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For leading references see: (a) Abbott, S. D,; Lane-Bell, P.; Sidhu, K. P. S.; Vederas, J. C. J. Am. Chem. Soc. 1994, 116, 6513-6520. (b) Song, Y. H.; Niederer. D.; Lane-Bell, P.; Lam, L. K. P.; Crawley, S.; Palcic, M. M.; Pickard, M. A.; Pruess, D. L.; Vederas, J. C. J. Org. Chem. 1994, 59, 5784-5793. (c) Williams, R. M.; Yuan, C. J. Org. Chem. 1992, 57, 6519-6527. (d) Bold, G.; Allmendinger, T.; Herold, P.; Moesch, L.; Schär, H.-P.; Duthaler, R. O. Helv. Chim. Acta 1992, 75, 865-882. (e) Jurgens, A. R. Tetrahedron Lett. 1992, 33, 4727-4730. (f) Mengin-Lecreulx, D.; Blanot, D.; Van Heijenoort, J. J. Bacteriol. 1994, 176, 4321-4327. (g) Williams, R. M.; Fegley, G. J.; Gallegos, R.; Schaefer, F.; Pruess, D. L. Tetrahedron 1996, 52, 1149-1164.
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For leading references see: (a) Abbott, S. D,; Lane-Bell, P.; Sidhu, K. P. S.; Vederas, J. C. J. Am. Chem. Soc. 1994, 116, 6513-6520. (b) Song, Y. H.; Niederer. D.; Lane-Bell, P.; Lam, L. K. P.; Crawley, S.; Palcic, M. M.; Pickard, M. A.; Pruess, D. L.; Vederas, J. C. J. Org. Chem. 1994, 59, 5784-5793. (c) Williams, R. M.; Yuan, C. J. Org. Chem. 1992, 57, 6519-6527. (d) Bold, G.; Allmendinger, T.; Herold, P.; Moesch, L.; Schär, H.-P.; Duthaler, R. O. Helv. Chim. Acta 1992, 75, 865-882. (e) Jurgens, A. R. Tetrahedron Lett. 1992, 33, 4727-4730. (f) Mengin-Lecreulx, D.; Blanot, D.; Van Heijenoort, J. J. Bacteriol. 1994, 176, 4321-4327. (g) Williams, R. M.; Fegley, G. J.; Gallegos, R.; Schaefer, F.; Pruess, D. L. Tetrahedron 1996, 52, 1149-1164.
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For leading references see: (a) Abbott, S. D,; Lane-Bell, P.; Sidhu, K. P. S.; Vederas, J. C. J. Am. Chem. Soc. 1994, 116, 6513-6520. (b) Song, Y. H.; Niederer. D.; Lane-Bell, P.; Lam, L. K. P.; Crawley, S.; Palcic, M. M.; Pickard, M. A.; Pruess, D. L.; Vederas, J. C. J. Org. Chem. 1994, 59, 5784-5793. (c) Williams, R. M.; Yuan, C. J. Org. Chem. 1992, 57, 6519-6527. (d) Bold, G.; Allmendinger, T.; Herold, P.; Moesch, L.; Schär, H.-P.; Duthaler, R. O. Helv. Chim. Acta 1992, 75, 865-882. (e) Jurgens, A. R. Tetrahedron Lett. 1992, 33, 4727-4730. (f) Mengin-Lecreulx, D.; Blanot, D.; Van Heijenoort, J. J. Bacteriol. 1994, 176, 4321-4327. (g) Williams, R. M.; Fegley, G. J.; Gallegos, R.; Schaefer, F.; Pruess, D. L. Tetrahedron 1996, 52, 1149-1164.
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For leading references see: (a) Abbott, S. D,; Lane-Bell, P.; Sidhu, K. P. S.; Vederas, J. C. J. Am. Chem. Soc. 1994, 116, 6513-6520. (b) Song, Y. H.; Niederer. D.; Lane-Bell, P.; Lam, L. K. P.; Crawley, S.; Palcic, M. M.; Pickard, M. A.; Pruess, D. L.; Vederas, J. C. J. Org. Chem. 1994, 59, 5784-5793. (c) Williams, R. M.; Yuan, C. J. Org. Chem. 1992, 57, 6519-6527. (d) Bold, G.; Allmendinger, T.; Herold, P.; Moesch, L.; Schär, H.-P.; Duthaler, R. O. Helv. Chim. Acta 1992, 75, 865-882. (e) Jurgens, A. R. Tetrahedron Lett. 1992, 33, 4727-4730. (f) Mengin-Lecreulx, D.; Blanot, D.; Van Heijenoort, J. J. Bacteriol. 1994, 176, 4321-4327. (g) Williams, R. M.; Fegley, G. J.; Gallegos, R.; Schaefer, F.; Pruess, D. L. Tetrahedron 1996, 52, 1149-1164.
