Simultaneous dual-color and dual-polarization imaging of single molecules

Applied Physics Letters

Volumn 77, Issue 24, 2000, Pages 4052-4054

  Cognet, Laurent ^a   Harms, Gregory S ^a   Blab, Gerhard A ^a   Lommerse, Piet H M ^a   Schmidt, Thomas ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001759698     PISSN: 00036951     EISSN: None     Source Type: Journal    
DOI: 10.1063/1.1332414     Document Type: Article

References (17)

1. S. Weiss, Science 283, 1676 (1999).
2. T. Ha, T. Enderle, D. F. Ogletree, D. S. Chemla, P. R. Selvin, and S. Weiss, Proc. Natl. Acad. Sci. U.S.A. 93, 6264 (1996).
3. G. J. Schütz, W. Trabesinger, and T. Schmidt, Biophys. J. 74, 2223 (1998).
4. Th. Förster, Ann. Phys. (Leipzig) 6, 55 (1948).
5. A. A. Deniz, M. Dahan, J. R. Grunwell, T. Ha, A. E. Faulhaber, D. S. Chemla, S. Weiss, and P. G. Schultz, Proc. Natl. Acad. Sci. U.S.A. 96, 3670 (1999).
6. T. Ha, A. Y. Ting, J. Liang, W. B. Caldwell, A. A. Deniz, D. S. Chemla, P. G. Schultz, and S. Weiss, Proc. Natl. Acad. Sci. U.S.A. 96, 893 (1999).
7. X. Zhuang, L. E. Bartley, H. P. Babcock, R. Russel, T. Ha, D. Herschlag, and S. Chu, Science 288, 2048 (2000).
8. T. Ha, X. Zhuang, H. D. Kim, J. W. Orr, J. R. Williamson, and S. Chu, Proc. Natl. Acad. Sci. U.S.A. 96, 9077 (1999).
9. S. Brasselet, E. J. G. Petermann, A. Miyawaki, and W. E. Moerner, J. Phys. Chem. B 104, 3676 (2000).
10. K. J. Kinosita, H. Itoh, S. Ishiwata, K. Hirano, T. Nishizaka, and T. Hayakawa, J. Cell Biol. 115, 67 (1991).
11. G. S. Harms, M. Sonnleitner, G. J. Schütz, H. J. Gruber, and T Schmidt, Biophys. J. 77, 2864 (1999).
12. T. Ha, T. Enderle, D. S. Chemla, P. R. Selvin, and S. Weiss, Phys. Rev. Lett. 77, 3979 (1996).
13. T. Ha, J. Glass, T. Enderle, D. S. Chemla, and S. Weiss, Phys. Rev. Lett. 80, 2093 (1998).
14. N. M. Green, Methods Enzymol. 184, 51 (1990).
15. T. Schmidt, G. J. Schütz, W. Baumgartner, H. J. Gruber, and H. Schindler, J. Phys. Chem. 99, 17 6621995).
16. The detection efficiency is defined as the ratio of the number of photon-counts detected by the CCD camera and the number of photons emitted by the fluorophore. It is calculated from the polarization-dependent transmission/reflexion spectra of the wedge and optical filters involved, from the emission spectra of the fluorophores, and from the wavelength-dependent quantum efficiency of the CCD camera (>0.9 counts/photon) and finally from the numerical aperture of the microscope objective.
17. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd edition (Kluwer/Plenum, New York, 1999), Chap. 13, p. 367-391.