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note
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5, respectively) was added to a reaction solution containing Fe(TDCPPS) (0.04 mM) and CBZ (1 mM, introduced as a 0.1 M solution in methanol) in buffered aqueous solution (5 mL). Reactions at pH 2-3 were performed in formate buffer (0.1 M), at pH 4-5 in acetate buffer (0.1 M), and at pH 6-7 in phosphate (0.1 M), and the pH of the reaction solutions was adjusted by adding either HCl (3 N) or NaOH (3 N) solutions whenever it was necessary. The reaction mixture was stirred in air for 30 min at 25 °C, and then analyzed by Orom Vintage 2000 HPLC equipped with a variable wavelength UV-200 detector. CBZ and its epoxide derivative were separated on Waters Symmetry C18 column, 5 μM, eluted by a mixture of methanol/water, 60:40 (v/v), with retention times of 4.8 and 3.5 min, respectively. Detection was made at 215 nm. Calculation of the conversions of CBZ and the yields of CBZ-10,11- oxide was determined on the corresponding calibration curves of CBZ and the authentic CBZ-10,11-oxide prepared by a literature method. See ref 6 for the preparation of CBZ-10,11-oxide as an authentic sample.
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