Multiple gene products from a single vector: 'Self-cleaving' 2A peptides

Gene Therapy

Volumn 11, Issue 23, 2004, Pages 1673-1674

  Radcliffe, P A ^a   Mitrophanous, K A ^a  

^a OXFORD BIOMEDICA UK LTD   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; CD3 ANTIGEN; CYAN FLUORESCENT PROTEIN; GENE PRODUCT; IMMUNOGLOBULIN; PEPTIDE; POLYPROTEIN; PROTEIN SUBUNIT; T LYMPHOCYTE RECEPTOR; VIRUS VECTOR; YELLOW FLUORESCENT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENE THERAPY; GENE VECTOR; GENETIC TRANSCRIPTION; HUMAN; HUMAN CELL; IN VITRO STUDY; INTERNAL RIBOSOME ENTRY SITE; NONHUMAN; NOTE; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RNA TRANSLATION; STEM CELL; STOICHIOMETRY; T LYMPHOCYTE;

ANIMALIA;

EID: 9944227211     PISSN: 09697128     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.gt.3302361     Document Type: Note

References (7)

1. Szymczak AL et al. Correction of multigene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 2004; 22: 589-594; Epub 2004, April 4.
2. Donnelly ML et al. The 'cleavage' activities of foot-and-mouth disease virus 2A site-directed mutants and naturally occurring '2A-like' sequences. J Gen Virol 2001; 82: 1027-1041.
3. Julias JG, Hash D, Pathak VK. E-vectors: development of novel self-inactivating and self-activating retroviral vectors for safer gene therapy. J Virol 1995; 69: 6839-6846.
4. Szymczak AL et al. Addendum: correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 2004; 22: 760.
5. Wang B et al. T lymphocyte development in the absence of CD3 epsilon or CD3 gamma delta epsilon zeta. J Immunol 1999; 162: 88-94.
6. Shen Y et al. Triple transduction with adeno-associated virus vectors expressing tyrosine hydroxylase, aromatic-L-amino-acid decarboxylase, and GTP cyclohydrolase I for gene therapy of Parkinson's disease. Hum Gene Ther 2000; 11: 1509-1519.
7. Azzouz M et al. Multicistronic lentiviral vector-mediated striatal gene transfer of aromatic L-amino acid decarboxylase, tyrosine hydroxylase, and GTP cyclohydrolase I induces sustained transgene expression, dopamine production, and functional improvement in a rat model of Parkinson's disease. J Neurosci 2002; 22: 10302-10312.