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Jurgens, A.R.1
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For leading references see: (a) Abbott, S. D,; Lane-Bell, P.; Sidhu, K. P. S.; Vederas, J. C. J. Am. Chem. Soc. 1994, 116, 6513-6520. (b) Song, Y. H.; Niederer. D.; Lane-Bell, P.; Lam, L. K. P.; Crawley, S.; Palcic, M. M.; Pickard, M. A.; Pruess, D. L.; Vederas, J. C. J. Org. Chem. 1994, 59, 5784-5793. (c) Williams, R. M.; Yuan, C. J. Org. Chem. 1992, 57, 6519-6527. (d) Bold, G.; Allmendinger, T.; Herold, P.; Moesch, L.; Schär, H.-P.; Duthaler, R. O. Helv. Chim. Acta 1992, 75, 865-882. (e) Jurgens, A. R. Tetrahedron Lett. 1992, 33, 4727-4730. (f) Mengin-Lecreulx, D.; Blanot, D.; Van Heijenoort, J. J. Bacteriol. 1994, 176, 4321-4327. (g) Williams, R. M.; Fegley, G. J.; Gallegos, R.; Schaefer, F.; Pruess, D. L. Tetrahedron 1996, 52, 1149-1164.
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For leading references see: (a) Abbott, S. D,; Lane-Bell, P.; Sidhu, K. P. S.; Vederas, J. C. J. Am. Chem. Soc. 1994, 116, 6513-6520. (b) Song, Y. H.; Niederer. D.; Lane-Bell, P.; Lam, L. K. P.; Crawley, S.; Palcic, M. M.; Pickard, M. A.; Pruess, D. L.; Vederas, J. C. J. Org. Chem. 1994, 59, 5784-5793. (c) Williams, R. M.; Yuan, C. J. Org. Chem. 1992, 57, 6519-6527. (d) Bold, G.; Allmendinger, T.; Herold, P.; Moesch, L.; Schär, H.-P.; Duthaler, R. O. Helv. Chim. Acta 1992, 75, 865-882. (e) Jurgens, A. R. Tetrahedron Lett. 1992, 33, 4727-4730. (f) Mengin-Lecreulx, D.; Blanot, D.; Van Heijenoort, J. J. Bacteriol. 1994, 176, 4321-4327. (g) Williams, R. M.; Fegley, G. J.; Gallegos, R.; Schaefer, F.; Pruess, D. L. Tetrahedron 1996, 52, 1149-1164.
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m = 5.20 mM (Tris, pH 8.0, 30 °C).
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9444258684
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note
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The procedure is a modification of that initially developed by Gilvarg and Peterkofsky: see refs 7 and 9b.
-
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54
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3042918663
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Literature values for other DAP pathway enzyme purifications: (a) L-THDPA succinyltransferase 1900-fold from E. coli; see ref 8. (b) DAP desuccinylase (EC 3.5.1.18) 7100-fold from E. coli; see ref 10b. (c) DAP epimerase (EC 5.1.1.7) 6000-fold from E. coli; see ref 11. (d) DAP dehydrogenase (EC 1.4.1.16) 290-fold from B. sphaericus, see ref 13b. (d) DAP decarboxylase (EC4.1.1.20) 500-fold from B. sphaericus: Asada, Y.; Tanizawa, K.; Kawabata, Y.; Misono, H.; Soda, K. Agric. Biol. Chem. 1981, 45, 1513-1514. Andersen, Å. B.; Hansen, E. B. Gene 1993, 124, 105-109.
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note
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10a demonstrate that acetylated DAP analogues are not substrates for this hydrolytic enzyme.
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Shareware: Kevin Raner, 77 Therese Ave, Mt. Waverly 3149, Australia.
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