Chemiluminescent nitrogen detection as a new technique for purity assessment of synthetic peptides separated by reversed-phase HPLC

Peptide Research

Volumn 9, Issue 1, 1996, Pages 40-44

  Bizanek, Roland ^a,b   Damon Manes J ^a   Fujinari, Eugène M ^a  

^a Advanced ChemTech   (United States)

^b Antek Instruments Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANGIOTENSIN II; C PEPTIDE; ENKEPHALIN; NITROGEN; PEPTIDE;

ARTICLE; CHEMISTRY; CHEMOLUMINESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; ISOLATION AND PURIFICATION; SYNTHESIS;

ANGIOTENSIN II; C-PEPTIDE; CHEMILUMINESCENT MEASUREMENTS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ENKEPHALINS; NITROGEN; PEPTIDES;

EID: 0029705338     PISSN: 10405704     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

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2. Frank, B.H., J.M. Pettee, R.E. Zimmerman and P.J. Burek. 1981. The production of human proinsulin and its transformation to human insulin and C-Peptide, p. 729-738. In D.H. Rich and E. Gross (Eds.), Peptides, Synthesis, Structure, Function. Proceedings of the Seventh American Peptide Symposium, Pierce Chemical Co., Rockford, IL.
3. Fujinari, E.M. and J.D. Manes. 1994. Nitrogen-specific detection of peptides in liquid chromatography with a chemiluminescent nitrogen detector. J. Chromatogr. A676:113-120.
4. Hearn, M.T.W. (Ed.). 1991. HPLC of peptides, proteins, and polynucleotides: contemporary topics and applications, VCH Publishers, New York.
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7. Meirifield, R.B. 1963. Solid phase synthesis 1. The synthesis of a tetrapeptide. J. Am. Chem. Soc. 5:2149-2154.
8. Roe, O.K. 1983. Comparison of amperometric and coulometric electrochemical detectors for HPLC through a figure of merit. Anal. Lett. 76:613-631.
9. Sedlack, B.J. 1993. Market for therapeutic peptides expected to surpass $350 million in 1994. Genetic Engineering News 13:6-7.
10. O.Steiner, V., K.O. Bornsen, M. Schar, E. Gassmann, S. Hoffstetter-Kuhn, H. Rink and M. Mutter. 1992. Analysis of synthetic peptides using MALD1 mass spectrometry. Pept. Res. 5:25-29.
11. Wang, S.S. 1973. p-AIkoxybenzyl alcohol resin and p-alkoxybenzyloxycarbonylhydrazide resin for solid phase synthesis of protected peptide fragments. J. Am. Chem. Soc. 95:1328-1333.
12. Wehr, C.T. 1991. Postcolumn reaction systems for fluorescence detection of polypeptides, p. 579-586. In High-Performance Liquid Chromatography of Peptides and Proteins: Separation, Analysis, and Conformation. CRC Press, Roca Raton, FL.