Quantifying apoptosis in banked human brains using flow cytometry

Journal of Neuropathology and Experimental Neurology

Volumn 55, Issue 11, 1996, Pages 1164-1172

  Olano, Juan P ^a   Wolf, Dwayne ^a   Keherly, Michael ^a   Gelman, Benjamin B ^a  

^a University of Texas Medical Branch School of Medicine   (United States)

Author keywords

AIDS; Brain banking; Cell death; Isopyknic centrifugation; Nuclear isolation; TUNEL

Indexed keywords

APOPTOSIS; ARTICLE; BRAIN DEVELOPMENT; BRAIN TUMOR; CELL DEATH; DEGENERATIVE DISEASE; DNA DEGRADATION; FLOW CYTOMETRY; HUMAN; HUMAN TISSUE; PATHOPHYSIOLOGY; PRIORITY JOURNAL;

EID: 0029807007     PISSN: 00223069     EISSN: None     Source Type: Journal    
DOI: 10.1097/00005072-199611000-00008     Document Type: Article

References (32)

1. Kerr JFR, Wyllie AH, Currie AR. Apoptosis: A basic biological phenomenon with wide ranging implications in tissue kinetics. Br J Cancer 1972;26:239-57
2. White E. Life, death and the pursuit of apoptosis. Genes and development 1996;10:1-15
3. Carson DA, Ribeiro JM. Apoptosis and disease. Lancet 1993;341: 1251-55
4. Stewart BW. Mechanisms of Apoptosis: Integration of genetic, biochemical and cellular indicators. J Nat Cancer Inst 1994;86:1286-95
5. Vaux DL. Toward an understanding of the molecular mechanisms of physiological cell death. Proc Natl Acad Sci USA 1993;90:786-89
6. Raff MC, Barres BA, Burne JF, Colles HS, Ishizaki Y, Jacobson MD. Programmed cell death and the control of cell survival: Lessons from the nervous system. Science 1993;262:695-99
7. Gray F, Scaravilli F, Everall I. Chretien F, et al. Neuropathology of early HIV-1 infection. Brain Pathology 1996;6:1-15
8. Petito CK, Roberts B. Evidence of apoptotic cell death in HIV encephalitis. Am J Pathol 1995;146:1121-30
9. Sharer LR, Krajewskj S, James H, Epstein LG, Reed JC, Gelbard HA. Apoptosis related gene products in brains of pediatric patients with HIV encephalitis, [abstract]. J Neuropathol Exp Neurol 1996;55:663
10. Linnik MD, Zobrist RH, Hatfield MD. Evidence supporting a role for programmed cell death in focal cerebral ischemia in rats. Stroke 1993;24:2002-6
11. Bonetti B, Raine CS. Bcl-2 and Fas system molecule expression in multiple sclerosis lesions, [abstract]. J Neuropathol Exp Neurol 1996;55:641
12. Sgonc R, Wick G. Methods for the detection of apoptosis. Int Arch Allergy Immunol 1994;105:327-32
13. Gorczyca W, Gong J, Darzynkiewicz Z. Detection of DNA strand breaks in individual apoptotic cells by the in situ terminal deoxynucleotidyl transferase and nick translation assays. Cancer Res 1993;53:1945-51
14. Gavrieli Y, Sherman Y, Ben-Sasson SA. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 1992;119:493-501
15. Olano JP, Wolf DA, Keherly MJ, Gelman BB. Terminal deoxynucleotidyl transferase-labeled DNA strand breaks in isolated brain nuclei detected using flow cytometry. [abstract]. J Neuropathol Exp Neurol 1996;55:616
16. Yanagihara T. Experimental stroke in gerbils: Effects on translation and transcription. Brain Research 1978;158:435-44
17. Yanagihara T. Phosphorylation of chromatin proteins in cerebral anoxia and ischemia. J Neurochem 1980;35:1209-15
18. Dolzhanskiy A, Basch RS. Flow cytometric determination of apoptosis in heterogenous cell populations. J Immunol Meth 1995;180: 131-40
19. Migheli A, Cavalla P, Marino S, Schiffer D. A study of apoptosis in normal and pathologic nervous tissue after in situ end labeling of DNA strand breaks. J Neuropathol Exp Neurol 1994;53:606-16
20. Darzynkiewicz Z, Bruno S, Del Bino G, Gorczyca W, Hotz MA, Lassota P, Traganos F. Features of apoptotic cells measured by flow cytometry. Cytometry 1992;13:795-808
21. Achim CL, Wang R, Miners DK, Wiley CA. Brain viral burden in HIV infection. J Neuropathol Exp Neurol 1994;53:284-94
22. Rösl F. A simple method for detection of apoptosis in human cells. Nucleic acids Res 1992;20:5243
23. Gold R, Schmidt M, Rothe G, et al. Application of in-situ nick translation to cell culture systems and tissue sections. J Histochem Cytochem 1993;41:1023-30
24. Melamed MR, Lindmo T, Mendelsohn ML. Flow cytometry and sorting, 2nd ed. New York: Wiley-Liss, 1990
25. Dive C, Gregory CD, Phipps DJ, Evans DL, Milner AE, Wyllie AH. Analysis and discrimination of necrosis and apoptosis (programmed cell death) by multiparameter flow cytometry. Biochim Biophys Acta 1992;1133:275-85
26. Lassmann H, Bancher C, Breitschhopf H, Wegiel, Bobinsky M. Cell death in Alzheimer's disease evaluated by DNA fragmentation in situ. Acta Neuropathol 1995;89:35-41
27. Dickson D. Apoptosis in the brain: Physiology and pathology. Am J Pathol 1995;146:1040-44
28. Götz C, Montenarh M. p53: DNA cleavage, DNA repair and apoptosis. Rev Physiol Biochem Pharmacol 1995;127:65-85
29. Boise LH, Gonzalez-Garcia M, Postema CE, et al. bclx, a bcl-2 related gene that functions as a dominant regulator of apoptotic cell death. Cell 1993;74:597-608
30. Telford WG, King LE, Fraker PJ. Comparative evaluation of several DNA binding dyes in the detection of apoptosis-associated chromatin degradation by flow cytometry. Cytometry 1992;13:137-43
31. Tounekti O, Belehradek J Jr, Mir LM. Relationships between DNA fragmentation, chromatin condensation and changes in flow cytometry profiles detected during apoptosis. Exp Cell Res 1995;217: 506-16
32. Gorczyca W, Bigman K, Mittleman A, et al. Induction of DNA strand breaks associated with apoptosis during treatment of leukemia. Leukemia 1993;7:659-70