and structural elements that contribute to efficient encephalomyocarditis virus RNA translation. J. Virol. 66, 1602-1609.
11. ERCOLANI, L., FLORENCE, B., DENARO, M., and ALEXANDER, M. (1988). Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene. J. Biol. Chem. 263, 15335-15341.
12. ESCAICH, S., KALFOGLOU, C., PLAVEC, I., KAUSHAL, S., MOSCA, J.D., and BÖHNLEIN, E. (1995). RevM10-mediated inhibition of HIV-1 replication in chronically infected T cells. Hum. Gene Ther. 6, 625-634.
13. FORESTELL, S.P., BÖHNLEIN, E., and RIGG, R.J. (1995). Retroviral end-point titer is not predictive of gene transfer efficiency: Implications for vector production. Gene Ther. 2, 723-730.
14. FOX, B.A., WOFFENDIN, C., YANG, Z.-Y., SAN, H., RANGA, U., GORDON, D., OSTERHOLZER, J., and NABEL, G.J. (1995). Genetic modification of human peripheral blood lymphocytes with a transdominant negative form of rev: Safety and toxicity. Hum. Gene Ther. 6, 997-1004.
15. FRITZ, C.C., ZAPP, M.L., and GREEN, M.R. (1995). A human nucleoporin-like protein that specifically interacts with HIV Rev. Nature 376, 530-533.
16. FURUTA, R.A., KUBOTA, S., MAKI, M., MIYAZAKI, Y., HATTORI, T., and HATANAKA, M. (1995). Use of human immunodeficiency virus type 1 rev mutant without nucleolar dysfunction as a candidate for potential AIDS therapy. J. Virol. 69, 1591-1599.
17. JUNKER, U., BÖHNLEIN, E., and VERES, G. (1995). Genetic instability of a MoMLV-based antisense double-copy retroviral vector designed for HIV-1 gene therapy. Gene Ther. 2, 639-646.
18. KOETTNITZ, K., KAPPEL, B., BAUMRUKER, T., HAUBER, J., and BEVEC, D. (1994). The genomic structure encoding human initiation factor eIF-5A. Gene 144, 249-252.
19. LEVY, J.A., HOFFMANN, A.D., KRAMER, S.M., LANDIS, J.A., SHIMABUKURO, J.M., and OSHIRO, L.S. (1984). Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS. Science 225, 840-842.
20. MALIM, M., HAUBER, J., LE, S.Y., MAIZEL, J., and CULLEN, B.R. (1989). The HIV-1 rev trans-activator acts through a structured target sequence to activate nuclear export of unspliced viral mRNA. Nature 338, 254-257.
21. MALIM, M.H., MCCARN, D.F., TILEY, L.S., and CULLEN, B.R. (1991). Mutational definition of the human immunodeficiency virus type 1 Rev activation domain. J. Virol. 65, 4248-4254.
22. MALIM, M.H., FREIMUTH, W.W., LIU, J., BOYLE, T.J., LYLERLY, H.K., CULLEN, B.R., and NABEL, G.J. (1992). Stable expression of transdominant rev protein in human T cells inhibits human immunodeficiency virus replication. J. Exp. Med. 176, 1197-1201.
23. MILLER, A.D., and BUTTIMORE, C. (1986). Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production. Mol. Cell. Biol. 6, 2895-2902.
24. MILLER, A.D., and ROSMAN, G.J. (1989). Improved retroviral vectors for gene transfer and expression. Bio/Technology 7, 980-988.
25. MOSMAN, T. (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth. 65, 55-63.
26. NABEL, G.J., FOX, B.A., POST, L., THOMPSON, C.B., and WOFFENDIN, C. (1994). A molecular genetic intervention for AIDS - effects of a transdominant negative form of Rev. Hum. Gene Ther. 5, 79-92.
27. NARA, P.L., and FISCHINGER, P.J. (1988). Quantitative infectivity assay for HIV-1 and -2. Nature 332, 469-470.
28. PEAR, W., NOLAN, G., SCOTT, M., and BALTIMORE, D. (1993). Production of high-titer helper-free retroviruses by transient transfection. Proc. Natl. Acad. Sci. USA 90, 8392-8396.
29. RATNER, L., FISHER, A., JAGODZINSKI, L.L., MITSUYA, H., LIOU, R.-S., GALLO, R.C., and WONG-STAAL, F. (1987). Complete nucleotide sequences of functional clones of the AIDS virus. AIDS Res. Hum. Retrov. 3, 57-69.
30. RIDDELL, S.R., ELLIOTT, M., LEWINSOHN, D.A., GILBERT, M.J., WILSON, L., MANLEY, S.A., LUPTON, S.D., OVERELL, R.W., REYNOLDS, T.C., COREY, L., and GREENBERG, P.D. (1996). T-cell mediated rejection of gene-modified HIV-specific cytotoxic T lymphocytes in HIV-infected patients. Nature Med. 2, 216-223.
31. RUHL, M., HIMMELSPACH, M., BAHR, G.M., HAMMERSCHMID, F., JAKSCHE, H., WOLFF, B., ASCHAUER, H., FARRINGTON, G.K., PROBST, H., BEVEC, D., and HAUBER, J. (1993). Eukaryotic initiation factor 5A is a cellular target of the human immunodeficiency virus type 1 Rev activation domain mediating transactivation. J. Cell Biol. 123, 1309-1320.
32. SAMBROOK, J., FRITSCH, E.F., and MANIATIS, T. (1989). In Molecular Cloning: A Laboratory Manual. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY).
33. STUTZ, F., NEVILLE, M., and ROSBASH, M. (1995). Identification of a novel nuclear pore-associated protein as a functional target of the HIV-1 rev protein in yeast. Cell 82, 495-506.
34. TAGAWA, M., NAKAUCHI, H., HERZENBERGER, L.A., and NOLAN, G.P. (1986). Formal proof that different-size Lyt-2 polypeptides arise from differential splicing and post-transcriptional regulation. Proc. Natl. Acad. Sci. USA 83, 3422-3426.
35. WOFFENDIN, C., RANGA, U., YANG, Z.-Y., XU, L., and NABEL, G.J. (1996). Expression of a protective gene prolongs survival of T cells in human immunodeficiency virus-infected patients. Proc. Natl. Acad. Sci. USA 93, 2889-2894.