Expression and deletion mutagenesis of tryptophan hydroxylase fusion proteins: Delineation of the enzyme catalytic core

Journal of Neurochemistry

Volumn 67, Issue 3, 1996, Pages 917-926

  D'Sa, Carrol M ^a   Arthur Jr , Robert E ^a   Kuhn, Donald M ^a,b  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b Metropol Center for High Technology   (United States)

Author keywords

Brain; Catalytic core; Glutathione S transferase fusion protein; Non heme iron; Regulatory domain; Tryptophan hydroxylase

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; RECOMBINANT PROTEIN; TRYPTOPHAN HYDROXYLASE;

AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; BRAIN; CATALYSIS; DELETION MUTANT; ENZYME KINETICS; ENZYME REGULATION; ESCHERICHIA COLI; GENE EXPRESSION; MALE; MICHAELIS MENTEN KINETICS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; RAT;

ANIMALIA; ESCHERICHIA COLI;

EID: 0029800076     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1996.67030917.x     Document Type: Article

References (54)

1. Bradford M. M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
2. Campbell D. G., Hardie D. G., and Vulliet P. R. (1986) Identification of four phosphorylation sites in the N-terminal region of tyrosine hydroxylase. J. Biol. Chem. 261, 10489-10492.
3. Cash C. D., Vayer P., Mandel P., and Maitre M. (1985) Tryptophan 5-hydroxylase: rapid purification from whole rat brain and production of a specific antiserum. Eur. J. Biochem. 149, 239-245.
4. Daemon M. C., Guibert B., Leviel V., Ehret M., Maitre M., and Mallet J. (1988) Sequence of two mRNAs encoding active rat tryptophan hydroxylase. J. Neurochem. 51, 312-316.
5. Daubner S. C., Lohse D. L., and Fitzpatrick P. F. (1993) Expression and characterization of catalytic and regulatory domains of rat tyrosine hydroxylase. Protein Sci. 2, 1452-1460.
6. Dickson P. W., Jennings I. G., and Cotton R. G. H. (1994) Delineation of the catalytic core of phenylalanine hydroxylase and identification of glutamate 286 as a critical residue for pterin function. J. Biol. Chem. 32, 20369-20375.
7. Dix T. A., Kuhn D. M., and Benkovic S. J. (1987) Mechanism of oxygen activation by tyrosine hydroxylase. Biochemistry 26, 3354-3361.
8. Doskeland A. P., Martinez A., Knappskog P. M., and Flatmark T. (1996) Phosphorylation of recombinant phenylalanine hydroxylase: effect on catalytic activity, substrate activation and protection against non-specific cleavage of the fusion protein by restriction protease. Biochem. J. 313, 409-414.
9. D'Sa C., Arthur R. E., and Kuhn D. M. (1996a) Purification of tryptophan hydroxylase by affinity chromatography on calmodulin sepharose. J. Neurosci. Methods (in press).
10. D'Sa C. M., Arthur R. E. Jr., States J. C., and Kuhn D. M. (1996b) Tryptophan hydroxylase: cloning and expression of the rat brain enzyme in mammalian cells. J. Neurochem. 67, 900-906.
11. 2+/calmodulin-dependent protein kinase. J. Neurochem. 52, 1886-1891.
12. Fisher D. B. and Kaufman S. (1973) The stimulation of rat liver phenylalanine hydroxylase by lysolecithin and α-chymotrypsin. J. Biol Chem. 248, 4345-4352.
13. Furukawa Y., Ikuta N., Omata S., Yamauchi T., Isobe T., and Ichimura T. (1993) Demonstration of the phosphorylation-dependent interaction of tryptophan hydroxylase with the 14-3-3 protein. Biochem. Biophys. Res. Commun. 194, 144-149.
14. Garber S. L. and Makman M. H. (1987) Regulation of tryptophan hydroxylase activity by a cyclic AMP-dependent mechanism in rat striatum. Mol. Brain Res. 3, 1-10.
