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The product from the hybridization of complex 1b with 2 equivalents of complementary DNA exhibits a characteristic CD spectrum of a B-DNA: a negative band at λ = 253 nm, a crossover point at λ = 265 nm, and a positive band at λ = 277 nm (Figure 2b, solid line). This is very similar to the CD spectrum of the control duplex, b-c, which exhibits a negative band at λ = 255 nm, a crossover point at λ = 267 nm, and a positive band at λ = 280 nm (Figure 2b, dashed line); N. Berova, K. Nakanishi, R. W. Woody, Circular Dichroism: Principles and Applications, 2nd Ed., Wiley-VCH, Weinheim, 2000.
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8844254894
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The products of the hybridization of the mono(DNA)-functionalized Ru complexes 4 with their single-stranded DNA complements are observed to have similar electrophoretic mobility to that of a control duplex. Thus, the positive charge of the Ru center does not appear to affect the migration of the Ru-DNA samples.
-
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38
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8844228386
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-
note
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Please see the Supporting Information for changes in the fluorescence spectra of the Ru-bis(homopolymeric) sequence, 1a, upon hybridization to complementary DNA. The results show a 10% decrease in fluorescence intensity upon hybridization.
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See Supporting Information for results of molecular modeling studies of 8. The local energy minimum for 8 was obtained using the AMBER force field method.
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44
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3242656139
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Note added in Proof: After acceptance of this manuscript, a communication about the synthesis of DNA triangles with vertices of iron(II) bis(terpyridine) complexes has appeared: J. S. Choi, C. W. Kang, J. W. Yang, Y.-G. Kim, H. Han, J. Am. Chem. Soc. 2004, 126, 8606-8607.
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