Poliovirus/hepatitis C virus (internal ribosomal entry site-core) chimeric viruses: Improved growth properties through modification of a proteolytic cleavage site and requirement for core RNA sequences but not for core-related polypeptides

Journal of Virology

Volumn 73, Issue 2, 1999, Pages 1546-1554

  Zhao, Wei Dong ^a   Wimmer, Eckard ^a   Lahser, Frederick C ^a,b  

^a STONY BROOK UNIVERSITY   (United States)

^b MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYPEPTIDE; PROTEINASE; SIGNAL PEPTIDASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; FRAMESHIFT MUTATION; GENETIC ANALYSIS; HEPATITIS C VIRUS; HUMAN; HUMAN CELL; IMMUNOBLOTTING; OPEN READING FRAME; PHENOTYPE; POLIOMYELITIS VIRUS; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; RIBOSOME; RNA SEQUENCE; RNA TRANSCRIPTION; VIRUS CAPSID; VIRUS REPLICATION; VIRUS TITRATION; VIRUS TRANSCRIPTION;

EID: 0005042486     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.73.2.1546-1554.1999     Document Type: Article

References (47)

1. Alexander, L., H. H. Lu, and E. Wimmer. 1994. Polioviruses containing picornavirus type 1 and/or type 2 internal ribosomal entry site elements: genetic hybrids, and the expression of a foreign gene. Proc. Natl. Acad. Sci. USA 91:1406-1410.
2. Alter, M. J. 1996. Epidemiology of hepatitis C in the West. Semin. Liver Dis. 15:5-14.
3. Andino, R., G. E. Kieckhof, P. L. Achacoso, and D. Baltimore. 1993. Poliovirus RNA synthesis utilizes an RNP complex formed around the 5′-end of viral RNA. EMBO J. 9:3587-3598.
4. Andino, R., D. Silvera, S. D. Suggett, P. L. Achacoso, C. J. Miller, D. Baltimore, and M. B. Feinberg. 1994. Engineering poliovirus as a vaccine vector for the expression of diverse antigens. Science 265:1448-1451.
5. Brown, E. A., H. Zhang, L.-H. Ping, and S. M. Lemon. 1992. Secondary structure of the 5′ nontranslated regions of hepatitis C virus and pestivirus genomic RNAs. Nucleic Acids Res. 20:5041-5045.
6. Bruix, J., J. M. Barrera, X. Calvet, G. Ercilla, J. Costa, J. M. Sanchez-Tapias, M. Ventura, M. Vall, M. Bruguera, C. Bru, et al. 1989. Prevalence of antibodies to hepatitis C virus in Spanish patients with hepatocellular carcinoma and hepatic cirrhosis. Lancet 2:1004-1006.
7. Choo, Q.-L., K. H. Richman, J. H. Han, K. Berger, C. Lee, C. Dong, C. Gallegos, D. Colt, A. Medina-Selby, P. J. Barr, A. J. Weiner, D. W. Bradley, G. Kuo, and M. Houghton. 1991. Genetic organization and diversity of the hepatitis C virus. Proc. Natl. Acad. Sci. USA 88:2451-2455.
8. Chow, M., and N. Moscufo. 1995. Myristoyl modification of viral proteins: assays to assess functional roles. Methods Enzymol. 250:495-509.
9. Chow, M., J, F. E. Newman, D. Filman, J. M. Hogle, D. J. Rowlands, and F. Brown. 1987. Myristylation of picornavirus capsid protein VP4 and its structural significance. Nature 327:482-486.
10. Di Bisceglie, A. M., Z. D. Goodman, K. G. Ishak, J. H. Hoofnagle, J. J. Melpolder, and H. J. Alter. 1991. Long-term clinical and histopathological follow-up of chronic posttransfusion hepatitis. Hepatology 14:969-974.
11. Gromeier, M., L. Alexander, and E. Wimmer. 1996. Internal ribosomal entry site substitution eliminates neurovirulence in intergeneric poliovirus recombinants. Proc. Natl. Acad. Sci. USA 93:2370-2375.
