Spontaneous formation of a molecular net assembly by using nucleotide complementarity

Chemistry Letters

Volumn , Issue 10, 1996, Pages 891-892

  Takenaka, Shigeori ^a,b   Funatu, Yousuke ^b   Kondo, Hiroki ^b  

^a KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0000064444     PISSN: 03667022     EISSN: None     Source Type: Journal    
DOI: 10.1246/cl.1996.891     Document Type: Article

References (9)

1. G. H. Keller and M. M. Manak, in DNA probes, Stockton Press, New York (1989).
2. J. Chen and N. C. Seeman, Nature, 350, 631 (1991).
3. 9 was dissolved in DMF with triethylamine and allowed to react with N-hydroxysuccinimide to yield 2 in 95% yield. Ester 2 was purified by silica gel chromatography using chloroform as an eluent.
4. A 100-fold molar excess of 2 was allowed to react with amino-linked oligonucleotide 1A or 1T in sodium bicarbonate buffer and DMSO. Mono-substituted products 3A and 3T and di-substituted products were obtained in a 2:1 ratio with 60% yield. Compounds 3A and 3T were purified by HPLC (Tosoh TSK-gel ODS-80Ts, 4.6 mm ID x 15 cm) to homogeneity: the retention times for 3A and 3T were 18.0 and 22.0 min, respectively, in a linear gradient of 10-30% of acetonitrile in 0.1 M triethylammonium acetate buffer (pH 7.0).
5. A titration curve of 3T in water saturated at 1/3 molar equivalent of ferrous ion.
6. M. Lieberman and T. Sasaki, J. Am. Chem. Soc., 113, 1470 (1991).
7. C. R. Cantor, M. M. Warshaw, and H. Shapiro, Biopolymers, 9, 1059 (1970).
8. We failed to estimate the mean molecular weight of the assembly by agarose gel electrophoresis because the metal complex of the assembly broke in this condition.
9. H. F. Case, J. Am. Chem. Soc., 68, 2574 (1946).