Probing single secretory vesicles with capillary electrophoresis

Science

Volumn 279, Issue 5354, 1998, Pages 1190-1193

  Chiu, Daniel T ^a   Lillard, Sheri J ^a   Scheller, Richard H ^a   Zare, Richard N ^a   Rodriguez Cruz, Sandra E ^b   Williams, Evan R ^b   Orwar, Owe ^c   Sandberg, Mats ^c   Lundqvist, J Anders ^c  

^a STANFORD UNIVERSITY   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

NAPHTHALENE DERIVATIVE; PEPTIDE;

APLYSIA; ARTICLE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; FLUORESCENCE; LASER; LYSIS; MEMBRANE VESICLE; NONHUMAN; OPTICS; PRIORITY JOURNAL; SECRETORY GRANULE;

AMINES; AMINO ACIDS; ANIMALS; APLYSIA; CYTOPLASMIC GRANULES; ELECTROPHORESIS, CAPILLARY; MASS SPECTROMETRY; NAPHTHALENES; PEPTIDES; POTASSIUM CYANIDE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TAURINE;

APLYSIA; GASTROPODA;

EID: 0032549122     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.279.5354.1190     Document Type: Article

References (28)

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23. Exact masses were determined from four replicate measurements with a mass accuracy of better than 10 parts per million using internal calibration standards. Elemental compositions were assigned on the basis of the masses of the common elements and were constrained by the measured isotope distributions.
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28. We thank P. Schnier, R. Jockusch, and J. Klassen for technical assistance with mass spectrometry. We also thank G. T. Nagle and S. D. Painter for generously providing us with purified atrial gland peptides. S.J.L. acknowledges support from NIH for a postdoctoral fellowship (GM18386). O.O. is supported by the Swedish Natural Science Research Council (NFR) (10481-305, -308, and -309) and by the Swedish Foundation for Strategic Research (SSF). M.S. is supported by NFR (01-905-313). This work is supported by the International Joint Research Program (NEDO) of Japan, the U.S. National Institute on Drug Abuse (DA09873), NSF (CHE-9258178), and NIH (1R29GM50336-01A2).