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A 70-nm-diameter vesicle packed with 0.2 M neurotransmitter contains ∼30 000 molecules, ∼2-fold higher than the detection limit for CPP-glycine. The components of such a vesicle, originally contained in a volume of ∼0.2 aL, would diffuse longitudinally and radially in a 620-nm-i.d. capillary to a volume of ∼80 fL in 200 s. The resulting concentration at the detection site thus would be ∼600 nM.
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Fluorescence detection has been used to measure the contents of individual secretory granules from other cell types, in which the amount of material is 10-100-fold greater than that contained in neuronal vesicles.
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