Tryptophan hydroxylase: Cloning and expression of the rat brain enzyme in mammalian cells

Journal of Neurochemistry

Volumn 67, Issue 3, 1996, Pages 900-906

  D'Sa, Carrol M ^a   Arthur Jr , Robert E ^a   States, J Christopher ^b   Kuhn, Donald M ^a,b,c  

^a Cell and Clin Neurobiology Program   (United States)

^b WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Metropol Center for High Technology   (United States)

Author keywords

Brain; Cloning expression; Recombinant enzyme; Tryptophan hydroxylase

Indexed keywords

CATALASE; COMPLEMENTARY DNA; DEFEROXAMINE; DOPAMINE; HEPARIN; MESSENGER RNA; MONOCLONAL ANTIBODY; PHENYLALANINE 4 MONOOXYGENASE; PHOSPHATIDYLSERINE; RECOMBINANT PROTEIN; TYROSINE 3 MONOOXYGENASE;

ANIMAL CELL; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; FIBROBLAST CULTURE; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; MAMMAL CELL; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY; RAT; SEROTONINERGIC SYSTEM;

ANIMALIA; MAMMALIA;

EID: 0029848477     PISSN: 00223042     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1471-4159.1996.67030900.x     Document Type: Article

References (34)

1. Boularand S., Darmon M. C., Ganem Y., Launay J. M., and Mallet J. (1990) Complete coding sequence of human tryptophan hydroxylase. Nucleic Acids Res. 18, 4257.
2. Bradford M. M. (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Cotton R. G. H., McAdam W., Jennings I., and Morgan F. J. (1988) A monoclonal antibody to aromatic amino acid hydroxylases: identification of the epitope. Biochem. J. 255, 193-196.
4. Darmon M. C., Guibert B., Leviel V., Ehret M., Maitre M., and Mallet J. (1988) Sequence of two mRNAs encoding active rat tryptophan hydroxylase. J. Neurochem. 51, 312-316.
5. Dix T. A., Kuhn D. M., and Benkovic S. J. (1987) Mechanism of oxygen activation by tyrosine hydroxylase. Biochemistry 26, 3354-3361.
6. Dumas S., Darmon M. C., Delort J., and Mallet J. (1989) Differential control of tryptophan hydroxylase expression in raphe and in pineal gland: evidence for a role in translation efficiency. J. Neurosci. Res. 24, 537-547.
7. Ehret M., Gobaille S., Cash C. D., Mandel P., and Maitre M. (1987) Regional distribution in rat brain of tryptophan hydroxylase apoenzyme determined by enzyme-linked immunoassay. Neurosci. Lett. 73, 71-76.
8. Golden R. N. (1990) Serotonin in psychiatric disorders. Psychiatr. Ann. 20, 556-602.
9. Gorman C. M., Merlino G. T., Willingham M. C., Pastan I., and Howard B. H. (1982) The Rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79, 6777-6781.
10. Grennet H. E., Ledley F. D., Reed L. L., and Woo S. L. (1987) Functional domains and evolution of aromatic amino acid hydroxylases. Proc. Natl. Acad. Sci. USA 84, 5530-5534.
11. Haavik J., Le Bourdelles B., Martinez A., Flatmark T., and Mallet J. (1991) Recombinant human tyrosine hydroxylase isozymes: reconstitution with iron and inhibitory effect of other metal ions. Eur. J. Biochem. 199, 371-378.
12. Harlow E. and Lane D. (1988) Immunoblotting, in Antibodies - A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
13. Jequier E., Lovenberg W., and Sjoerdsma A. (1967) Tryptophan hydroxylase inhibition: the mechanism by which p -chlorophenylalanine depletes brain serotonin. Mol. Pharmacol. 3, 274-278.
14. Johansen P. A., Wolf W. A., and Kuhn D. M. (1991) Inhibition of tryptophan hydroxylase by benserazide and other catechols. Biochem. Pharmacol. 41, 625-628.
15. Johansen P. A., Jennings I., Cotton R. G. H., and Kuhn D. M. (1992) Immobilization of tryptophan hydroxylase by immune adsorption: a method to study regulation of catalytic activity. Brain Res. Bull. 29, 949-953.
