Simultaneous On-Line Characterization of Small Organic Molecules Derived from Combinatorial Libraries for Identity, Quantity, and Purity by Reversed-Phase HPLC with Chemiluminescent Nitrogen, UV, and Mass Spectrometric Detection

Analytical Chemistry

Volumn 70, Issue 16, 1998, Pages 3339-3347

  Taylor, Eric W ^a   Qian, Mark G ^a   Dollinger, Gavin D ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000595570     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac980402d     Document Type: Article

References (17)

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17. With ∼800 nitrogen atoms per molecule of BSA, 100 pmol of nitrogen is equivalent to ∼125 fmol (10 ng) of protein. This sensitivity with protein permits the quantitation of a protein at the femtomole or nanogram level without the need of an extinction coefficient.