Expression, partial purification and functional properties of the muscle-specific calpain isoform p94

European Journal of Biochemistry

Volumn 265, Issue 2, 1999, Pages 839-846

  Branca, Donata ^a   Gugliucci, Arianna ^a   Bano, Daniele ^a   Brini, Marisa ^a   Carafoli, Ernesto ^a  

^a Department of Biological Chemistry ^*

Author keywords

Baculovirus; Calcium; Calpain; p94

Indexed keywords

CALCIUM; CALPAIN; HISTIDINE;

ARTICLE; AUTOLYSIS; BACULOVIRUS; BINDING AFFINITY; CALCIUM CELL LEVEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION;

ANIMALS; BACULOVIRIDAE; CALCIUM; CALPAIN; CELLS, CULTURED; CLONING, MOLECULAR; ENZYME STABILITY; HUMANS; ISOENZYMES; MICROSCOPY, FLUORESCENCE; MICROSCOPY, PHASE-CONTRAST; MUSCLE PROTEINS; MUSCLE, SKELETAL; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; RATS; RECOMBINANT PROTEINS; SPODOPTERA;

INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 0001493462     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00817.x     Document Type: Article

References (17)

1. 1. Richard, I., Broux, O., Allamand, V., Fougerousse, F., Chiannikulchai, N., Bourg, N., Brenguier, L., Devaud, C., Pasturaud, P., Roudaut, C., Hillaire, D., Passos-Bueno, M.-R., Zatz, M., Tischfield, J.A., Fardeau, M., Jackson, C.E., Cohen, D. & Beckmann, J.S. (1995) Mutations in the proteolytic enzyme calpain 3 cause limb-girdle muscular dystrophy type 2A. Cell 81, 27-40.
2. 2. Baghdiguian, S., Martin, M., Richard, I., Pons, F., Astier, C., Bourg, N., Hay, R.T., Chemaly, R., Halaby, G., Loiselet, J., Anderson, L.V.B., Lopez de Munain, A., Fardeau, M., Mangeat, P., Beckmann, J.S. & Lefranc, G. (1999) Calpain 3 deficiency is associated with myonuclear apoptosis and profound perturbation of the [κBα/NF-κB pathway in limb-girdle muscular dystrophy type 2A. Nat. Med. 5, 503-511.
3. 3. Sorimachi, H., Ishiura, S. & Suzuki, K. (1997) Structure and physiological function of calpains. Biochem. J. 328, 721-732.
4. 4. Sorimachi, H., Imajoh-Ohmi, S., Emori, Y., Kawasaki, H., Ohno, S., Minami, Y. & Suzuki, K. (1989) Molecular cloning of a novel mammalian calcium-dependent protease distinct from both m- and μ-types. J. Biol. Chem. 264, 20106-20111.
5. 5. Kinbara, K., Ishiura, S., Tomioka, S., Sorimachi, H., Jeong, S.-Y., Amano, S., Kawasaki, H., Kolmerer, B., Kimura, S., Labeit, S. & Suzuki, K. (1998) Purification of native p94, a muscle-specific calpain, and characterization of its autolysis. Biochem. J. 335, 589-596.
6. 6. Sorimachi, H., Kinbara, K., Kimura, S., Takahashi, M., Ishiura, S., Sasagawa, N., Sorimachi, N., Tagawa, K., Maruyama, K. & Suzuki, K. (1995) Muscle-specific calpain, p94, responsible for limb girdle muscular dystrophy type 2A, associates with connectin through IS2, a p94-specific sequence. J. Biol. Chem. 270, 31158-31162.
7. 7. Sorimachi, H., Toyama-Sorimachi, N., Saido, T.C., Kawasaki, H., Sugita, H., Miyasaka, M., Arahata, K., Ishiura, S. & Suzuki, K. (1993) Muscle-specific calpain, p94, is degraded by autolysis immediately after translation, resulting in disappearance from muscle. J. Biol. Chem. 268, 10593-10605.
8. 8. Anderson, L.V.B., Davison, K., Moss, J.A., Richard, I., Fardeau, M., Tomé, F.M.S., Hübner, C., Lasa, A., Colomer, J. & Beckmann, J.S. (1998) Characterization of monoclonal antibodies to calpain 3 and protein expression in muscle from patients with limb-girdle muscular dystrophy type 2A. Am. J. Pathol. 153, 1169-1179.
9. 9. Cantini, M., Massimino, M.L., Catani, C., Rizzuto, R., Brini, M. & Carraro, U. (1994) Gene transfer into satellite cells from regenerating muscle: bupivacaine allows β-GAL transfection and expression in vitro and in vivo. In Vitro Cell. Dev. Biol. 30A, 131-133.
10. 10. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
11. 11. Merril, C.R., Goldman, D. & Van Keuren, M. (1984) Gel protein strains: silver stain. Methods Enzymol. 104, 441-447.
12. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 260, 3440-3450.
13. 13. Fabiato, A. (1988) Computer programs for calculating total from specified free or free from specified total ionic concentrations in aqueous solutions containing multiple metals and ligands. Methods Enzymol. 157, 378-417.
14. 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis. J. Biochem. 95, 511-519.
15. 15. Vilei, E.M., Calderara, S., Anagli, J., Berardi, S., Hitomi, K., Maki, M. & Carafoli, E. (1997) Functional properties of recombinant calpain I and of mutants lacking domains III and IV of the catalytic subunit. J. Biol. Chem. 272, 25802-25808.
16. 16. Meyer, S.L., Bozyczko-Coyne, D., Mallya, S.K., Spais, C.M., Bihovsky, R., Kawooya, J.K., Lang, D.M., Scott, R.W. & Siman, R. (1996) Biologically active monomeric and heterodimeric recombinant human calpain I produced using the baculovirus expression system. Biochem. J. 314, 511-519.
17. 17. Ono, Y., Shimada, H., Sorimachi, H., Richard, I., Saido, T.C., Beckmann, J.S., Ishiura, S. & Suzuki, K. (1998) Functional defects of a muscle-specific calpain, p94, caused by mutations associated with limb-girdle muscular dystrophy type 2A. J. Biol. Chem. 273, 17073-17078.