12. Harris, K. S., C. U. T. Hellen, and E. Wimmer. 1990. Proteolytic processing in the replication of picornaviruses. Semin. Virol. 1:323-333.
13. Hellen, C. U. T., M. Fäcke, H.-G. Krāsslich, C.-K. Lee, and E. Wimmer. 1992. Characterization of poliovirus 2A proteinase by mutational analysis: residues required for autocatalytic activity are essential for induction of cleavage of eukaryotic initiation factor 4F polypeptide p220. J. Virol. 65: 4226-4231.
14. Ho, S. N., H. D. Hunt, R. M. Horton, J. K. Pullen, and L. R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
15. Honda, M., E. A. Brown, and S. M. Lemon. 1996. Stability of a stem-loop involving the initiator AUG controls the efficiency of internal initiation of translation on hepatitis C virus RNA. RNA 2:955-968.
16. Jang, S. K., M. V. Davies, R. J. Kaufman, and E. Wimmer. 1989. Initiation of protein synthesis by internal entry of ribosomes into the 5′ nontranslated region of encephalomyocarditis virus RNA in vitro. J. Virol. 63:1651-1660.
17. Jang, S. K., H.-G. Kräusslich, M. J. H. Nicklin, G. M. Duke, A. C. Palmenberg, and E. Wimmer. 1988. A segment of the 5′ nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation. J. Virol. 62:2636-2643.
18. Kolykhalov, A. A., E. V. Agapov, K. J. Blight, K. Mihalik, S. M. Feinstone, and C. M. Rice. 1997. Transmission of hepatitis C by intrahepatic inoculation with transcribed RNA. Science 277:570-574.
19. Kozak, M. J. 1989. The scanning model for translation: an update. J. Cell Biol. 108:229-241.
20. Kräusslich, H.-G., and E. Wimmer. 1988. Viral proleinases. Annu. Rev. Biochem. 57:701-754.
21. Kuo, G., Q. L. Choo, H. J. Alter, G. L. Gitnick, A. G. Redeker, R. H. Purcell, T. Miyamura, L. Dienstag, M. J. Alter, C. E. Stevens, G. E. Tegtmeier, F. Bonino, M. Colombo, W. S. Lee, C. Kuo, K. Berger, J. R. Shuster, L. R. Overby, D. W. Bradely, and M. Houghton. 1989. An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis. Science 244:362-364.
22. Lahser, F. 1996. Unpublished data.
23. Lee, C. K., and E. Wimmer. 1988. Proteolytic processing of poliovirus polyproteins: elimination of 2A pro-mediated, alternative cleavage of polypeptide 3CD by in vitro mutagenesis. Virology 166:405-114.
24. Lu, H. H., L. Alexander, and E. Wimmer. 1995. Construction and genetic analysis of dicistronic polioviruses containing open reading frames for epitopes of human immunodeficiency virus type 1 gp120. J. Virol. 69:4797-4806.
25. Lu, H. H., and E. Wimmer. 1996. Poliovirus chimeras replicating under the translational control of genetic elements of hepatitis C virus reveal unusual properties of the internal ribosomal entry site of hepatitis C virus. Proc. Natl. Acad. Sci. USA 93:1412-1417.
26. pro. J. Virol. 68:7507-7515.
27. Matsuura, Y., and T. Miyamura. 1993. The molecular biology of hepatitis C virus. Semin. Virol. 4:297-304.
28. Miller, R. H., and R. T. Percell. 1990. Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proc. Natl. Acad. Sci. USA 87:2057-2061.
29. Molla, A., S. K. Jang, A. V. Paul, Q. Reuer, and E. Wimmer. 1992. Cardioviral internal ribosomal entry site is functional in a genetically engineered dicistronic poliovirus. Nature 356:255-257.
