Enzymatic properties of mouse 25-hydroxyvitamin D3 1α-hydroxylase expressed in Escherichia coli

European Journal of Biochemistry

Volumn 259, Issue 3, 1999, Pages 731-738

  Sakaki, Toshiyuki ^b   Sawada, Natsumi ^b   Takeyama, Ken Ichi ^b   Kato, Shigeaki ^b   Inouye, Kuniyo ^a  

^a KYOTO UNIVERSITY   (Japan)

^b NONE

Author keywords

1 hydroxylase; CYP27B1; E. coli; P450; Vitamin D

Indexed keywords

CALCIDIOL 1 MONOOXYGENASE; CALCIDIOL 1 MONOOXYGENASE INHIBITOR; CARBON MONOXIDE; COLECALCIFEROL; KETOCONAZOLE; METYRAPONE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING KINETICS; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SUBUNIT; ESCHERICHIA COLI; HYDROXYLATION; MOLECULAR CLONING; MOUSE; NONHUMAN; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; VITAMIN D METABOLISM;

24,25-DIHYDROXYVITAMIN D 3; 25-HYDROXYVITAMIN D3 1-ALPHA-HYDROXYLASE; ANIMALS; CALCIFEDIOL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENZYME INHIBITORS; ESCHERICHIA COLI; GENE EXPRESSION; HYDROXYLATION; KIDNEY; KINETICS; MASS SPECTROMETRY; MICE; MOLECULAR STRUCTURE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0002819595     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00096.x     Document Type: Article

References (32)

1. 3 in the intestine. Science 173, 51-54.
2. 3 metabolically active in the intestines. Proc. Natl Acad. Sci. USA 68, 803-804.
3. 3. Boyle, I.T., Gray, R.W. & DeLuca, H.F. (1971) Regulation by calcium of in vivo synthesis of 1,25-dihydroxycholecalciferol and 21,25-dihydroxycholecalciferol. Proc. Natl Acad. Sci. USA 68, 2131-2134.
4. 4. Lawson, D.E.M., Fraser, D.R., Kodicek, E., Morris, H.R. & Williams, D.H. (1971) Identification of 1,25-dihydroxycholecalciferol, a new kidney hormone controlling calcium metabolism. Nature 230, 228-230.
5. 3-1α-hydroxylase) of bovine kidney mitochondria. Biochem. Biophys. Res. Commun. 105, 320-327.
6. 3 1α-hydroxylase. Gerontology 42, 67-77.
7. 3 1α-hydroxylase from rat kidney mitochondria. FEBS Lett. 419, 45-48.
8. 3 synthesis. Science 277, 1827-1830.
9. 3 1α-hydroxylase gene in patients with pseudovitamin D-deficient rickets. N. Engl. J. Med. 338, 653-661.
10. 3-1α-hydroxylase cDNA. Proc. Natl Acad. Sci. USA 94, 12920-12925.
11. 3 I alpha-hydroxylase. Biochem. Biophys. Res. Commun. 239, 527-533.
12. 12. Fu, G.K., Martin, D.L., Zhang, Y.H., Bikle, D.D., Shackleton, C.H.L., Miller, W.L. & Portale, A.A. (1997) Cloning of human 25-hydroxyvitamin D-1α-hydroxylase and mutations causing vitamin D dependent rickets type I. Mol. Endocrinol. 11, 1961-1970.
13. 13. Akiyoshi-Shibata, M., Sakaki, T., Ohyama, Y., Noshiro, M., Okuda, K. & Yabusaki, Y. (1994) Further oxidation of hydroxycalcidiol by calcidiol 24-hydroxylase. Eur. J. Biochem. 224, 335-343.
14. 14. Wada, A., Mathew, P.A., Barnes, H., Sanders, D., Estabrook, R.W. & Waterman, M.R. (1991) Expression of functional bovine cholesterol side chain cleavage cytochrome P450 (P450scc) in Escherichia coli. Arch. Biochem. Biophys. 290, 376-380.
15. 15. Pikuleva, I.A., Brorkhem, I. & Waterman, M.R. (1997) Expression, purification, and enzymatic properties of recombinant human cytochrome P450c27 (CYP27). Arch. Biochem. Biophys. 343, 123-130.
16. 16. Blattner, F.R., Plunkett III, G., Bloch, C.A., Perna, N.T., Burland, V., Rilley, M., Collado-Vides, J., Glasner J.D., Rode, C.K., Mayhew, G.F., Gregor, J., Davis, N.W., Kirkpatrick, H.A., Goeden, M.A., Rose, D.J., Mau, B. & Shao, Y. (1997) The complete genome sequence of Escherichia coli K-12. Science 277, 1453-1462.
17. 17. Sakaki, T., Kominami, S., Hayashi, K., Akiyoshi-Shibata, M. & Yabusaki, Y. (1996) Molecular engineering study on electron transfer from NADPH-P450 reductase to rat mitochondrial P450c27 in yeast microsomes. J. Biol. Chem. 271, 26209-26213.
18. 18. Barnes, H.J., Arlotto, M.P. & Waterman, M.R. (1991) Expression and enzymatic activity of recombinant cytochrome P450, 17α-hydroxylase in Escherichia coli. Proc. Natl Acad. Sci. USA 88, 5597-5601.
19. 19. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265-275.
20. 3 24-hydroxylase. FEBS Lett. 278, 195-198.
21. 3-24-hydroxylase, a multicatalytic enzyme. Biochemistry 35, 8465-8472.
22. 3-1α-hydroxytase in porcine hepatic tissue: Subcellular localization to both mitochondria and microsomes. Proc. Natl Acad. Sci. USA 87, 6009-6013.
23. 23. Hays, S.J., Tobes, M.C., Gildersleeve, D.L., Wieland, D.M. & Beierwaltes, W.H. (1985) Structure - activity relationship study of the inhibition of adrenal cortical 11β-hydroxylase by new metyrapone analogues. J. Med. Chem. 27, 15-19.
24. 24. Ishida, M., Bulos, B., Takamoto, S. & Sacktor, B. (1987) Hydroxylation of 25-hydroxyvitamin D3 by renal mitochondria from rats of different ages. Endocrinology 121, 443-448.
25. 25. Pedersen, J.I., Hagenfeldt, Y. & Bjorkhem, I. (1988) Assay and properties of 25-hydroxyvitamin D3, 23-hydroxylase. Properties of guinea-pig kidney 25-hydroxyvitamin D3, 1α-hydroxylase assayed by isotope dilution - mass spectrometry. Biochem. J. 250, 521-526.
26. 26. Gray, R.W. & Ghazarian, J.G. (1989) Solubilization and reconstitution of kidney 25-hydroxyvitamin D3, 1α-and 24-hydroxylases from vitamin-D-replete pigs. Biochem. J. 259, 561-568.
27. 3 in the liver of fetal rats. Comp. Biochem. Physiol. 109, 1-7.
28. 28. Wecksler, W.R. & Norman, A.W. (1980) Biochemical properties of 1α,25-dihydroxyvitamin D receptors. J. Steroid Biochem. 13, 977-989.
29. 29. Kumar, R., Wiesner, R., Scott, M. & Go, V.L. (1982) Physiology of 24,25-dihydroxyvitamin D3 in normal human subjects. Am. J. Physiol. 243, 370-374.
30. 3 and its analogs. J. Bone Miner. Res. 9, 1277-1288.
31. 3 administration of parameters of calcium metabolism. J. Clin. Endocrinol. Metab. 69, 467-469.
32. 3. Proc. Natl Acad. Sci. USA 91, 10014-10018.