Deamidations in recombinant human phenylalanine hydroxylase: Identification of labile asparagine residues and functional characterization of Asn → Asp mutant forms

Journal of Biological Chemistry

Volumn 278, Issue 17, 2003, Pages 15142-15152

  Carvalho, Raquel Negrão ^a   Solstad, Therese ^a   Bjørgo, Elisa ^b   Barroso, João Filipe ^a   Flatmark, Torgeir ^a  

^a UNIVERSITY OF BERGEN   (Norway)

^b UNIVERSITY OF OSLO   (Norway)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOCATALYSTS; ELECTROPHORESIS; ENZYME INHIBITION; ENZYMES; SUBSTRATES;

MUTAGENESIS;

BIOCHEMISTRY;

ASPARAGINE; ASPARTIC ACID; CYCLIC AMP DEPENDENT PROTEIN KINASE; GLYCINE; PHENYLALANINE; PHENYLALANINE 4 MONOOXYGENASE; RECOMBINANT ENZYME; SERINE; TETRAHYDROBIOPTERIN;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; DEAMINATION; ELECTROPHORESIS; ENZYME MECHANISM; ENZYME PHOSPHORYLATION; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE MUTATION; MASS SPECTROMETRY; PHENOTYPE; PHENYLKETONURIA; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; WILD TYPE;

AMIDES; AMINO ACID SEQUENCE; ASPARAGINE; ASPARTIC ACID; CATALYTIC DOMAIN; ENZYME STABILITY; HUMANS; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; PHENYLALANINE HYDROXYLASE; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ESCHERICHIA COLI;

EID: 0003285188     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M212180200     Document Type: Article

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