Comparison of promoters suitable for regulated overexpression of β-galactosidase in the alkane-utilizing yeast Yarrowia hpolytica

Biotechnology and Bioprocess Engineering

Volumn 5, Issue 5, 2000, Pages 320-326

  Juretzek, Thomas ^a   Wang, Hui Jie ^b   Nicaud, Jean Marc ^b   Mauersberger, Stephan ^a   Barth, Gerold ^a  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^b CEMAGREF   (France)

Author keywords

Expression vectors; lacZ expression; Regulated promoters; Yarrowia lipolytica

Indexed keywords

EID: 0001083310     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02942206     Document Type: Article

References (34)

1. Barth, G. and C. Gaillardin (1996) Yarrowia lipolytica. pp. 313-388. In: K. Wolf (ed.) Nonconventional yeasts in biotechnology. A Handbook. Springer-Verlag, Berlin, Germany.
2. Barth, G. and C. Gaillardin (1997) Physiology and genetics of the dimorphic fungus Yarrowia lipolytka. FEMS Micobiol. Rev. 19: 219-237.
3. Gaillardin, C. and H. Heslot (1988) Genetic engineering in Yarrowia lipolytica. J. Basic Microbiol. 28: 161-174.
4. Romanos, M., C. Scorer, and J. Clare (1992) Foreign gene expression in yeast. Yeast 8: 423-488.
5. Sudbery, P. (1994) Hon-Saccharomyces yeasts. Yeast 10: 1707-1726.
6. Mtiller, S., T. Sandal, P. Kamp-Hansen, and H. Dalboge (1998) Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyuveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Yeast 7: 1267-1283.
7. Fournier, P., L. Guyaneux, M. Chasles, and C. Gaillardin (1991) Scarcity of ars sequences isolated in a morphogenesis mutant of the yeast Yarrowia lipolytka. Yeast 7: 25-36.
8. Fournier, P., A. Abbas, M. Chasles, B. Kudla, D. M. Ogrydziak, D. Yaver, J.-W. Xuan, A. Peito, A.-M. Ribet, C. Feynerol, E He, and C. Gaillardin (1993) Colocalization of centromeric and replicative functions on autonomously replicating sequences isolated from the yeast Yarrowia lipolytica. Proc. Natl. Acad. Sci. USA 90: 4912-4916.
9. Matsuoka, M., M. Matsubara, H. Daidoh, T. Imanaka, K. Uchida, and S. Aiba (1993) Analysis of regions essential for the function of chromosomal replicator sequences from Yarrowia lipolytica. Mol. Gen. Genet. 237: 327-333.
10. Vernis, L., A. Abbas, M. Chasles, C. M. Gaillardin, C. Brun, J. A. Huberman, and P. Fournier (1997) An origin of replication and a centromere are both needed to establish a replicative plasmid in the yeast Yarrowia lipolytica. Mol. Cell. Biol. 17: 1995-2004.
11. Vernis, L., M. Chasles, P. Pasero, A. Lepingle, C. Gaillardin, and P. Fournier (1999) Short DNA fragments without sequence similarity are initiation sites for replication in the chromosome of the yeast Yarrowia lipolytica. Mol. Cell. Biol. 10: 757-769.
12. Le Dall, M.-T, J.-M. Nicaud, and C. Gaillardin (1994) Multi-copy integration in the yeast Yarrowia lipolytica. Curr. Genet. 26: 38-44.
13. Juretzek, T., M. T. Le Dall, S. Mauersberger, C. Gaillardin, G. Barth, and J. M. Nicaud (2000) Vectors for gene expression and amplification in the yeast Yarrowia lipolytica. Yeast in press.
14. Matoba, S., J. Fukuyama, R. A. Wing, and D. M. Ogrydziak (1988) Intracellular precursors and secretion of alkaline extracellular protease of Yarrowia lipolytica. Mol. Cell. Biol. 8: 4904-4916.
15. Fabre, E., J. M. Nicaud, M. C. Lopez, and C. Gaillardin (1991) Role of the proregion in the production and secretion of the Yarrowia lipolytica alkaline extracellular protease. J. Biol. Chem. 266: 3782-3790.
16. Matoba, S., K. A. Morano, D. J. Klionsky, K. Kim, and D. M. Ogrydziak (1997) Dipeptidyl aminopeptidase processing and biosynthesis of extracellular protease from Yarrowia lipolytica.Microbiology 143: 3263-3272.
