Seeing the light: Calcium imaging in cells for drug discovery

Drug Discovery Today

Volumn 5, Issue SUPPL.1, 2000, Pages 15-19

  Mattheakis, Larry C ^a   Ohler, Lynne D ^a  

^a AFFYMAX RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0002362484     PISSN: 13596446     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1359-6446(00)01493-8     Document Type: Review

References (28)

1. Carafoli, E. (1987) Intracellular calcium homeostasis. Annu. Rev. Biochem. 56, 395-433
2. Tsien, R.W. and Tsien, R.Y. (1990) Calcium channels, stores and oscillations. Ann. Rev. Cell Biol. 6, 715-760
3. Bootman, M.D. and Berridge, M.J. (1995) The elemental principles of calcium signaling. Cell 83, 675-678
4. Berridge, M.J. et al. (1998) Calcium - a life and death signal. Nature 395, 645-648
5. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3450
6. Gonzalez, J.E. et al. (1999) Cell-based assays and instrumentation for screening ion-channel targets. Drug Discovery Today 4, 431-439
7. Minta, A. et al. (1989) Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J. Biol. Chem. 264, 8171-8178
8. Kao, J.P. et al. (1989) Photochemically generated cytosolic calcium pulses and their detection by fluo-3. J. Biol. Chem. 264, 8179-8184
9. Lin, K. et al. (1999) Rapid measurements of intracellular calcium using a fluorescence plate reader. Bio Techniques 2, 318-322
10. Schroeder, K.S. and Neagle, B.D. (1996) FLIPR: a new instrument for accurate, high throughput optical screening. J. Biomol. Screening 1, 75-80
11. Schroeder, K. and Neagle, B. (1994) Method and apparatus for quantitative fluorescence measurements. US 5355215
12. Ridgway, E.B. and Ashley, C.C. (1967) Calcium transients in single muscle fibers. Biochem. Biophys. Res. Commun. 29, 229-234
13. 2+ concentration in the endoplasmic reticulum of intact cells. EMBO J. 14, 5467-5475
14. De Giorgi, F. et al. (1996) Targeting aequorin and green fluorescent protein to intracellular organelles. Gene 173, 113-117
15. 2+]c). A critical evaluation. J. Biol. Chem. 270, 9896-9903
16. Stables, J. et al. (1997) A bioluminescent assay for agonist activity at potentially any G-protein-coupled receptor. Anal. Biochem. 252, 115-126
17. Maeda, A. et al. (1996) Generation of cell transfectants expressing cardiac calcium ion channel and calcium indicator protein aequorin. Anal. Biochem. 242, 31-39
18. Kendall, J.M. and Badminton, M.N. (1998) Aequorea victoria bioluminescence moves into an exciting new era. Trends Biotechnol. 16, 216-224
19. Rao, A. et al. (1997) Transcription factors of the NFAT family: regulation and function. Annu. Rev. Immunol. 15, 707-747
20. 2+-regulated transcription factor phosphorylated by calmodulin dependent kinases. Science 252, 1427-1430
21. Bronstein, I. et al. (1994) Chemiluminescent and bioluminescent reporter gene assays. Anal. Biochem. 219, 169-181
22. Zlokarnik, G. et al. (1998) Quantitation of transcription and clonal selection of single living cells with β-lactamase as reporter. Science 279, 84-88
23. Mattheakis, L.C. et al. (1999) Expression of Cre recombinase as a reporter of signal transduction in mammalian cells. Chem. Biol. 6, 835-844
24. 2+ response amplitude and duration. Nature 386, 855-858
25. Dolmetsch, R.E. et al. (1998) Calcium oscillations increase the efficiency and specificity of gene expression. Nature 392, 933-936
26. 2+ spike frequency can optimize gene expression. Nature 392, 936-941
27. q-coupled receptors in lymphoid and non-lymphoid cells. J. Biol. Chem. 271, 10429-10432
28. Li, W et al. (1999) A calcium-sensitive magnetic resonance imaging contrast agent. J. Am. Chem. Soc. 121, 1413-1414