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0346749045
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note
-
A 5 mg sample of bBSA dissolved in 1 mL of PBS buffer was reacted with 2 mg of 2-iminothiolane·HCl (Sigma) and stored at 4 °C for 2 h with occasional vortex. The resulting solution was filtered through a size-exclusion centrifugal filter device (Micron YM-30, Millipore Corporation, Bedford, MA) and diluted to 1 mL in PBS buffer. 5,5′-Dithiobis(2-nitrobenzoic acid) (99%, Aldrich) was used to quantify the labeling degree of free thiol groups on the bBSA. A 4 mg amount of this reagent was reacted with 200 μL of the bBSA solution, and the labeling degree was determined from absorbance measurements at 278 and412 nm.
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21
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0033536470
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22
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0346749043
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-
-1, respectively, were used to determine the concentrations by UV/vis spectrophotometry
-
-1, respectively, were used to determine the concentrations by UV/vis spectrophotometry.
-
-
-
-
23
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0346749046
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-
note
-
p is the radius of the protein (∼5 nm).
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-
-
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25
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0346749044
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note
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-1.
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26
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0348009879
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Landes, C.; Burda, C.; Braun, M.; El-Sayed, M. A. J. Phys. Chem. B 2001, 105, 4973-4978.
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Landes, C.1
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El-Sayed, M.A.4
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