17. Blanchin-Roland, S., R. Cordero Otero, and C. Gaillardin (1994) Two upstream UAS control expression of the XPR2 gene encoding an extracellular alkaline protease in the yeast Yarrowia lipolytica. Mol. Cell. Biol. 14: 327-338.
18. Park, C. S., C. C. Chang, J. Y. Kim, D. M. Ogrydziak, and D. D. Ryu (1997) Expression, secretion, and processing of rice a-amylase in the yeast Yarrowia lipolytica. J. Biol. Chem. 272:6876-6881.
19. Chang, C. C., D. D. Ryu, C. S. Park, and J. Y. Kim (1998) Improvement of heterologous protein productivity using recombinant Yarrowia lipolytica and cyclic fed-batch process strategy. Biotechnol. Bioeng. 59: 379-385.
20. Madzak, C., S. Blanchin-Roland, R. R. Cordero-Otero, and C. Gaillardin (1999) Functional analysis of upstream regulating regions from the Yarrowia lipolytica XPRZ promoter. Microbiology 145: 75-87.
21. Barth, G. and T. Scheuber (1993) Cloning of the isocitrate lyase gene (ICLI) from Yarrowia lipolytica and characterization of the deduced protein. Mol. Gen. Genet. 241: 422-430.
22. Berninger, G., R. Schmidtchen, G. Casel, A. Knoerr, K. Rautenstrauss, W.-H. Kunau, and E. Schweizer (1993) Structure and metabolic control of the Yarrowia lipoly-tica peroxisomal 3-oxoacyl-CoA-thiolase gene. Eur. J. Biochem. 216: 607-613.
23. Wang, H., A. Le Clainche, M. T. Le Dall, Y. Wache, Y. Pagot, J. M. Belin, C. Gaillardin, and J. M. Nicaud (1998) Cloning and characterization of the peroxisomal acyl CoA oxidase ACO3 gene from the alkane-utilizing yeast Yarrowia lipolytica. Yeast 14: 1373-1386.
24. Wang, H. J., M. T. Le Dall, Y. Wach, C. Laroche, J. M. Belin, C. Gaillardin, and J. M. Nicaud (1999) Evaluation of acyl coenzyme A oxidase (Aox) isozyme function in the n-alkane-assimilating yeast Yarrowia lipolytica. J. Bacteriol. 181:5140-5148.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis (1989) Molecular cloning. A laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
26. Mauersberger, S., M. Ohkuma, W.-H. Schunck, and M. Takagi (1996) Candida maltosa. pp. 411-580. In: K. Wolf (ed.) Nonconventional yeasts in biotechnology. A Handbook. Springer-Verlag, Berlin, Germany.
27. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (1998) Current Protocols in Molecular Biology, Vol. 1, Harvard Medical School, Massachusetts, USA.
28. Juretzek, T. (1999) Entwicklung von Wirts-Vektor-Systemen zur heterologen Expression von Proteinen in der nichtkonventionellen Hefe Yarrowia lipolytica und ihre Anwendung für die Cytochrom P450-katalysierte Stoffumwandlung, PhD thesis, Technische Universität Dresden, Germany.
29. Schmidtchen, R. (1994) Untersuchungen zur Regulation des 3-Oxoacyl-CoA-Thio-lase-I-Gens aus Yarrowia lipoly-tica, PhD thesis, Friedrich-Alexander-Universität, Erlangen, Germany.
30. Madzak, C., B. Tréton, and S. Blanchin-Roland (2000) Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. J. Mol. Microbiol. Biotechnol. 2: 207-216.
31. Sanger, F., S. Nicklen, and A. R. Coulson (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74: 5463-5467.
32. Gaillardin, C. and A.-M. Ribet (1987) LEUZ directed expression of fi-galactosidase activity and phleomycin resis-tance in Yarrowia lipolytica. Curr. Genet. 11: 369-375.
33. Pagot, Y, A. Endrizzi, J. M. Nicaud, and J. M. Belin (1997) Utilization of an auxotrophic strain of the yeast Yarrowia lipolytica to improve gamma-decalactone production yields. Lett. Appl. Microbiol. 25: 113-116.
34. Gerber, J. (1999) Untersuchungen zur Optimierung des Elektronentransportsystems für die Cytochrom P450 katalysierte Biotransformation von Steroiden in Yarrowia lipolytica. Diploma thesis, Technische Universität Dresden, Germany.