Affinity labeling of a catalytic site, cysteine 247, in rat mercaptopyruvate sulfurtransferase by chloropyruvate as an analog of a substrate

Biochimie

Volumn 86, Issue 9-10, 2004, Pages 723-729

  Nagahara, Noriyuki ^a   Sawada, Nori ^a   Nakagawa, Toshio ^b  

^a NIPPON MEDICAL SCHOOL   (Japan)

^b YOKOHAMA CITY UNIVERSITY   (Japan)

Author keywords

Affinity labeling agent; Reaction mechanism

Indexed keywords

3 MERCAPTOPYRUVATE SULFURTRANSFERASE; 3 MERCAPTOPYRUVIC ACID; 5,5' DITHIOBIS(2 NITROBENZOIC ACID); ARGININE; CHLOROPYRUVIC ACID; CYSTEINE; GLYCINE; MERCAPTOETHANOL; MORPHINE SULFATE; PYRUVIC ACID DERIVATIVE; THIOSULFATE; UNCLASSIFIED DRUG;

AFFINITY LABELING; ARTICLE; ASSAY; BINDING SITE; CATALYSIS; ELECTROSPRAY MASS SPECTROMETRY; ENZYME INACTIVATION; KINETICS; NONHUMAN; RAT; SITE DIRECTED MUTAGENESIS; STOICHIOMETRY; TITRIMETRY;

AFFINITY LABELS; ANIMALS; CATALYTIC DOMAIN; CYSTEINE; PYRUVIC ACID; RATS; SULFURTRANSFERASES;

EID: 8844234229     PISSN: 03009084     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biochi.2004.08.002     Document Type: Article

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