30. Molla, A., A. V. Paul, and E. Wimmer. 1991. Cell-free, de novo synthesis of poliovirus. Science 254:1647-1651.
31. Moradpour, D., T. Wakita, K. Tokushige, R. I. Carlson, K. Krawczynski, and J. R. Wands. 1996. Characterization of three novel monoclonal antibodies against hepatitis C virus core protein. J. Med. Virol. 48:234-241.
32. Mueller, S., and E. Wimmer. 1998. Expression of foreign proteins by poliovirus polyprotein fusion: analysis of genetic stability reveals rapid deletions and formation of cardioviruslike open reading frames. J. Virol. 72:20-31.
33. Okamoto, H., K. Kurai, S.-I. Okada, K. Yamamoto, H. Lizuka, T. Tanaka, S. Fukuda, F. Tsuda, and S. Mishiro. 1992. Full-length sequence of a hepatitis C virus genome having poor homology to reported isolates: comparative study of four distinct genotypes. Virology 188:331-341.
34. Paul, A. V., A. Schultz, S. E. Pincus, S. Oroszlan, and E. Wimmer. 1987 Capsid protein VP4 of poliovirus is N-myristoylated. Proc. Natl. Acad. Sci. USA 84:7827-7831.
35. Pelletier, J., and N. Sonenberg. 1989. Internal binding of eukaryotic ribosomes on poliovirus RNA: translation in HeLa cell extracts. J. Virol. 63:441-444.
36. Pelletier, J., and N. Sonenberg. 1988. Internal initiation of translation of eukaryotic mRNA directed by a sequence derived from poliovirus RNA. Nature 334:320-325.
37. Reynolds, J. E., A. Kaminski, A. R. Carroll, B. E. Clarke, D. J. Rowlands, and R. J. Jackson. 1996. Internal initiation of translation of hepatitis C virus RNA: the ribosome entry site is at the authentic initiation codon. RNA 2:867-878.
38. Reynolds, J. E., A. Kaminski, H. J. Kettinen, K. Grace, B. E. Clarke, A. R. Carroll, D. J. Rowlands, and R. J. Jackson. 1995. Unique features of internal initiation of hepatitis C virus RNA translation. EMBO J. 14:6010-6020.
39. Rijnbrand, R., P. Bredenbeek, T. van der Straaten, L. Whetter, G. Inchauspe, S. Lemon, and W. Spaan. 1995. Almost the entire 5′ non-translated region of hepatitis C virus is required for cap-independent translation. FEBS Lett. 365:115-119.
40. Saito, I., T. Miyamura, A. Ohbayashi, H. Harada, T. Katayama, S. Kikuchi, Y. Watanabe, S. Koi, M. Onji, Y. Ohta, et al. 1990. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 87:6547-6549.
41. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory. Cold Spring Harbor, N. Y.
42. Santolini, E., G. Migliaccio, and N. La Monica. 1994. Biosynthesis and biochemical properties of the hepatitis C virus core protein. J. Virol. 68: 3631-3641.
43. Tsukiyama-Kohara, K., N. Lizuka, M. Kohara, and A. Momoto. 1992. Internal ribosome entry site within hepatitis C virus RNA. J. Virol. 66:1476-1483.
44. van der Werf, S., J. Bradley, E. Wimmer, F. W. Studier, and J. J. Dunn. 1986. Synthesis of infectious poliovirus RNA by purified T7 RNA polymerase. Proc. Natl. Acad. Sci. USA 78:2330-2334.
45. Wang, C., P. Sarnow, and A. Siddiqui. 1993. Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism. J. Virol. 67:3338-3344.
46. Wimmer, E., C. U. T. Hellen, and X. M. Cao. 1993. Genetics of poliovirus. Annu. Rev. Genet. 27:353-436.
47. Zhao, W. D., and F. C. Lahser. 1997. Unpublished data.