15. Gartside S. E., Cowen P. J., and Sharp T. (1992) Evidence that the large neutral amino acid L-valine decreases electrically evoked release of 5-HT in rat hippocampus in vivo. Psychopharmacology 109, 251-253.
16. Gibbs B. S., Wojchowski D., and Benkovic S. J. (1993) Expression of rat liver phenylalanine hydroxylase in insect cells and site-directed mutagenesis of putative non-heme iron-binding sites. J. Biol. Chem. 11, 8046-8052.
17. Golden R. N. (1990) Serotonin in psychiatric disorders. Psychiatr. Ann. 20, 556-602.
18. Gorman C. M., Merlino G. T., Willingham M. C., Pastan I., and Howard B. H. (1982) The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79, 6777-6781.
19. Grennet H. E., Ledley F. D., Reed L. L., and Woo S. L. (1987) Functional domains and evolution of aromatic amino acid hydroxylases. Proc. Natl. Acad. Sci. USA 84, 5530-5534.
20. Guan K.-L. and Dixon J. E. (1991) Eukaryotic proteins expressed in Escherichia coli: an improved thrombin cleavage and purification procedure of fusion proteins with glutathione S-transferase. Anal. Biochem. 192, 262-267.
21. Haavik J., Le Bourdelles B., Martinez A., Flatmark T., and Mallet J. (1991) Recombinant human tyrosine hydroxylase isozymes: reconstitution with iron and inhibitory effect of other metal ions. Eur. J. Biochem. 199, 371-378.
22. Hamon M., Bourgoin S., Hery F., and Simonnet G. (1978) Activation of tryptophan hydroxylase by adenosine triphosphate, magnesium, and calcium. Mol. Pharmacol. 14, 99-110.
23. Ichimura T., Uchiyama J., Kunihiro O., Ito M., Horigome T., Omata S., Shinkai F., Kaji H., and Isobe T. (1995) Identification of the site of interaction of the 14-3-3 protein with phosphorylated tryptophan hydroxylase. J. Biol. Chem. 270, 28515-28518.
24. Iwaki M., Phillips R. S., and Kaufman S. (1986) Proteolytic modification of the amino-terminal and carboxyl-terminal regions of rat hepatic phenylalanine hydroxylase. J. Biol. Chem. 261, 2051-2056.
25. Jequier E., Lovenberg W., and Sjoerdsma A. (1967) Tryptophan hydroxylase inhibition: the mechanism by which p-chlorophenylalanine depletes brain serotonin. Mol. Pharmacol. 3, 274-278.
26. Johansen P. A., Wolf W. A., and Kuhn D. M. (1991) Inhibition of tryptophan hydroxylase by benserazide and other catechols. Biochem. Pharmacol. 41, 625-628.
27. Johansen P. A., Jennings I., Cotton R. G. H., and Kuhn D. M. (1995) Tryptophan hydroxylase is phosphorylated by protein kinase A. J. Neurochem. 65, 882-888.
28. Johansen P. A., Jennings I., Cotton R. G. H., and Kuhn D. M. (1996) Phosphorylation and activation of tryptophan hydroxylase by endogenous protein kinase A. J. Neurochem. 66, 817-823.
29. Kaufman S. (1993) The phenylalanine hydroxylating system. Adv. Enzy. Related Areas Mol. Biol. 67, 77-264.
30. Kawasaki E. S. and Wang A. M. (1989) Detection of gene expression, in PCR Technology: Principles and Applications for DNA Amplification (Erlich H. A., ed), pp. 89-97. Stockton Press, New York.
31. Kuhn D. M., Meyer M. A., and Lovenberg W. (1979a) Comparisons of tryptophan hydroxylase from a malignant murine mast cell tumor and rat mesencephalic tegmentum. Arch. Biochem. Biophys. 199, 355-361.
32. Kuhn D. M., Rosenberg R. C., and Lovenberg W. (1979b) Determination of some molecular parameters of tryptophan hydroxylase from rat brainstem and murine mast cell. J. Neurochem. 33, 15-21.
33. 2+: dependence on calmodulin. Proc. Natl. Acad. Sci. USA 77, 4688-4691.
