Liquid-liquid reversed micellar extraction for isolating enzymes: Studies on the purification of α-amylase

Process Biochemistry

Volumn 31, Issue 4, 1996, Pages 371-375

  Chang, Qing Long ^a   Chen, Jia Yong ^a  

^a INSTITUTE OF GEOLOGY AND GEOPHYSICS   (China)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0030019169     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/0032-9592(95)00072-0     Document Type: Article

References (10)

1. Luisi, P. L. & Laane, C., Solubilization of enzymes in apolar solvents via reversed micelles. Trends in Biotechnol., 4 (1986) 153-61.
2. Dekker, M., Van't Riet, K., Weijers, S. R., Baltussen, J. W. A., Laane, C. & Bijsterbosch, B. H., Enzyme recovery by liquid-liquid extraction using reversed micelles. Chem. Eng. J., 33 (1986) B27-33.
3. Goklen, K. E. & Hatton, T. A., Liquid-liquid extraction of low molecular weight protein by selective solubilization in reversed micelles. Sep. Sci. Technol., 22 (1987) 831-41.
4. Rahaman, R. S., Chee, J. Y., Cabrai, J. M. S. & Hatton, T. A., Recovery of an extracellular alkaline protease from whole fermentation broth using reversed micelles. Biotechnol. Progr., 4 (1988) 218-23.
5. Aires-Barros, M. R. & Cabral, J. M. S., Selective separation and purification of two lipases from Chromobacterium viscoum with AOT reversed micelles. Biotech. Bioeng., 38 (1991) 1302-7.
6. Goto, M. & Nakashio, F., New reversed micelles for extraction and separation of proteins. In Solvent Extraction in the Process Industries, Vol. 2, ed. D. H. Logsdail & M. J. Slater, Elsevier, London, 1993, pp. 946-53.
7. Jolivalt, C., Minier, M. & Renon, H., Extraction of α-chymotrypsin using reversed micelles. J. Colloid Interf. Sci., 135 (1990) 85-96.
8. Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J., Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193 (1951) 265-75.
9. Goklen, K. E. & Hatton, T. A., Protein extraction using reversed micelles. Biotechnol Progr., 1 (1985) 69-74.
10. Dekker, M., Hilhorst, R. & Laane, C., Isolating enzyme by reversed micelles. Anal. Biochem., 178 (1989) 217-26.