16. Johansen P. A., Jennings I., Cotton R. G. H., and Kuhn D. M. (1995) Tryptophan hydroxylase is phosphorylated by protein kinase A. J. Neurochem. 65, 882-888.
17. Johansen P. A., Jennings I., Cotton R. G. H., and Kuhn D. M. (1996) Phosphorylation and activation of tryptophan hydroxylase by exogenous protein kinase A. J. Neurochem. 66, 817-823.
18. Kawasaki E. S. and Wang A. M. (1989) Detection of gene expression, in PCR Technology: Principles and Applications for DNA Amplification (Erlich H. A., ed), pp. 89-97. Stockton Press, New York.
19. Kim K. S., Wessel T., Stone D. M., Carver C. H., Job T. H., and Park D. H. (1991) Molecular cloning and characterization of DNA encoding tryptophan hydroxylase from rat central serotonergic neurons. Mol. Brain Res. 9, 277-283.
20. Kuhn D. M. and Billingsley M. L. (1987) Tyrosine hydroxylase: purification from PC-12 cells, characterization, and production of antibodies. Neurochem. Int. 11, 463-475.
21. Kuhn D. M. and Lovenberg W. (1986) Tryptophan hydroxylase, in Chemistry and Biochemistry of Pterins (Blakely R. L. and Benkovic S., eds), pp. 353-382. Wiley, New York.
22. Kuhn D. M., Meyer M. A., and Lovenberg W. (1979a) Comparisons of tryptophan hydroxylase from a malignant murine mast cell tumor and rat mesencephalic tegmentum. Biochem. Pharmacol. 28, 3255-3260.
23. Kuhn D. M., Rosenberg R., and Lovenberg W. (1979b) Determination of some molecular parameters of tryptophan hydroxylase from rat brainstem and murine mast cell. J. Neurochem. 33, 15-21.
24. Kuhn D. M., Ruskin B., and Lovenberg W. (1980) Tryptophan hydroxylase: the role of oxygen, iron, and sulfhydryl groups as determinants of stability and catalytic activity. J. Biol. Chem. 255, 4137-4143.
25. Park D. H., Stone D. M., Kim K. S., and Joh T. H. (1994) Characterization of recombinant mouse tryptophan hydroxylase expressed in Escherichia coli. Mol. Cell. Neurosci. 5, 87-93.
26. Paul S. M. (1990) Serotonin and its effects on human behavior. J. Clin. Psychiatry 51 (Suppl.), 1-70.
27. Shiman R., Gray D. W., and Hill M. A. (1994) Regulation of rat liver phenylalanine hydroxylase. I. Kinetic properties of the enzyme's iron and enzyme reduction site. J. Biol. Chem. 269, 24637-24646.
28. Smith M. H. (1970) Molecular weights of proteins and some other materials including sedimentation, diffusion and frictional coefficients and partial specific volumes, in Handbook of Biochemistry (Sober H. A., ed), pp. 10-25. The Chemical Rubber Co., Cleveland.
29. States J. C., Gebauer B. K., and Berberoglu E. D. (1990) Rapid polymerase chain reaction based analysis of DNA fragments cloned in LacZ vectors. Technique J. Methods Cell Mol. Biol. 2, 246-253.
30. Tipper J. P., Citron B. A., Ribeiro P., and Kaufman S. (1994) Cloning and expression of rabbit and human brain tryptophan hydroxylase DNA in Escherichia coli. Arch. Biochem. Biophys. 315, 445-453.
31. Tong J. H. and Kaufman S. (1975) Tryptophan hydroxylase: purification and some properties of the enzyme from rabbit hindbrain. J. Biol. Chem. 250, 4152-4158.
32. Vrana K. E., Raker P. J., and Kumer S. C. (1994) Recombinant rabbit tryptophan hydroxylase is a substrate for AMP-dependent protein kinase. Life Sci. 55, 1045-1052.
33. Whitaker-Azmitia P. M. and Peroutka S. J., eds (1990) The neuropharmacology of serotonin. Ann. NY Acad. Sci. 600, 1-718.
34. Yang X.-J. and Kaufman S. (1994) High-level expression and deletion mutagenesis of human tryptophan hydroxylase. Proc. Natl. Acad. Sci. USA 91, 6659-6663.