34. Kuhn D. M., Ruskin B., and Lovenberg W. (1980b) Tryptophan hydroxylase: the role of oxygen, iron, and sulfhydryl groups as determinants of stability and catalytic activity. J. Biol. Chem. 255, 4137-4143.
35. Kuhn D. M., Wolf W., and Lovenberg W. (1980c) Review of the role of the central serotonergic neuronal system in blood pressure regulation. Hypertension 2, 243-255.
36. Laemmli U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-684.
37. Lehnert H. and Wurtman R. J. (1993) Amino acid control of neurotransmitter synthesis and release: physiological and clinical implications. Psychother. Psychosom. 60, 18-32.
38. Lohse D. L. and Fitzpatrick P. F. (1993) Identification of the intersubunit binding region in rat tyrosine hydroxylase. Biochem. Biophys. Res. Commun. 197, 1543-1548.
39. Nakata H. and Fujisawa H. (1981) Simple and rapid purification of tryptophan 5-monooxygenase from rabbit brain by affinity chromatography. J. Biochem. 90, 567-569.
40. Nakata H. and Fujisawa H. (1982) Purification and properties of tryptophan 5-monooxygenase from rat brain stem. Eur. J. Biochem. 122, 41-47.
41. Oluyomi A. O., Gibson E. L., Barnfield A. M. C., and Curzon G. (1994) D-Fenfluramine and D-norfenfluramine hypophagias do not require increased hypothalamic 5-hydroxytryptamine release. Eur. J. Pharmacol. 264, 111-115.
42. Park D. H., Stone D. M., Kim K. S., and Job. T. H. (1994) Characterization of recombinant mouse tryptophan hydroxylase expressed in Escherichia coli. Mol. Cell. Neurosci. 5, 87-93.
43. Paul S. M. (1990) Serotonin and its effects on human behavior. J. Clin. Psychiatry 51, 1-70.
44. Ribiero P., Wang Y., Citron B. A., and Kaufman S. (1993) Deletion mutagenesis of rat PC12 tyrosine hydroxylase regulatory and catalytic domains. J. Mol. Neurosci. 2, 125-139.
45. Shiman R., Gray D. W., and Hill M. A. (1994) Regulation of rat liver phenylalanine hydroxylase. I. Kinetic properties of the enzyme's iron and enzyme reduction site. J. Biol. Chem. 269, 24637-24646.
46. Smith D. B. and Johnson K. S. (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67, 31-40.
47. States J, C., Gebauer B. K., and Berberoglu E. D. (1990) Rapid polymerase chain reaction-based analysis of DNA fragments cloned in LacZ vectors. Technique 2, 246-253.
48. Tipper J. P., Citron B. A., Ribeiro P., and Kaufman S. (1994) Cloning and expression of rabbit and human brain tryptophan hydroxylase cDNA in Escherichia coli. Arch. Biochem. Biophys. 315, 445-453.
49. Vrana K. E., Rucker P. I., and Kumer S. C. (1994a) Recombinant rabbit tryptophan hydroxylase is a substrate for cAMP-dependent protein kinase. Life Sci. 55, 1045-1052.
50. Vrana K. E., Walker S. J., Rucker P., and Lui X. (1994b) A carboxyl-terminal leucine zipper is required for tyrosine hydroxylase tetramer formation. J. Neurochem. 63, 2014-2020.
51. Whitaker-Azmitia P. M. and Peroutka S. J., eds (1990) The Neuropharmacology of Serotonin (Ann. NY Acad. Sci. 600). New York Academy of Sciences, New York.
52. Wu J., Filer D., Friedhoff A. J., and Goldstein M. (1992) Site-directed mutagenesis of tyrosine hydroxylase: role of serine 40 in catalysis. J. Biol. Chem. 267, 25754-25758.
53. Yamauchi T. and Fujisawa H. (1979) Regulation of rat brainstem tryplophan 5-monooxygenase: calcium-dependent reversible activation by ATP and magnesium. Arch. Biochem. Biaphys. 198, 219-226.
54. Yang X.-J. and Kaufman S. (1994) High-level expression and deletion mutagenesis of human tryptophan hydroxylase. Proc. Natl. Acad. Sci. USA 91, 6659-6663.